Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer
Gastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, developme...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.756085/full |
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| author | Haiyan Chen Qi Sun Cangang Zhang Junjun She Shuai Cao Meng Cao Nana Zhang Ayarick Vivian Adiila Jinjin Zhong Chengyun Yao Yili Wang Hongping Xia Hongping Xia Linhua Lan |
| author_facet | Haiyan Chen Qi Sun Cangang Zhang Junjun She Shuai Cao Meng Cao Nana Zhang Ayarick Vivian Adiila Jinjin Zhong Chengyun Yao Yili Wang Hongping Xia Hongping Xia Linhua Lan |
| author_sort | Haiyan Chen |
| collection | DOAJ |
| description | Gastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, development, and metastasis. Twenty-two kinds of immune cells were calculated by CIBERSORT from Gene Expression Omnibus (GEO) database. Subsequently, higher infiltration of macrophages M0 was discovered in GC compared with normal tissues. WGCNA was utilized to construct the network and then identify key modules and genes related to macrophages in TCGA. Finally, 18 hub genes were verified. In the PPI bar chart, the top 3 genes were chosen as hub genes involved in most pathways. On the TIMER and THPA websites, it is verified that the expression levels of CYBB, CD86, and C3AR1 genes in tumor tissues were higher than those in normal tissues. These genes may work as biomarkers or targets for accurate diagnosis and treatment of GC in the future. Our findings may be a new strategy for the treatment of GC. |
| first_indexed | 2024-12-14T04:27:19Z |
| format | Article |
| id | doaj.art-ed260e0717bb43de8a09786d78ec767c |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-14T04:27:19Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-ed260e0717bb43de8a09786d78ec767c2022-12-21T23:17:11ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-12-01810.3389/fmolb.2021.756085756085Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric CancerHaiyan Chen0Qi Sun1Cangang Zhang2Junjun She3Shuai Cao4Meng Cao5Nana Zhang6Ayarick Vivian Adiila7Jinjin Zhong8Chengyun Yao9Yili Wang10Hongping Xia11Hongping Xia12Linhua Lan13Institute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Pathology, School of Basic Medical Sciences and Sir Run Run Hospital and Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing, ChinaDepartment of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi’an Jiaotong University, Xi’an, ChinaDepartment of High Talent and General Surgery and Center for Gut Microbiome Research and Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong University, Xi’an, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaInstitute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaInstitute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaInstitute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaInstitute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaJiangsu Cancer Hospital and the Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Institute of Cancer Research, Nanjing, ChinaInstitute for Cancer Research, School of Basic Medical Science of Xi’an Jiaotong University, Xi’an, ChinaDepartment of Pathology, School of Basic Medical Sciences and Sir Run Run Hospital and Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing, ChinaDepartment of High Talent and General Surgery and Center for Gut Microbiome Research and Med-X Institute, the First Affiliated Hospital of Xi’an Jiao Tong University, Xi’an, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaGastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, development, and metastasis. Twenty-two kinds of immune cells were calculated by CIBERSORT from Gene Expression Omnibus (GEO) database. Subsequently, higher infiltration of macrophages M0 was discovered in GC compared with normal tissues. WGCNA was utilized to construct the network and then identify key modules and genes related to macrophages in TCGA. Finally, 18 hub genes were verified. In the PPI bar chart, the top 3 genes were chosen as hub genes involved in most pathways. On the TIMER and THPA websites, it is verified that the expression levels of CYBB, CD86, and C3AR1 genes in tumor tissues were higher than those in normal tissues. These genes may work as biomarkers or targets for accurate diagnosis and treatment of GC in the future. Our findings may be a new strategy for the treatment of GC.https://www.frontiersin.org/articles/10.3389/fmolb.2021.756085/fullmacrophagegastric cancerWGCNAimmune infiltrationhub genes |
| spellingShingle | Haiyan Chen Qi Sun Cangang Zhang Junjun She Shuai Cao Meng Cao Nana Zhang Ayarick Vivian Adiila Jinjin Zhong Chengyun Yao Yili Wang Hongping Xia Hongping Xia Linhua Lan Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer Frontiers in Molecular Biosciences macrophage gastric cancer WGCNA immune infiltration hub genes |
| title | Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer |
| title_full | Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer |
| title_fullStr | Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer |
| title_full_unstemmed | Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer |
| title_short | Identification and Validation of CYBB, CD86, and C3AR1 as the Key Genes Related to Macrophage Infiltration of Gastric Cancer |
| title_sort | identification and validation of cybb cd86 and c3ar1 as the key genes related to macrophage infiltration of gastric cancer |
| topic | macrophage gastric cancer WGCNA immune infiltration hub genes |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.756085/full |
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