A mechanistic protrusive-based model for 3D cell migration
Cell migration is essential for a variety of biological processes, such as embryogenesis, wound healing, and the immune response. After more than a century of research—mainly on flat surfaces—, there are still many unknowns about cell motility. In particular, regarding how cells migrate within 3D ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000589 |
| _version_ | 1818172869891325952 |
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| author | Francisco Merino-Casallo Maria Jose Gomez-Benito Ruben Martinez-Cantin Jose Manuel Garcia-Aznar |
| author_facet | Francisco Merino-Casallo Maria Jose Gomez-Benito Ruben Martinez-Cantin Jose Manuel Garcia-Aznar |
| author_sort | Francisco Merino-Casallo |
| collection | DOAJ |
| description | Cell migration is essential for a variety of biological processes, such as embryogenesis, wound healing, and the immune response. After more than a century of research—mainly on flat surfaces—, there are still many unknowns about cell motility. In particular, regarding how cells migrate within 3D matrices, which more accurately replicate in vivo conditions. We present a novel in silico model of 3D mesenchymal cell migration regulated by the chemical and mechanical profile of the surrounding environment. This in silico model considers cell’s adhesive and nuclear phenotypes, the effects of the steric hindrance of the matrix, and cells ability to degradate the ECM. These factors are crucial when investigating the increasing difficulty that migrating cells find to squeeze their nuclei through dense matrices, which may act as physical barriers. Our results agree with previous in vitro observations where fibroblasts cultured in collagen-based hydrogels did not durotax toward regions with higher collagen concentrations. Instead, they exhibited an adurotactic behavior, following a more random trajectory. Overall, cell’s migratory response in 3D domains depends on its phenotype, and the properties of the surrounding environment, that is, 3D cell motion is strongly dependent on the context. |
| first_indexed | 2024-12-11T19:19:28Z |
| format | Article |
| id | doaj.art-ed4f002734b74e71a6a934828ceba232 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-12-11T19:19:28Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-ed4f002734b74e71a6a934828ceba2322022-12-22T00:53:34ZengElsevierEuropean Journal of Cell Biology0171-93352022-06-011013151255A mechanistic protrusive-based model for 3D cell migrationFrancisco Merino-Casallo0Maria Jose Gomez-Benito1Ruben Martinez-Cantin2Jose Manuel Garcia-Aznar3Multiscale in Mechanical and Biological Engineering (M2BE), Aragon Institute of Engineering Research (I3A), Zaragoza 50018, Spain; Department of Mechanical Engineering, Universidad de Zaragoza, Zaragoza 50009, SpainMultiscale in Mechanical and Biological Engineering (M2BE), Aragon Institute of Engineering Research (I3A), Zaragoza 50018, Spain; Department of Mechanical Engineering, Universidad de Zaragoza, Zaragoza 50009, SpainRobotics, Perception and Real Time Group (RoPeRT), Aragon Institute of Engineering Research (I3A), Zaragoza 50018, Spain; Department of Computer Science and System Engineering, Universidad de Zaragoza, Zaragoza 50009, SpainMultiscale in Mechanical and Biological Engineering (M2BE), Aragon Institute of Engineering Research (I3A), Zaragoza 50018, Spain; Department of Mechanical Engineering, Universidad de Zaragoza, Zaragoza 50009, Spain; Corresponding author at: Corresponding author.Cell migration is essential for a variety of biological processes, such as embryogenesis, wound healing, and the immune response. After more than a century of research—mainly on flat surfaces—, there are still many unknowns about cell motility. In particular, regarding how cells migrate within 3D matrices, which more accurately replicate in vivo conditions. We present a novel in silico model of 3D mesenchymal cell migration regulated by the chemical and mechanical profile of the surrounding environment. This in silico model considers cell’s adhesive and nuclear phenotypes, the effects of the steric hindrance of the matrix, and cells ability to degradate the ECM. These factors are crucial when investigating the increasing difficulty that migrating cells find to squeeze their nuclei through dense matrices, which may act as physical barriers. Our results agree with previous in vitro observations where fibroblasts cultured in collagen-based hydrogels did not durotax toward regions with higher collagen concentrations. Instead, they exhibited an adurotactic behavior, following a more random trajectory. Overall, cell’s migratory response in 3D domains depends on its phenotype, and the properties of the surrounding environment, that is, 3D cell motion is strongly dependent on the context.http://www.sciencedirect.com/science/article/pii/S01719335220005893D cell migrationProtrusion dynamicsCell mechanicsCell - matrix interactionsMatrix mechanicsMatrix remodeling |
| spellingShingle | Francisco Merino-Casallo Maria Jose Gomez-Benito Ruben Martinez-Cantin Jose Manuel Garcia-Aznar A mechanistic protrusive-based model for 3D cell migration European Journal of Cell Biology 3D cell migration Protrusion dynamics Cell mechanics Cell - matrix interactions Matrix mechanics Matrix remodeling |
| title | A mechanistic protrusive-based model for 3D cell migration |
| title_full | A mechanistic protrusive-based model for 3D cell migration |
| title_fullStr | A mechanistic protrusive-based model for 3D cell migration |
| title_full_unstemmed | A mechanistic protrusive-based model for 3D cell migration |
| title_short | A mechanistic protrusive-based model for 3D cell migration |
| title_sort | mechanistic protrusive based model for 3d cell migration |
| topic | 3D cell migration Protrusion dynamics Cell mechanics Cell - matrix interactions Matrix mechanics Matrix remodeling |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000589 |
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