The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset
Sudden sensorineural hearing loss (SSNHL) is an acquired idiopathic hearing loss. Serum levels of small, non-coding RNAs and microRNAs (miRNAs) miR-195-5p/-132-3p/-30a-3p/-128-3p/-140-3p/-186-5p/-375-3p/-590-5p are differentially expressed in SSNHL patients within 28 days of hearing loss onset. This...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/8/7307 |
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| author | Reyhaneh Abgoon Printha Wijesinghe Cathie Garnis Desmond A. Nunez |
| author_facet | Reyhaneh Abgoon Printha Wijesinghe Cathie Garnis Desmond A. Nunez |
| author_sort | Reyhaneh Abgoon |
| collection | DOAJ |
| description | Sudden sensorineural hearing loss (SSNHL) is an acquired idiopathic hearing loss. Serum levels of small, non-coding RNAs and microRNAs (miRNAs) miR-195-5p/-132-3p/-30a-3p/-128-3p/-140-3p/-186-5p/-375-3p/-590-5p are differentially expressed in SSNHL patients within 28 days of hearing loss onset. This study determines if these changes persist by comparing the serum miRNA expression profile of SSNHL patients within 1 month of hearing loss onset with that of patients 3–12 months after hearing loss onset. We collected serum from consenting adult SSNHL patients at presentation or during clinic follow-up. We matched patient samples drawn 3–12 months after hearing loss onset (delayed group, <i>n</i> = 9 patients) by age and sex to samples drawn from patients within 28 days of hearing loss onset (immediate group, <i>n</i> = 14 patients). We compared the real-time PCR-determined expression levels of the target miRNAs between the two groups. We calculated the air conduction pure-tone-averaged (PTA) audiometric thresholds in affected ears at the initial and final follow-up visits. We undertook inter-group comparisons of hearing outcome status and initial and final PTA audiometric thresholds. There was no significant inter-group difference in miRNA expression level, hearing recovery status and initial and final affected ear PTA audiometric thresholds. |
| first_indexed | 2024-03-11T04:56:36Z |
| format | Article |
| id | doaj.art-ed48275b91ea4ee9851b4d00cdc79655 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:56:36Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ed48275b91ea4ee9851b4d00cdc796552023-11-17T19:38:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248730710.3390/ijms24087307The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss OnsetReyhaneh Abgoon0Printha Wijesinghe1Cathie Garnis2Desmond A. Nunez3Division of Otolaryngology, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1M9, CanadaDivision of Otolaryngology, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1M9, CanadaDivision of Otolaryngology, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1M9, CanadaDivision of Otolaryngology, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1M9, CanadaSudden sensorineural hearing loss (SSNHL) is an acquired idiopathic hearing loss. Serum levels of small, non-coding RNAs and microRNAs (miRNAs) miR-195-5p/-132-3p/-30a-3p/-128-3p/-140-3p/-186-5p/-375-3p/-590-5p are differentially expressed in SSNHL patients within 28 days of hearing loss onset. This study determines if these changes persist by comparing the serum miRNA expression profile of SSNHL patients within 1 month of hearing loss onset with that of patients 3–12 months after hearing loss onset. We collected serum from consenting adult SSNHL patients at presentation or during clinic follow-up. We matched patient samples drawn 3–12 months after hearing loss onset (delayed group, <i>n</i> = 9 patients) by age and sex to samples drawn from patients within 28 days of hearing loss onset (immediate group, <i>n</i> = 14 patients). We compared the real-time PCR-determined expression levels of the target miRNAs between the two groups. We calculated the air conduction pure-tone-averaged (PTA) audiometric thresholds in affected ears at the initial and final follow-up visits. We undertook inter-group comparisons of hearing outcome status and initial and final PTA audiometric thresholds. There was no significant inter-group difference in miRNA expression level, hearing recovery status and initial and final affected ear PTA audiometric thresholds.https://www.mdpi.com/1422-0067/24/8/7307sudden sensorineural hearing lossmicroRNAsserum |
| spellingShingle | Reyhaneh Abgoon Printha Wijesinghe Cathie Garnis Desmond A. Nunez The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset International Journal of Molecular Sciences sudden sensorineural hearing loss microRNAs serum |
| title | The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset |
| title_full | The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset |
| title_fullStr | The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset |
| title_full_unstemmed | The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset |
| title_short | The Expression Levels of MicroRNAs Differentially Expressed in Sudden Sensorineural Hearing Loss Patients’ Serum Are Unchanged for up to 12 Months after Hearing Loss Onset |
| title_sort | expression levels of micrornas differentially expressed in sudden sensorineural hearing loss patients serum are unchanged for up to 12 months after hearing loss onset |
| topic | sudden sensorineural hearing loss microRNAs serum |
| url | https://www.mdpi.com/1422-0067/24/8/7307 |
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