Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells
Objective:: To investigat the mechanism of antitumor efficacy of Origanum dayi (O. dayi) and Ochradenus baccatus (O. baccatus) extracts by exploring apoptosis-inducing potential. Methods:: The aqueous extracts of aerial parts of aforementioned plants were prepared and used for this study. HepG2 cell...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-02-01
|
| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301507 |
| _version_ | 1811299965198139392 |
|---|---|
| author | Deepak Bhatia Animesh Mandal Eviatar Nevo Anupam Bishayee |
| author_facet | Deepak Bhatia Animesh Mandal Eviatar Nevo Anupam Bishayee |
| author_sort | Deepak Bhatia |
| collection | DOAJ |
| description | Objective:: To investigat the mechanism of antitumor efficacy of Origanum dayi (O. dayi) and Ochradenus baccatus (O. baccatus) extracts by exploring apoptosis-inducing potential.
Methods:: The aqueous extracts of aerial parts of aforementioned plants were prepared and used for this study. HepG2 cells were treated with varying concentrations (0, 2 and 5 mg/mL) of each plant extract for 24 or 48 h. Cell apoptosis was measured by annexin V-fluorescein isothiocyanate binding assay and flow cytometry. The expression levels of various apoptosis-related genes were determined by semi-quantitative reverse transcription-polymerase chain reaction.
Results:: O. dayi and O. baccatus extracts exerted apoptotic effects on HepG2 cells for 48 h following treatment. O. dayi extract was found to be a better apoptosis-inducing agent than O. baccatus extract as the former delivered greater efficacy at a lower concentration. Both extracts manifested upregulation of Bax, Bad, cytochrome c, caspase-3, caspase-7, caspase-9 and poly (adenosine diphosphate-ribose) polymerase.
Conclusions:: The aqueous extracts of O. dayi and O. baccatus are capable of inducing apoptosis in HepG2 cells through modulation of mitochondrial pathway which explains their antitumor activities. These desert plants may serve as useful resources to develop effective remedies for hepatocellular carcinoma and other human malignancies. |
| first_indexed | 2024-04-13T06:43:28Z |
| format | Article |
| id | doaj.art-ed31678b3d5b46d0b4de677281e00c88 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-04-13T06:43:28Z |
| publishDate | 2015-02-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-ed31678b3d5b46d0b4de677281e00c882022-12-22T02:57:40ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-02-0152879210.1016/S2221-1691(15)30150-7Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cellsDeepak Bhatia0Animesh Mandal1Eviatar Nevo2Anupam Bishayee3Department of Pharmacogenomics, Bernard J Dunn School of Pharmacy, Shenandoah University, Ashburn, VA 20147, USADepartment of Pharmaceutical Sciences, College of Pharmacy, Northeast Ohio Medical University, Rootstown, OH 44272, USAInstitute of Evolution and International Graduate Center of Evolution, University of Haifa, Mount Carmel, Haifa 31905, IsraelDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USAObjective:: To investigat the mechanism of antitumor efficacy of Origanum dayi (O. dayi) and Ochradenus baccatus (O. baccatus) extracts by exploring apoptosis-inducing potential. Methods:: The aqueous extracts of aerial parts of aforementioned plants were prepared and used for this study. HepG2 cells were treated with varying concentrations (0, 2 and 5 mg/mL) of each plant extract for 24 or 48 h. Cell apoptosis was measured by annexin V-fluorescein isothiocyanate binding assay and flow cytometry. The expression levels of various apoptosis-related genes were determined by semi-quantitative reverse transcription-polymerase chain reaction. Results:: O. dayi and O. baccatus extracts exerted apoptotic effects on HepG2 cells for 48 h following treatment. O. dayi extract was found to be a better apoptosis-inducing agent than O. baccatus extract as the former delivered greater efficacy at a lower concentration. Both extracts manifested upregulation of Bax, Bad, cytochrome c, caspase-3, caspase-7, caspase-9 and poly (adenosine diphosphate-ribose) polymerase. Conclusions:: The aqueous extracts of O. dayi and O. baccatus are capable of inducing apoptosis in HepG2 cells through modulation of mitochondrial pathway which explains their antitumor activities. These desert plants may serve as useful resources to develop effective remedies for hepatocellular carcinoma and other human malignancies.http://www.sciencedirect.com/science/article/pii/S2221169115301507Ochradenus baccatusOriganum dayiHepG2 cellsApoptosisGene expression |
| spellingShingle | Deepak Bhatia Animesh Mandal Eviatar Nevo Anupam Bishayee Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells Asian Pacific Journal of Tropical Biomedicine Ochradenus baccatus Origanum dayi HepG2 cells Apoptosis Gene expression |
| title | Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells |
| title_full | Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells |
| title_fullStr | Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells |
| title_full_unstemmed | Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells |
| title_short | Apoptosis-inducing effects of extracts from desert plants in HepG2 human hepatocarcinoma cells |
| title_sort | apoptosis inducing effects of extracts from desert plants in hepg2 human hepatocarcinoma cells |
| topic | Ochradenus baccatus Origanum dayi HepG2 cells Apoptosis Gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301507 |
| work_keys_str_mv | AT deepakbhatia apoptosisinducingeffectsofextractsfromdesertplantsinhepg2humanhepatocarcinomacells AT animeshmandal apoptosisinducingeffectsofextractsfromdesertplantsinhepg2humanhepatocarcinomacells AT eviatarnevo apoptosisinducingeffectsofextractsfromdesertplantsinhepg2humanhepatocarcinomacells AT anupambishayee apoptosisinducingeffectsofextractsfromdesertplantsinhepg2humanhepatocarcinomacells |