Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria
Introduction. Research regarding the role of the IL-12 cytokine family in modulating immune and inflammatory responses is continuously evolving. In this study, the contribution of the p35 and p40 subunits as monomers (noted as IL-12p35 and IL-12p40) and heterodimers (noted as IL-12p70 or IL-12p35/p4...
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MDPI AG
2024-03-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/14/3/295 |
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| author | Corina Daniela Ene Milena Tocut Mircea Tampa Simona Roxana Georgescu Clara Matei Iulia Maria Teodora Leulescu Ilinca Nicolae Cosmin Ene |
| author_facet | Corina Daniela Ene Milena Tocut Mircea Tampa Simona Roxana Georgescu Clara Matei Iulia Maria Teodora Leulescu Ilinca Nicolae Cosmin Ene |
| author_sort | Corina Daniela Ene |
| collection | DOAJ |
| description | Introduction. Research regarding the role of the IL-12 cytokine family in modulating immune and inflammatory responses is continuously evolving. In this study, the contribution of the p35 and p40 subunits as monomers (noted as IL-12p35 and IL-12p40) and heterodimers (noted as IL-12p70 or IL-12p35/p40) was analysed in the pathophysiology and progression of chronic spontaneous urticaria (CSU). Materials and methods. We conducted a longitudinal, case–control study involving 42 CSU cases and 40 control cases comprising adults without associated conditions. Serial measurements were performed to assess the serum levels of IL-12p70, IL-12p35, and IL-12p40 at the onset of the disease (pre-therapy phase) and 6 weeks after the initiation of the treatment (post-therapy phase). Results. During the pre-therapeutic phase of CSU, elevated serum levels of IL-12 cytokine subtypes were detected compared to the control group. The relationship between IL-12 profiles and the course of CSU highlighted the pro-inflammatory role of IL-12p70 and the anti-inflammatory role of IL-12p35. Significant correlations were observed between IL-12p70 levels and the duration of the disease, as well as between IL-12 and the effectiveness of H1-antihistamines. Conclusions. The molecular background for the pleiotropic activities mediated by IL-12-derived cytokines in patients with CSU lies in the strict regulation of the production, signalling pathways, and cytokine-specific influences on the same pathophysiological events. The results of the present study suggest that the superficial layers of the skin serve as a cellular source of IL-12, a cytokine produced through antigenic stimulation. In patients with CSU, we identified independent, additive, or divergent functions of IL-12p70, IL-12p35, and IL-12p40, all relevant to systemic inflammation. These findings prove that the prototype programming of IL-12 is abnormal in CSU. |
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| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-04-24T18:06:29Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-ed28a4985fea4683be01d4a4293f51792024-03-27T13:50:12ZengMDPI AGJournal of Personalized Medicine2075-44262024-03-0114329510.3390/jpm14030295Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous UrticariaCorina Daniela Ene0Milena Tocut1Mircea Tampa2Simona Roxana Georgescu3Clara Matei4Iulia Maria Teodora Leulescu5Ilinca Nicolae6Cosmin Ene7Department of Nephrology, ‘Carol Davila’ Nephrology Hospital, 010731 Bucharest, RomaniaWolfson Medical Center, Holon 58100, IsraelDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, ‘Victor Babes’ Clinical Hospital for Infectious Diseases, 030303 Bucharest, RomaniaDepartment of Dermatology, ‘Victor Babes’ Clinical Hospital for Infectious Diseases, 030303 Bucharest, RomaniaDepartment of Urology, ‘Carol Davila’ University of Medicine and Pharmacy, 050474 Bucharest, RomaniaIntroduction. Research regarding the role of the IL-12 cytokine family in modulating immune and inflammatory responses is continuously evolving. In this study, the contribution of the p35 and p40 subunits as monomers (noted as IL-12p35 and IL-12p40) and heterodimers (noted as IL-12p70 or IL-12p35/p40) was analysed in the pathophysiology and progression of chronic spontaneous urticaria (CSU). Materials and methods. We conducted a longitudinal, case–control study involving 42 CSU cases and 40 control cases comprising adults without associated conditions. Serial measurements were performed to assess the serum levels of IL-12p70, IL-12p35, and IL-12p40 at the onset of the disease (pre-therapy phase) and 6 weeks after the initiation of the treatment (post-therapy phase). Results. During the pre-therapeutic phase of CSU, elevated serum levels of IL-12 cytokine subtypes were detected compared to the control group. The relationship between IL-12 profiles and the course of CSU highlighted the pro-inflammatory role of IL-12p70 and the anti-inflammatory role of IL-12p35. Significant correlations were observed between IL-12p70 levels and the duration of the disease, as well as between IL-12 and the effectiveness of H1-antihistamines. Conclusions. The molecular background for the pleiotropic activities mediated by IL-12-derived cytokines in patients with CSU lies in the strict regulation of the production, signalling pathways, and cytokine-specific influences on the same pathophysiological events. The results of the present study suggest that the superficial layers of the skin serve as a cellular source of IL-12, a cytokine produced through antigenic stimulation. In patients with CSU, we identified independent, additive, or divergent functions of IL-12p70, IL-12p35, and IL-12p40, all relevant to systemic inflammation. These findings prove that the prototype programming of IL-12 is abnormal in CSU.https://www.mdpi.com/2075-4426/14/3/295chronic urticariaIL-12p70IL-12p35IL-12p40inflammatory phenotypeanti-H1 therapy |
| spellingShingle | Corina Daniela Ene Milena Tocut Mircea Tampa Simona Roxana Georgescu Clara Matei Iulia Maria Teodora Leulescu Ilinca Nicolae Cosmin Ene Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria Journal of Personalized Medicine chronic urticaria IL-12p70 IL-12p35 IL-12p40 inflammatory phenotype anti-H1 therapy |
| title | Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria |
| title_full | Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria |
| title_fullStr | Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria |
| title_full_unstemmed | Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria |
| title_short | Changes in Serum IL-12 Levels following the Administration of H1-Antihistamines in Patients with Chronic Spontaneous Urticaria |
| title_sort | changes in serum il 12 levels following the administration of h1 antihistamines in patients with chronic spontaneous urticaria |
| topic | chronic urticaria IL-12p70 IL-12p35 IL-12p40 inflammatory phenotype anti-H1 therapy |
| url | https://www.mdpi.com/2075-4426/14/3/295 |
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