Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension.
Transforming growth factor beta (TGF-β) has been shown to play a critical role in pathogenesis of pulmonary arterial hypertension (PAH) although the precise role of TGF-β signaling remains uncertain. A recent report has shown that periostin (Pn) is one of the most upregulated proteins in human PAH l...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0220795 |
| _version_ | 1818581122729115648 |
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| author | Mitsuru Seki Nozomi Furukawa Norimichi Koitabashi Masaru Obokata Simon J Conway Hirokazu Arakawa Masahiko Kurabayashi |
| author_facet | Mitsuru Seki Nozomi Furukawa Norimichi Koitabashi Masaru Obokata Simon J Conway Hirokazu Arakawa Masahiko Kurabayashi |
| author_sort | Mitsuru Seki |
| collection | DOAJ |
| description | Transforming growth factor beta (TGF-β) has been shown to play a critical role in pathogenesis of pulmonary arterial hypertension (PAH) although the precise role of TGF-β signaling remains uncertain. A recent report has shown that periostin (Pn) is one of the most upregulated proteins in human PAH lung compared with healthy lungs. We established type I TGF-β receptor knockout mice specifically with Pn expressing cell (Pn-Cre/Tgfb1fl/fl mice). Increases in PA pressure and pulmonary artery muscularization were induced by hypoxia of 10% oxygen for 4 weeks. Lung Pn expression was markedly induced by 4 week-hypoxia. Pn-Cre/Tgfb1fl/fl mice showed lower right ventricular pressure elevation, inhibition of PA medial thickening. Fluorescent co-immunostaining showed that Smad3 activation in Pn expressing cell is attenuated. These results suggest that TGF-β signaling in Pn expressing cell may have an important role in the pathogenesis of PAH by controlling medial thickening. |
| first_indexed | 2024-12-16T07:28:28Z |
| format | Article |
| id | doaj.art-ed327ba5a2ac49868e25db0b09257e19 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T07:28:28Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ed327ba5a2ac49868e25db0b09257e192022-12-21T22:39:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01148e022079510.1371/journal.pone.0220795Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension.Mitsuru SekiNozomi FurukawaNorimichi KoitabashiMasaru ObokataSimon J ConwayHirokazu ArakawaMasahiko KurabayashiTransforming growth factor beta (TGF-β) has been shown to play a critical role in pathogenesis of pulmonary arterial hypertension (PAH) although the precise role of TGF-β signaling remains uncertain. A recent report has shown that periostin (Pn) is one of the most upregulated proteins in human PAH lung compared with healthy lungs. We established type I TGF-β receptor knockout mice specifically with Pn expressing cell (Pn-Cre/Tgfb1fl/fl mice). Increases in PA pressure and pulmonary artery muscularization were induced by hypoxia of 10% oxygen for 4 weeks. Lung Pn expression was markedly induced by 4 week-hypoxia. Pn-Cre/Tgfb1fl/fl mice showed lower right ventricular pressure elevation, inhibition of PA medial thickening. Fluorescent co-immunostaining showed that Smad3 activation in Pn expressing cell is attenuated. These results suggest that TGF-β signaling in Pn expressing cell may have an important role in the pathogenesis of PAH by controlling medial thickening.https://doi.org/10.1371/journal.pone.0220795 |
| spellingShingle | Mitsuru Seki Nozomi Furukawa Norimichi Koitabashi Masaru Obokata Simon J Conway Hirokazu Arakawa Masahiko Kurabayashi Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. PLoS ONE |
| title | Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. |
| title_full | Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. |
| title_fullStr | Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. |
| title_full_unstemmed | Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. |
| title_short | Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. |
| title_sort | periostin expressing cell specific transforming growth factor β inhibition in pulmonary artery prevents pulmonary arterial hypertension |
| url | https://doi.org/10.1371/journal.pone.0220795 |
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