Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes
Background/purpose: Progesterone and estrogen levels are elevated during pregnancy and play a role in maternal immune responses. In addition, unbalanced metabolism of growth factors has been demonstrated in pregnancy tumors. Therefore, we aimed to investigate the effect of progesterone and β-estradi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013-12-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790213001359 |
| _version_ | 1818497977000394752 |
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| author | Paiboon Jitprasertwong Niratcha Chaisomboon Kusuma Jamdee |
| author_facet | Paiboon Jitprasertwong Niratcha Chaisomboon Kusuma Jamdee |
| author_sort | Paiboon Jitprasertwong |
| collection | DOAJ |
| description | Background/purpose: Progesterone and estrogen levels are elevated during pregnancy and play a role in maternal immune responses. In addition, unbalanced metabolism of growth factors has been demonstrated in pregnancy tumors. Therefore, we aimed to investigate the effect of progesterone and β-estradiol on vascular endothelial growth factor-A (VEGF-A) and basic fibroblast growth factor (bFGF) messenger RNA (mRNA) expression in THP-1 monocytes in response to Porphyromonas gingivalis lipopolysaccharide (LPS).
Materials and methods: THP-1 monocytes were incubated with progesterone, β-estradiol, or LPS from Escherichia coli and P. gingivalis for up to 24 hours. The expression of VEGF-A and bFGF was investigated using conventional reverse transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR. Cell growth was assessed using cell proliferation assay.
Results: We reported herein that progesterone, but not β-estradiol, increased VEGF-A mRNA expression in THP-1 monocytes. Significantly, progesterone enhanced VEGF-A mRNA expression in P. gingivalis LPS-treated monocytes in comparison with a treatment with P. gingivalis LPS alone. However, neither β-estradiol nor progesterone had any effect on bFGF production at mRNA levels.
Conclusion: The enhancing effect of progesterone on VEGF-A mRNA expression may have a role in the pathogenesis of pyogenic granuloma in pregnant women. |
| first_indexed | 2024-12-10T18:52:07Z |
| format | Article |
| id | doaj.art-ed4fd8041e5d421fa87d99c256e7931b |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-10T18:52:07Z |
| publishDate | 2013-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-ed4fd8041e5d421fa87d99c256e7931b2022-12-22T01:37:17ZengElsevierJournal of Dental Sciences1991-79022013-12-018435836410.1016/j.jds.2013.07.001Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytesPaiboon JitprasertwongNiratcha ChaisomboonKusuma JamdeeBackground/purpose: Progesterone and estrogen levels are elevated during pregnancy and play a role in maternal immune responses. In addition, unbalanced metabolism of growth factors has been demonstrated in pregnancy tumors. Therefore, we aimed to investigate the effect of progesterone and β-estradiol on vascular endothelial growth factor-A (VEGF-A) and basic fibroblast growth factor (bFGF) messenger RNA (mRNA) expression in THP-1 monocytes in response to Porphyromonas gingivalis lipopolysaccharide (LPS). Materials and methods: THP-1 monocytes were incubated with progesterone, β-estradiol, or LPS from Escherichia coli and P. gingivalis for up to 24 hours. The expression of VEGF-A and bFGF was investigated using conventional reverse transcription polymerase chain reaction (RT-PCR) and real-time RT-PCR. Cell growth was assessed using cell proliferation assay. Results: We reported herein that progesterone, but not β-estradiol, increased VEGF-A mRNA expression in THP-1 monocytes. Significantly, progesterone enhanced VEGF-A mRNA expression in P. gingivalis LPS-treated monocytes in comparison with a treatment with P. gingivalis LPS alone. However, neither β-estradiol nor progesterone had any effect on bFGF production at mRNA levels. Conclusion: The enhancing effect of progesterone on VEGF-A mRNA expression may have a role in the pathogenesis of pyogenic granuloma in pregnant women.http://www.sciencedirect.com/science/article/pii/S1991790213001359estrogenmonocytesP. gingivalisprogesteroneVEGF-A |
| spellingShingle | Paiboon Jitprasertwong Niratcha Chaisomboon Kusuma Jamdee Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes Journal of Dental Sciences estrogen monocytes P. gingivalis progesterone VEGF-A |
| title | Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes |
| title_full | Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes |
| title_fullStr | Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes |
| title_full_unstemmed | Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes |
| title_short | Progesterone, but not β-estradiol, enhances Porphyromonas gingivalis lipopolysaccharide-induced vascular endothelial growth factor-A expression in human THP-1 monocytes |
| title_sort | progesterone but not β estradiol enhances porphyromonas gingivalis lipopolysaccharide induced vascular endothelial growth factor a expression in human thp 1 monocytes |
| topic | estrogen monocytes P. gingivalis progesterone VEGF-A |
| url | http://www.sciencedirect.com/science/article/pii/S1991790213001359 |
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