Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon.
Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subu...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0275013 |
| _version_ | 1811345338029572096 |
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| author | Madeleine Eunice Betouke Ongwe Yoanne D Mouwenda Koen A Stam Peter G Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E Bottazzi Peter J Hotez Remko V Leeuwen Martin P Grobusch Ayola A Adegnika Oleg A Mayboroda Maria Yazdanbakhsh |
| author_facet | Madeleine Eunice Betouke Ongwe Yoanne D Mouwenda Koen A Stam Peter G Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E Bottazzi Peter J Hotez Remko V Leeuwen Martin P Grobusch Ayola A Adegnika Oleg A Mayboroda Maria Yazdanbakhsh |
| author_sort | Madeleine Eunice Betouke Ongwe |
| collection | DOAJ |
| description | Metabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity. |
| first_indexed | 2024-04-13T20:02:37Z |
| format | Article |
| id | doaj.art-ed445e508fab47d0a4352a9fcd870d4f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T20:02:37Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ed445e508fab47d0a4352a9fcd870d4f2022-12-22T02:32:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179e027501310.1371/journal.pone.0275013Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon.Madeleine Eunice Betouke OngweYoanne D MouwendaKoen A StamPeter G KremsnerBertrand LellDavid DiemertJeff BethonyMaria E BottazziPeter J HotezRemko V LeeuwenMartin P GrobuschAyola A AdegnikaOleg A MayborodaMaria YazdanbakhshMetabolomics provides a powerful tool to study physiological changes in response to various perturbations such as vaccination. We explored whether metabolomic changes could be seen after vaccination in a phase I trial where Gabonese adults living either in rural or semi-urban areas received the subunit hookworm vaccine candidates (Na-GST-1 and Na-APR-1 (M74) adjuvanted with Alhydrogel plus GLA-AF (n = 24) or the hepatitis B vaccine (n = 8) as control. Urine samples were collected and assayed using targeted 1H NMR spectroscopy. At baseline, a set of metabolites significantly distinguished rural from semi-urban individuals. The pre- and post-vaccination comparisons indicated significant changes in few metabolites but only one day after the first vaccination. There was no relationship with immunogenicity. In conclusion, in a small phase 1 trial, urinary metabolomics could distinguish volunteers with different environmental exposures and reflected the safety of the vaccines but did not show a relationship to immunogenicity.https://doi.org/10.1371/journal.pone.0275013 |
| spellingShingle | Madeleine Eunice Betouke Ongwe Yoanne D Mouwenda Koen A Stam Peter G Kremsner Bertrand Lell David Diemert Jeff Bethony Maria E Bottazzi Peter J Hotez Remko V Leeuwen Martin P Grobusch Ayola A Adegnika Oleg A Mayboroda Maria Yazdanbakhsh Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. PLoS ONE |
| title | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. |
| title_full | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. |
| title_fullStr | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. |
| title_full_unstemmed | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. |
| title_short | Investigation of urinary metabolomics in a phase I hookworm vaccine trial in Gabon. |
| title_sort | investigation of urinary metabolomics in a phase i hookworm vaccine trial in gabon |
| url | https://doi.org/10.1371/journal.pone.0275013 |
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