Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>)
Post-thawed sperm quality parameters vary across different species after cryopreservation. To date, the molecular mechanism of sperm cryoinjury, freeze-tolerance and other influential factors are largely unknown. In this study, significantly dysregulated microRNAs (miRNAs) and mRNAs in boar and gian...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/9/432 |
| _version_ | 1811262205671243776 |
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| author | Ming-Xia Ran Ying-Min Zhou Kai Liang Wen-Can Wang Yan Zhang Ming Zhang Jian-Dong Yang Guang-Bin Zhou Kai Wu Cheng-Dong Wang Yan Huang Bo Luo Izhar Hyder Qazi He-Min Zhang Chang-Jun Zeng |
| author_facet | Ming-Xia Ran Ying-Min Zhou Kai Liang Wen-Can Wang Yan Zhang Ming Zhang Jian-Dong Yang Guang-Bin Zhou Kai Wu Cheng-Dong Wang Yan Huang Bo Luo Izhar Hyder Qazi He-Min Zhang Chang-Jun Zeng |
| author_sort | Ming-Xia Ran |
| collection | DOAJ |
| description | Post-thawed sperm quality parameters vary across different species after cryopreservation. To date, the molecular mechanism of sperm cryoinjury, freeze-tolerance and other influential factors are largely unknown. In this study, significantly dysregulated microRNAs (miRNAs) and mRNAs in boar and giant panda sperm with different cryo-resistance capacity were evaluated. From the result of miRNA profile of fresh and frozen-thawed giant panda sperm, a total of 899 mature, novel miRNAs were identified, and 284 miRNAs were found to be significantly dysregulated (195 up-regulated and 89 down-regulated). Combined analysis of miRNA profiling of giant panda sperm and our previously published data on boar sperm, 46, 21 and 4 differentially expressed (DE) mRNAs in boar sperm were believed to be related to apoptosis, glycolysis and oxidative phosphorylation, respectively. Meanwhile, 87, 17 and 7 DE mRNAs in giant panda were associated with apoptosis, glycolysis and oxidative phosphorylation, respectively. Gene ontology (GO) analysis of the targets of DE miRNAs showed that they were mainly distributed on membrane related pathway in giant panda sperm, while cell components and cell processes were tied to the targets of DE miRNAs in boar sperm. Finally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DE mRNAs indicated that most of these DE mRNAs were distributed in membrane signal transduction-related pathways in giant panda sperm, while those in boar sperm were mainly distributed in the cytokine-cytokine receptor interaction pathway and inflammatory related pathways. In conclusion, although the different freezing extenders and programs were used, the DE miRNAs and mRNAs involved in apoptosis, energy metabolism, olfactory transduction pathway, inflammatory response and cytokine-cytokine interactions, could be the possible molecular mechanism of sperm cryoinjury and freeze tolerance. |
| first_indexed | 2024-04-12T19:21:04Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-12T19:21:04Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ed3b429696844eccb1d16d287cedf69d2022-12-22T03:19:36ZengMDPI AGBiomolecules2218-273X2019-09-019943210.3390/biom9090432biom9090432Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>)Ming-Xia Ran0Ying-Min Zhou1Kai Liang2Wen-Can Wang3Yan Zhang4Ming Zhang5Jian-Dong Yang6Guang-Bin Zhou7Kai Wu8Cheng-Dong Wang9Yan Huang10Bo Luo11Izhar Hyder Qazi12He-Min Zhang13Chang-Jun Zeng14College of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaChina Conservation and Research Center for the Giant Panda, Wolong 473000, ChinaCollege of Animal Sciences and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaPost-thawed sperm quality parameters vary across different species after cryopreservation. To date, the molecular mechanism of sperm cryoinjury, freeze-tolerance and other influential factors are largely unknown. In this study, significantly dysregulated microRNAs (miRNAs) and mRNAs in boar and giant panda sperm with different cryo-resistance capacity were evaluated. From the result of miRNA profile of fresh and frozen-thawed giant panda sperm, a total of 899 mature, novel miRNAs were identified, and 284 miRNAs were found to be significantly dysregulated (195 up-regulated and 89 down-regulated). Combined analysis of miRNA profiling of giant panda sperm and our previously published data on boar sperm, 46, 21 and 4 differentially expressed (DE) mRNAs in boar sperm were believed to be related to apoptosis, glycolysis and oxidative phosphorylation, respectively. Meanwhile, 87, 17 and 7 DE mRNAs in giant panda were associated with apoptosis, glycolysis and oxidative phosphorylation, respectively. Gene ontology (GO) analysis of the targets of DE miRNAs showed that they were mainly distributed on membrane related pathway in giant panda sperm, while cell components and cell processes were tied to the targets of DE miRNAs in boar sperm. Finally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of DE mRNAs indicated that most of these DE mRNAs were distributed in membrane signal transduction-related pathways in giant panda sperm, while those in boar sperm were mainly distributed in the cytokine-cytokine receptor interaction pathway and inflammatory related pathways. In conclusion, although the different freezing extenders and programs were used, the DE miRNAs and mRNAs involved in apoptosis, energy metabolism, olfactory transduction pathway, inflammatory response and cytokine-cytokine interactions, could be the possible molecular mechanism of sperm cryoinjury and freeze tolerance.https://www.mdpi.com/2218-273X/9/9/432spermcryoinjuryfreeze toleranceboargiant panda |
| spellingShingle | Ming-Xia Ran Ying-Min Zhou Kai Liang Wen-Can Wang Yan Zhang Ming Zhang Jian-Dong Yang Guang-Bin Zhou Kai Wu Cheng-Dong Wang Yan Huang Bo Luo Izhar Hyder Qazi He-Min Zhang Chang-Jun Zeng Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) Biomolecules sperm cryoinjury freeze tolerance boar giant panda |
| title | Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) |
| title_full | Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) |
| title_fullStr | Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) |
| title_full_unstemmed | Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) |
| title_short | Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (<i>Sus</i> <i>scrofa</i>) and Giant Panda (<i>Ailuropoda</i> <i>melanoleuca</i>) |
| title_sort | comparative analysis of microrna and mrna profiles of sperm with different freeze tolerance capacities in boar i sus i i scrofa i and giant panda i ailuropoda i i melanoleuca i |
| topic | sperm cryoinjury freeze tolerance boar giant panda |
| url | https://www.mdpi.com/2218-273X/9/9/432 |
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