Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts
Background and Objective: The increase of nosocomial systematic fungal infections due to pathogenic yeast, led to researchers on finding novel antifungals with potent inhibitory activity toward a wide range of pathogenic fungi. In the present study, antifungal effect of aqueous garlic extract indivi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Golestan University of Medical Sciences
2009-05-01
|
| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-346&slc_lang=en&sid=1 |
| _version_ | 1818519637973794816 |
|---|---|
| author | Atoosa Razzagh Parast (MSc) Masoomeh Shams Ghahfarokhi (PhD) Mohammad Hossein Yadegari (PhD) Mehdi Razzaghi Abyaneh (PhD) |
| author_facet | Atoosa Razzagh Parast (MSc) Masoomeh Shams Ghahfarokhi (PhD) Mohammad Hossein Yadegari (PhD) Mehdi Razzaghi Abyaneh (PhD) |
| author_sort | Atoosa Razzagh Parast (MSc) |
| collection | DOAJ |
| description | Background and Objective: The increase of nosocomial systematic fungal infections due to pathogenic yeast, led to researchers on finding novel antifungals with potent inhibitory activity toward a wide range of pathogenic fungi. In the present study, antifungal effect of aqueous garlic extract individually and in combination with Fluconazole, Itraconazole and Ketoconazole were studied against some pathogenic yeasts. Materials and Methods: Broth microdilution method was used for evaluating antifungal activities of aqueous garlic extract with 0.03-256 µg/ml individually and in combination with Fluconazole, Itraconazole and Ketoconazole against Candida albicans PTCC5057, Candida dubliniensis CD36, Cryptococcus neoformance CNE1 and Malassezia furfur MF1, in vitro. The microdilution method was used for assessing antifungal susceptibility of above-mentioned compounds in two culture media sabouraud dextrose broth (for all fungi except M.furfur) and modified Dixon broth (for only M.furfur). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of aqueous garlic extract and antifungal drugs tested were determined by on comparison of colony forming units (CFU) between test and control groups. Results: Aqueous garlic extract inhibited the growth of all fungi tested in a dose-dependent manner, in a concentration comparable with azole drugs.The MIC ranges of aqueous garlic extract, Candida albicans, Candida dubliniensis, Cryptococcus neoformances and Malassezia furfur was determined to be 0.25-64 g/ml. The MIC ranges of aqueous garlic extract in combination with Fluconazole was determined 0.125-8, 0.25-16, 0.125-16 and 0.5-8 µg/ml, respectively. The MIC ranges of aqueous garlic extract in combination with Itraconazole was determined 0.25-8, 0.125-2, 0.125-16, 0.25-4 µg/ml, respectively.The MIC ranges of aqueous garlic extract in combination with Ketoconazole was determined 0.125-4, 0.125-1, 0.125-8 and 0.125-2 µg/ml, respectively.The results indicated that the antifungal activities of drugs were increased in combination with aqueous garlic extract (P<0.05). Conclusion: This study showed that, the aqueous garlic extract increased the antifungal activity and decreased MIC of drugs in combination with them. |
| first_indexed | 2024-12-11T01:26:49Z |
| format | Article |
| id | doaj.art-ed35fb9cf55f41b8ab015687f2c7a050 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-12-11T01:26:49Z |
| publishDate | 2009-05-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-ed35fb9cf55f41b8ab015687f2c7a0502022-12-22T01:25:30ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802009-05-011114956Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeastsAtoosa Razzagh Parast (MSc)0Masoomeh Shams Ghahfarokhi (PhD)1Mohammad Hossein Yadegari (PhD)2Mehdi Razzaghi Abyaneh (PhD)3 Background and Objective: The increase of nosocomial systematic fungal infections due to pathogenic yeast, led to researchers on finding novel antifungals with potent inhibitory activity toward a wide range of pathogenic fungi. In the present study, antifungal effect of aqueous garlic extract individually and in combination with Fluconazole, Itraconazole and Ketoconazole were studied against some pathogenic yeasts. Materials and Methods: Broth microdilution method was used for evaluating antifungal activities of aqueous garlic extract with 0.03-256 µg/ml individually and in combination with Fluconazole, Itraconazole and Ketoconazole against Candida albicans PTCC5057, Candida dubliniensis CD36, Cryptococcus neoformance CNE1 and Malassezia furfur MF1, in vitro. The microdilution method was used for assessing antifungal susceptibility of above-mentioned compounds in two culture media sabouraud dextrose broth (for all fungi except M.furfur) and modified Dixon broth (for only M.furfur). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of aqueous garlic extract and antifungal drugs tested were determined by on comparison of colony forming units (CFU) between test and control groups. Results: Aqueous garlic extract inhibited the growth of all fungi tested in a dose-dependent manner, in a concentration comparable with azole drugs.The MIC ranges of aqueous garlic extract, Candida albicans, Candida dubliniensis, Cryptococcus neoformances and Malassezia furfur was determined to be 0.25-64 g/ml. The MIC ranges of aqueous garlic extract in combination with Fluconazole was determined 0.125-8, 0.25-16, 0.125-16 and 0.5-8 µg/ml, respectively. The MIC ranges of aqueous garlic extract in combination with Itraconazole was determined 0.25-8, 0.125-2, 0.125-16, 0.25-4 µg/ml, respectively.The MIC ranges of aqueous garlic extract in combination with Ketoconazole was determined 0.125-4, 0.125-1, 0.125-8 and 0.125-2 µg/ml, respectively.The results indicated that the antifungal activities of drugs were increased in combination with aqueous garlic extract (P<0.05). Conclusion: This study showed that, the aqueous garlic extract increased the antifungal activity and decreased MIC of drugs in combination with them.http://goums.ac.ir/journal/browse.php?a_code=A-10-1-346&slc_lang=en&sid=1Pathogenic yeastsAllium sativumFluconazoleItraconazoleKetoconazoleSynergistic effects |
| spellingShingle | Atoosa Razzagh Parast (MSc) Masoomeh Shams Ghahfarokhi (PhD) Mohammad Hossein Yadegari (PhD) Mehdi Razzaghi Abyaneh (PhD) Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts مجله دانشگاه علوم پزشکی گرگان Pathogenic yeasts Allium sativum Fluconazole Itraconazole Ketoconazole Synergistic effects |
| title | Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts |
| title_full | Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts |
| title_fullStr | Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts |
| title_full_unstemmed | Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts |
| title_short | Antifungal effect of Allium sativum either individually or in combination with Fluconazole, Itraconazole and Ketoconazole against pathogenic yeasts |
| title_sort | antifungal effect of allium sativum either individually or in combination with fluconazole itraconazole and ketoconazole against pathogenic yeasts |
| topic | Pathogenic yeasts Allium sativum Fluconazole Itraconazole Ketoconazole Synergistic effects |
| url | http://goums.ac.ir/journal/browse.php?a_code=A-10-1-346&slc_lang=en&sid=1 |
| work_keys_str_mv | AT atoosarazzaghparastmsc antifungaleffectofalliumsativumeitherindividuallyorincombinationwithfluconazoleitraconazoleandketoconazoleagainstpathogenicyeasts AT masoomehshamsghahfarokhiphd antifungaleffectofalliumsativumeitherindividuallyorincombinationwithfluconazoleitraconazoleandketoconazoleagainstpathogenicyeasts AT mohammadhosseinyadegariphd antifungaleffectofalliumsativumeitherindividuallyorincombinationwithfluconazoleitraconazoleandketoconazoleagainstpathogenicyeasts AT mehdirazzaghiabyanehphd antifungaleffectofalliumsativumeitherindividuallyorincombinationwithfluconazoleitraconazoleandketoconazoleagainstpathogenicyeasts |