A portable system for economical nucleic acid amplification testing
Introduction: Regular and rapid large-scale screening for pathogens is crucial for controlling pandemics like Coronavirus Disease 2019 (COVID-19). In this study, we present the development of a digital point-of-care testing (POCT) system utilizing microfluidic paper-based analytical devices (μPADs)...
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| Format: | Article |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1214624/full |
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| author | Hui Dong Hui Dong Jin Mo Jin Mo Yongjian Yu Yongjian Yu Wantao Xie Wantao Xie Jianping Zheng Chao Jia Chao Jia |
| author_facet | Hui Dong Hui Dong Jin Mo Jin Mo Yongjian Yu Yongjian Yu Wantao Xie Wantao Xie Jianping Zheng Chao Jia Chao Jia |
| author_sort | Hui Dong |
| collection | DOAJ |
| description | Introduction: Regular and rapid large-scale screening for pathogens is crucial for controlling pandemics like Coronavirus Disease 2019 (COVID-19). In this study, we present the development of a digital point-of-care testing (POCT) system utilizing microfluidic paper-based analytical devices (μPADs) for the detection of SARS-CoV-2 gene fragments. The system incorporates temperature tuning and fluorescent detection components, along with intelligent and autonomous image acquisition and self-recognition programs.Methods: The developed POCT system is based on the nucleic acid amplification test (NAAT), a well-established molecular biology technique for detecting and amplifying nucleic acids. We successfully detected artificially synthesized SARS-CoV-2 gene fragments, namely ORF1ab gene, N gene, and E gene, with minimal reagent consumption of only 2.2 μL per readout, representing a mere 11% of the requirements of conventional in-tube methods. The power dissipation of the system was low, at 6.4 W.Results: Our testing results demonstrated that the proposed approach achieved a limit of detection of 1000 copies/mL, which is equivalent to detecting 1 copy or a single RNA template per reaction. By employing standard curve analysis, the quantity of the target templates can be accurately determined.Conclusion: The developed digital POCT system shows great promise for rapid and reliable detection of SARS-CoV-2 gene fragments, offering a cost-effective and efficient solution for controlling pandemics. Its compatibility with other diagnostic techniques and low reagent consumption make it a viable option to enhance healthcare in resource-limited areas. |
| first_indexed | 2024-03-12T17:59:55Z |
| format | Article |
| id | doaj.art-ed2e2877cde546dd9590354ca71fabd4 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-12T17:59:55Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ed2e2877cde546dd9590354ca71fabd42023-08-02T13:06:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-08-011110.3389/fbioe.2023.12146241214624A portable system for economical nucleic acid amplification testingHui Dong0Hui Dong1Jin Mo2Jin Mo3Yongjian Yu4Yongjian Yu5Wantao Xie6Wantao Xie7Jianping Zheng8Chao Jia9Chao Jia10School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, ChinaFujian Provincial Collaborative Innovation Center of High-End Equipment Manufacturing, Fuzhou, ChinaSchool of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, ChinaFujian Provincial Collaborative Innovation Center of High-End Equipment Manufacturing, Fuzhou, ChinaSchool of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, ChinaFujian Provincial Collaborative Innovation Center of High-End Equipment Manufacturing, Fuzhou, ChinaSchool of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, ChinaFujian Provincial Collaborative Innovation Center of High-End Equipment Manufacturing, Fuzhou, ChinaFujian Provincial Hospital, Fuzhou, Fujian, ChinaSchool of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, ChinaFujian Provincial Collaborative Innovation Center of High-End Equipment Manufacturing, Fuzhou, ChinaIntroduction: Regular and rapid large-scale screening for pathogens is crucial for controlling pandemics like Coronavirus Disease 2019 (COVID-19). In this study, we present the development of a digital point-of-care testing (POCT) system utilizing microfluidic paper-based analytical devices (μPADs) for the detection of SARS-CoV-2 gene fragments. The system incorporates temperature tuning and fluorescent detection components, along with intelligent and autonomous image acquisition and self-recognition programs.Methods: The developed POCT system is based on the nucleic acid amplification test (NAAT), a well-established molecular biology technique for detecting and amplifying nucleic acids. We successfully detected artificially synthesized SARS-CoV-2 gene fragments, namely ORF1ab gene, N gene, and E gene, with minimal reagent consumption of only 2.2 μL per readout, representing a mere 11% of the requirements of conventional in-tube methods. The power dissipation of the system was low, at 6.4 W.Results: Our testing results demonstrated that the proposed approach achieved a limit of detection of 1000 copies/mL, which is equivalent to detecting 1 copy or a single RNA template per reaction. By employing standard curve analysis, the quantity of the target templates can be accurately determined.Conclusion: The developed digital POCT system shows great promise for rapid and reliable detection of SARS-CoV-2 gene fragments, offering a cost-effective and efficient solution for controlling pandemics. Its compatibility with other diagnostic techniques and low reagent consumption make it a viable option to enhance healthcare in resource-limited areas.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1214624/fullpaper microfluidicsPOCTNAATSARS-CoV-2 template detectionautomatic image processing |
| spellingShingle | Hui Dong Hui Dong Jin Mo Jin Mo Yongjian Yu Yongjian Yu Wantao Xie Wantao Xie Jianping Zheng Chao Jia Chao Jia A portable system for economical nucleic acid amplification testing Frontiers in Bioengineering and Biotechnology paper microfluidics POCT NAAT SARS-CoV-2 template detection automatic image processing |
| title | A portable system for economical nucleic acid amplification testing |
| title_full | A portable system for economical nucleic acid amplification testing |
| title_fullStr | A portable system for economical nucleic acid amplification testing |
| title_full_unstemmed | A portable system for economical nucleic acid amplification testing |
| title_short | A portable system for economical nucleic acid amplification testing |
| title_sort | portable system for economical nucleic acid amplification testing |
| topic | paper microfluidics POCT NAAT SARS-CoV-2 template detection automatic image processing |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1214624/full |
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