Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study
Abstract Background Little is known about potential differences in the left recurrent laryngeal nerve (Lrln) and left cricoarytenoideus dorsalis (LCAD) muscle between domestic and feral horse populations. If a difference exists, feral horses may provide a useful control population for research relat...
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1186 |
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| author | Natasha E. Lean Samantha H. Franklin Cate Steel Lucy Woolford Jason White Benjamin J. Ahern |
| author_facet | Natasha E. Lean Samantha H. Franklin Cate Steel Lucy Woolford Jason White Benjamin J. Ahern |
| author_sort | Natasha E. Lean |
| collection | DOAJ |
| description | Abstract Background Little is known about potential differences in the left recurrent laryngeal nerve (Lrln) and left cricoarytenoideus dorsalis (LCAD) muscle between domestic and feral horse populations. If a difference exists, feral horses may provide a useful control population for research related to recurrent laryngeal neuropathy (RLN) and increase our understanding of potential population pressures influencing the incidence RLN. Objectives The objective of this study was to compare the Lrln and LCAD of domestic and feral horses using histological and immunohistochemical techniques (IHC). Methods Sixteen horses, domestic (n = 8) and feral (n = 8), without clinical or ancillary examinations that were processed at an abattoir had the Lrln and LCAD muscle harvested immediately following death. Carcass weights were recorded. Subjective and morphometric histologic assessment were performed on Lrln sections. The LCAD was assessed for myosin heavy chain (fibre type proportion, diameter and grouping using IHC. Results Fibre‐type grouping consistent with RLN was seen in both groups. Regenerating fibre clusters were more common in domestic compared to feral horses (p = 0.04). No other histologic differences occurred between groups. Muscle fibre typing demonstrated a lower mean percentage of type IIX fibres in the feral group compared to the domestic group (p = 0.03). There was no difference in type I or IIA proportions or mean diameter of any fibre type between the groups. Conclusions The domestic population showed evidence of nerve regeneration suggesting RLN in this group, yet this was not supported by the higher proportion of type IIX muscle fibres compared to the feral population. Further evaluation to clarify the significance and wider occurrence of the differences is indicated. |
| first_indexed | 2024-03-12T22:52:33Z |
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| id | doaj.art-ed401eda98ab4a749cd11faf59b4f6f1 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T22:52:33Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-ed401eda98ab4a749cd11faf59b4f6f12023-07-20T08:09:46ZengWileyVeterinary Medicine and Science2053-10952023-07-01941610161710.1002/vms3.1186Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot studyNatasha E. Lean0Samantha H. Franklin1Cate Steel2Lucy Woolford3Jason White4Benjamin J. Ahern5Equine Specialist Hospital School of Veterinary Science, University of Queensland, UQ Gatton Campus GattonQueenslandAustraliaEquine Health and Performance Centre University of Adelaide RoseworthySouth AustraliaAustraliaU‐Vet Equine Centre University of Melbourne WerribeeVictoriaAustraliaEquine Health and Performance Centre University of Adelaide RoseworthySouth AustraliaAustraliaOffice of the Pro Vice Chancellor Research and Innovation Charles Sturt University Wagga WaggaNew South WalesAustraliaEquine Specialist Hospital School of Veterinary Science, University of Queensland, UQ Gatton Campus GattonQueenslandAustraliaAbstract Background Little is known about potential differences in the left recurrent laryngeal nerve (Lrln) and left cricoarytenoideus dorsalis (LCAD) muscle between domestic and feral horse populations. If a difference exists, feral horses may provide a useful control population for research related to recurrent laryngeal neuropathy (RLN) and increase our understanding of potential population pressures influencing the incidence RLN. Objectives The objective of this study was to compare the Lrln and LCAD of domestic and feral horses using histological and immunohistochemical techniques (IHC). Methods Sixteen horses, domestic (n = 8) and feral (n = 8), without clinical or ancillary examinations that were processed at an abattoir had the Lrln and LCAD muscle harvested immediately following death. Carcass weights were recorded. Subjective and morphometric histologic assessment were performed on Lrln sections. The LCAD was assessed for myosin heavy chain (fibre type proportion, diameter and grouping using IHC. Results Fibre‐type grouping consistent with RLN was seen in both groups. Regenerating fibre clusters were more common in domestic compared to feral horses (p = 0.04). No other histologic differences occurred between groups. Muscle fibre typing demonstrated a lower mean percentage of type IIX fibres in the feral group compared to the domestic group (p = 0.03). There was no difference in type I or IIA proportions or mean diameter of any fibre type between the groups. Conclusions The domestic population showed evidence of nerve regeneration suggesting RLN in this group, yet this was not supported by the higher proportion of type IIX muscle fibres compared to the feral population. Further evaluation to clarify the significance and wider occurrence of the differences is indicated.https://doi.org/10.1002/vms3.1186cricoarytenoideus dorsalis musclehorselaryngeal hemiplegianeuropathyrecurrent laryngeal nerve |
| spellingShingle | Natasha E. Lean Samantha H. Franklin Cate Steel Lucy Woolford Jason White Benjamin J. Ahern Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study Veterinary Medicine and Science cricoarytenoideus dorsalis muscle horse laryngeal hemiplegia neuropathy recurrent laryngeal nerve |
| title | Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study |
| title_full | Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study |
| title_fullStr | Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study |
| title_full_unstemmed | Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study |
| title_short | Evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in Australia using histologic and immunohistochemical analysis: A pilot study |
| title_sort | evaluation of recurrent laryngeal neuropathy in domestic and feral horse populations in australia using histologic and immunohistochemical analysis a pilot study |
| topic | cricoarytenoideus dorsalis muscle horse laryngeal hemiplegia neuropathy recurrent laryngeal nerve |
| url | https://doi.org/10.1002/vms3.1186 |
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