Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing
Abstract Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | Experimental Hematology & Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40164-023-00391-5 |
| _version_ | 1797865365826437120 |
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| author | Wenqi Wu Zeyan Shi Zhongyuan Tang Huiqun Li Xiaoke Huang Xiaolin Liang Jing Li Yibin Yao Weihua Zhao Meiqing Wu Jun Luo Zhenfang Liu |
| author_facet | Wenqi Wu Zeyan Shi Zhongyuan Tang Huiqun Li Xiaoke Huang Xiaolin Liang Jing Li Yibin Yao Weihua Zhao Meiqing Wu Jun Luo Zhenfang Liu |
| author_sort | Wenqi Wu |
| collection | DOAJ |
| description | Abstract Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies. |
| first_indexed | 2024-04-09T23:07:59Z |
| format | Article |
| id | doaj.art-ed386b3963bd4341bef93eade0934ebe |
| institution | Directory Open Access Journal |
| issn | 2162-3619 |
| language | English |
| last_indexed | 2024-04-09T23:07:59Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Experimental Hematology & Oncology |
| spelling | doaj.art-ed386b3963bd4341bef93eade0934ebe2023-03-22T10:37:58ZengBMCExperimental Hematology & Oncology2162-36192023-03-0112111610.1186/s40164-023-00391-5Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencingWenqi Wu0Zeyan Shi1Zhongyuan Tang2Huiqun Li3Xiaoke Huang4Xiaolin Liang5Jing Li6Yibin Yao7Weihua Zhao8Meiqing Wu9Jun Luo10Zhenfang Liu11Department of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies.https://doi.org/10.1186/s40164-023-00391-5Single-cell RNA sequencingNormal karyotype acute myeloid leukemiaHeterogeneityLeukemia stem cell |
| spellingShingle | Wenqi Wu Zeyan Shi Zhongyuan Tang Huiqun Li Xiaoke Huang Xiaolin Liang Jing Li Yibin Yao Weihua Zhao Meiqing Wu Jun Luo Zhenfang Liu Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing Experimental Hematology & Oncology Single-cell RNA sequencing Normal karyotype acute myeloid leukemia Heterogeneity Leukemia stem cell |
| title | Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing |
| title_full | Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing |
| title_fullStr | Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing |
| title_full_unstemmed | Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing |
| title_short | Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing |
| title_sort | characterization of bone marrow heterogeneity in nk aml m4 m5 based on single cell rna sequencing |
| topic | Single-cell RNA sequencing Normal karyotype acute myeloid leukemia Heterogeneity Leukemia stem cell |
| url | https://doi.org/10.1186/s40164-023-00391-5 |
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