MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes
Recent research has revealed the importance of miRNAs in the diagnosis and clinical evolution of papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between specific subtypes of PTC. Methods: In this cross-sectional study, total RNA was extracted from...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/136 |
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| author | Horatiu Silaghi Laura Ancuța Pop Carmen Emanuela Georgescu Diana Muntean Doinița Crișan Patricia Silaghi Ionela Lungu Bogdana Adriana Nasui Eva-H. Dulf Cornelia Braicu Ioana Berindan-Neagoe Cristina Alina Silaghi |
| author_facet | Horatiu Silaghi Laura Ancuța Pop Carmen Emanuela Georgescu Diana Muntean Doinița Crișan Patricia Silaghi Ionela Lungu Bogdana Adriana Nasui Eva-H. Dulf Cornelia Braicu Ioana Berindan-Neagoe Cristina Alina Silaghi |
| author_sort | Horatiu Silaghi |
| collection | DOAJ |
| description | Recent research has revealed the importance of miRNAs in the diagnosis and clinical evolution of papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between specific subtypes of PTC. Methods: In this cross-sectional study, total RNA was extracted from paraffin-embedded tissues of 43 patients, 17 with an infiltrative follicular variant of PTC (iFVPTC) and 26 with a conventional variant of PTC (cPTC). Nine miRNAs were evaluated using qRT-PCR technology and specific miRNA assays. Results: We found specific patterns for cPTC and iFVPTC, such as miRNA altered in both types of tumours (miR-146b-5p, miR-181a-5p, miR-221-3p, miR-21-5p and miR-222-3p) and two miRNAs significantly expressed only in cPTC (miR-20b-5p, miR-21-5p). The iFVPTC group presented strong and moderate correlations between miRNA expression and clinical data. miR-221-3p, miR-195-5p, miR-181-5p, miR-146b-5p and miR-222 were correlated with age, tumour size (TS) or lymph node metastases (N), while only miR-20b-5p, miR-195-5p and miR-181-5p were correlated with TS, N and age in the cPTC group. Conclusions: The present study allowed the identification of a signature of two miRNAs to confirm miRNA differences between the two histological subtypes of TC. Our results provide advances in the molecular diagnosis of TC and could help to improve the diagnostic performance of already existing molecular classifiers. |
| first_indexed | 2024-03-08T09:57:20Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:20Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-ed2a8d50744d49888f2d8ce4100401862024-01-29T13:47:26ZengMDPI AGBiomedicines2227-90592024-01-0112113610.3390/biomedicines12010136MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma SubtypesHoratiu Silaghi0Laura Ancuța Pop1Carmen Emanuela Georgescu2Diana Muntean3Doinița Crișan4Patricia Silaghi5Ionela Lungu6Bogdana Adriana Nasui7Eva-H. Dulf8Cornelia Braicu9Ioana Berindan-Neagoe10Cristina Alina Silaghi11Department of Surgery V, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 8 Victor Babes Street, 400012 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 400337 Cluj-Napoca, RomaniaDepartment of Endocrinology, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 8 Victor Babes Street, 400012 Cluj-Napoca, RomaniaDepartment of Pathology, Clinic Municipal Hospital Cluj-Napoca, Tăbăcarilor Street 11, 400139 Cluj-Napoca, RomaniaDepartment of Pathology, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 6 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaFaculty of Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 6 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaCardiomed Medical Center, 17 Republicii Street, 400015 Cluj-Napoca, RomaniaDepartment of Community Health, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 6 Louis Pasteur Street, 400349 Cluj-Napoca, RomaniaDepartment of Automation, Faculty of Automation and Computer Science, Technical University of Cluj-Napoca, 28 Memorandumului Street, 400014 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 400337 Cluj-Napoca, RomaniaResearch Center for Functional Genomics, Biomedicine and Translational Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 400337 Cluj-Napoca, RomaniaDepartment of Endocrinology, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca, 8 Victor Babes Street, 400012 Cluj-Napoca, RomaniaRecent research has revealed the importance of miRNAs in the diagnosis and clinical evolution of papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between specific subtypes of PTC. Methods: In this cross-sectional study, total RNA was extracted from paraffin-embedded tissues of 43 patients, 17 with an infiltrative follicular variant of PTC (iFVPTC) and 26 with a conventional variant of PTC (cPTC). Nine miRNAs were evaluated using qRT-PCR technology and specific miRNA assays. Results: We found specific patterns for cPTC and iFVPTC, such as miRNA altered in both types of tumours (miR-146b-5p, miR-181a-5p, miR-221-3p, miR-21-5p and miR-222-3p) and two miRNAs significantly expressed only in cPTC (miR-20b-5p, miR-21-5p). The iFVPTC group presented strong and moderate correlations between miRNA expression and clinical data. miR-221-3p, miR-195-5p, miR-181-5p, miR-146b-5p and miR-222 were correlated with age, tumour size (TS) or lymph node metastases (N), while only miR-20b-5p, miR-195-5p and miR-181-5p were correlated with TS, N and age in the cPTC group. Conclusions: The present study allowed the identification of a signature of two miRNAs to confirm miRNA differences between the two histological subtypes of TC. Our results provide advances in the molecular diagnosis of TC and could help to improve the diagnostic performance of already existing molecular classifiers.https://www.mdpi.com/2227-9059/12/1/136biomarkersdiagnosismiRNAsthyroid cancerpapillary thyroid cancerfollicular variant of papillary thyroid cancer |
| spellingShingle | Horatiu Silaghi Laura Ancuța Pop Carmen Emanuela Georgescu Diana Muntean Doinița Crișan Patricia Silaghi Ionela Lungu Bogdana Adriana Nasui Eva-H. Dulf Cornelia Braicu Ioana Berindan-Neagoe Cristina Alina Silaghi MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes Biomedicines biomarkers diagnosis miRNAs thyroid cancer papillary thyroid cancer follicular variant of papillary thyroid cancer |
| title | MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes |
| title_full | MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes |
| title_fullStr | MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes |
| title_full_unstemmed | MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes |
| title_short | MicroRNA Expression Profiling—Potential Molecular Discrimination of Papillary Thyroid Carcinoma Subtypes |
| title_sort | microrna expression profiling potential molecular discrimination of papillary thyroid carcinoma subtypes |
| topic | biomarkers diagnosis miRNAs thyroid cancer papillary thyroid cancer follicular variant of papillary thyroid cancer |
| url | https://www.mdpi.com/2227-9059/12/1/136 |
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