An updated protocol for in vitro bovine embryo production
Summary: Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol featu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722008048 |
| _version_ | 1811177651729072128 |
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| author | Savannah L. Speckhart Lydia K. Wooldridge Alan D. Ealy |
| author_facet | Savannah L. Speckhart Lydia K. Wooldridge Alan D. Ealy |
| author_sort | Savannah L. Speckhart |
| collection | DOAJ |
| description | Summary: Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively.For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T06:04:47Z |
| format | Article |
| id | doaj.art-ed4818f5b1fa4702bb268afdf938f3fb |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T06:04:47Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ed4818f5b1fa4702bb268afdf938f3fb2022-12-22T04:41:32ZengElsevierSTAR Protocols2666-16672023-03-0141101924An updated protocol for in vitro bovine embryo productionSavannah L. Speckhart0Lydia K. Wooldridge1Alan D. Ealy2School of Animal Sciences, Blacksburg, VA 24060, USA; Corresponding authorSchool of Animal Sciences, Blacksburg, VA 24060, USASchool of Animal Sciences, Blacksburg, VA 24060, USASummary: Cattle embryos represent a useful model for understanding parts of human embryogenesis due to various biological similarities. We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer. A thorough troubleshooting section will help users optimize embryo development with cleavage and blastocyst rates exceeding 70% and 20%, respectively.For complete details on the use and execution of this protocol, please refer to Wooldridge and Ealy (2019).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722008048Cell cultureDevelopmental biologyModel Organisms |
| spellingShingle | Savannah L. Speckhart Lydia K. Wooldridge Alan D. Ealy An updated protocol for in vitro bovine embryo production STAR Protocols Cell culture Developmental biology Model Organisms |
| title | An updated protocol for in vitro bovine embryo production |
| title_full | An updated protocol for in vitro bovine embryo production |
| title_fullStr | An updated protocol for in vitro bovine embryo production |
| title_full_unstemmed | An updated protocol for in vitro bovine embryo production |
| title_short | An updated protocol for in vitro bovine embryo production |
| title_sort | updated protocol for in vitro bovine embryo production |
| topic | Cell culture Developmental biology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722008048 |
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