The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats
Summary: Background: A high concentration of energy substrate in circulation will suppress appetite and subsequent food intake. Medium-chain triglycerides (MCT) is a natural exogenous ketone source that can act as an energy substrate, increase blood ketone and suppress appetite. MCT emulsification...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Clinical Nutrition Open Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667268521000218 |
| _version_ | 1818891981702561792 |
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| author | Hendy Wijaya Kuncoro Foe Ami Soewandhi Hevi Wihadmadyatami Yudy Tjahjono |
| author_facet | Hendy Wijaya Kuncoro Foe Ami Soewandhi Hevi Wihadmadyatami Yudy Tjahjono |
| author_sort | Hendy Wijaya |
| collection | DOAJ |
| description | Summary: Background: A high concentration of energy substrate in circulation will suppress appetite and subsequent food intake. Medium-chain triglycerides (MCT) is a natural exogenous ketone source that can act as an energy substrate, increase blood ketone and suppress appetite. MCT emulsification increases its ketogenic properties. Interestingly, Isomaltooligosaccharide (IMO), a form of dietary fiber, can absorb and retain water, induce the stomach wall distention, and therefore could induce the afferent signal that causes appetite suppression. Despite those facts, there are no data available on whether a food supplement contains a combination of IMO and emulsified MCT (FC-MCT) could induce more appetite suppression. Objectives: This research investigated the effect of FC-MCT on blood glucose, β-hydroxybutyrate (βHB), and calorie intake in male Wistar rats. Methods: A total of 31 male Wistar rats were divided into four groups. 1) The MC group (n = 8) - MCT 10 ml/kg of body weight; 2) the FC group (n = 8) - combination of IMO and emulsified coconut oil or FC-CO 100% (w/v) 15 ml/kg of body weight; 3) the FM group (n = 7) - FC-MCT 100% (w/v) 15 ml/kg of body weight; and 4) the SU group (n = 8) - sucrose solution 20% (w/v) 15 ml/kg of body weight. Emulsified coconut oil acted as an isocaloric adjustor for IMO to balance MCT. Blood glucose and βHB were observed for 4 h, with 1 (one) hour measurement interval, presented as the area under the curve (AUC). The food intake was measured 4 h post supplementation. Results: Compared with other groups, we observed low Blood glucose AUC (p = 0.000), total calorie (p = 0.000) and food intake (p = 0.000) and high βHB AUC (p = 0.000) in the FM groups. Conclusion: We could successfully demonstrate that FC-MCT supplementation before a meal could suppress appetite and calorie intake. The caloric suppression seems to be mediated through the increased blood βHB concentration. |
| first_indexed | 2024-12-19T17:49:27Z |
| format | Article |
| id | doaj.art-ed2e1b96fed74a4caf4566961dbac874 |
| institution | Directory Open Access Journal |
| issn | 2667-2685 |
| language | English |
| last_indexed | 2024-12-19T17:49:27Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Nutrition Open Science |
| spelling | doaj.art-ed2e1b96fed74a4caf4566961dbac8742022-12-21T20:11:57ZengElsevierClinical Nutrition Open Science2667-26852021-06-01373546The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar ratsHendy Wijaya0Kuncoro Foe1Ami Soewandhi2Hevi Wihadmadyatami3Yudy Tjahjono4Biomedical Laboratory, Faculty of Pharmacy, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan No.1, Kalisari, Mulyorejo, Surabaya, East Java, 60112, Indonesia; Corresponding author. Telp/HP. (+62)87861102341.Biomedical Laboratory, Faculty of Pharmacy, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan No.1, Kalisari, Mulyorejo, Surabaya, East Java, 60112, IndonesiaBiomedical Laboratory, Faculty of Pharmacy, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan No.1, Kalisari, Mulyorejo, Surabaya, East Java, 60112, IndonesiaFaculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna No.2, Sleman, 55281, Yogyakarta, IndonesiaBiomedical Laboratory, Faculty of Pharmacy, Universitas Katolik Widya Mandala Surabaya, Jl. Kalisari Selatan No.1, Kalisari, Mulyorejo, Surabaya, East Java, 60112, IndonesiaSummary: Background: A high concentration of energy substrate in circulation will suppress appetite and subsequent food intake. Medium-chain triglycerides (MCT) is a natural exogenous ketone source that can act as an energy substrate, increase blood ketone and suppress appetite. MCT emulsification increases its ketogenic properties. Interestingly, Isomaltooligosaccharide (IMO), a form of dietary fiber, can absorb and retain water, induce the stomach wall distention, and therefore could induce the afferent signal that causes appetite suppression. Despite those facts, there are no data available on whether a food supplement contains a combination of IMO and emulsified MCT (FC-MCT) could induce more appetite suppression. Objectives: This research investigated the effect of FC-MCT on blood glucose, β-hydroxybutyrate (βHB), and calorie intake in male Wistar rats. Methods: A total of 31 male Wistar rats were divided into four groups. 1) The MC group (n = 8) - MCT 10 ml/kg of body weight; 2) the FC group (n = 8) - combination of IMO and emulsified coconut oil or FC-CO 100% (w/v) 15 ml/kg of body weight; 3) the FM group (n = 7) - FC-MCT 100% (w/v) 15 ml/kg of body weight; and 4) the SU group (n = 8) - sucrose solution 20% (w/v) 15 ml/kg of body weight. Emulsified coconut oil acted as an isocaloric adjustor for IMO to balance MCT. Blood glucose and βHB were observed for 4 h, with 1 (one) hour measurement interval, presented as the area under the curve (AUC). The food intake was measured 4 h post supplementation. Results: Compared with other groups, we observed low Blood glucose AUC (p = 0.000), total calorie (p = 0.000) and food intake (p = 0.000) and high βHB AUC (p = 0.000) in the FM groups. Conclusion: We could successfully demonstrate that FC-MCT supplementation before a meal could suppress appetite and calorie intake. The caloric suppression seems to be mediated through the increased blood βHB concentration.http://www.sciencedirect.com/science/article/pii/S2667268521000218IsomaltooligosaccharidesMedium-chain triglyceridesAppetiteFood intakeObesity |
| spellingShingle | Hendy Wijaya Kuncoro Foe Ami Soewandhi Hevi Wihadmadyatami Yudy Tjahjono The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats Clinical Nutrition Open Science Isomaltooligosaccharides Medium-chain triglycerides Appetite Food intake Obesity |
| title | The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats |
| title_full | The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats |
| title_fullStr | The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats |
| title_full_unstemmed | The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats |
| title_short | The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, β-hydroxybutyrate and calorie intake in male Wistar rats |
| title_sort | effect of supplementation of isomaltooligosaccharide and emulsified medium chain triglycerides on blood glucose β hydroxybutyrate and calorie intake in male wistar rats |
| topic | Isomaltooligosaccharides Medium-chain triglycerides Appetite Food intake Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S2667268521000218 |
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