In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai
Background: : Pangdahai (matured, ripened, and dried seeds of Scaphium affine (Mast.) Pierre) is widely used in managing several diseases in countries like China, Vietnam, Japan, and India. This study evaluated the anti-inflammatory effects of the crude extracts (ethanol and aqueous) and isolated co...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324000113 |
| _version_ | 1797305264241639424 |
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| author | Mahmood B. Oppong Shijie Cao Shi-Ming Fang Seth K. Amponsah Paul O. Donkor Michael Lartey Lawrence A. Adutwum Kwabena F.M. Opuni Feng Zhao Qiu Feng |
| author_facet | Mahmood B. Oppong Shijie Cao Shi-Ming Fang Seth K. Amponsah Paul O. Donkor Michael Lartey Lawrence A. Adutwum Kwabena F.M. Opuni Feng Zhao Qiu Feng |
| author_sort | Mahmood B. Oppong |
| collection | DOAJ |
| description | Background: : Pangdahai (matured, ripened, and dried seeds of Scaphium affine (Mast.) Pierre) is widely used in managing several diseases in countries like China, Vietnam, Japan, and India. This study evaluated the anti-inflammatory effects of the crude extracts (ethanol and aqueous) and isolated compounds of Pangdahai. Methods: : Xylene-induced ear edema in mice, carrageenan-induced paw edema in rats, and nitric oxide (NO) assay were used to evaluate and screen the crude extracts and isolated compounds from the ethanolic extracts of Pangdahai. TNF-α and IL-1β levels in the tissues of rat foot and ear were determined by ELISA. The cytotoxicity of the isolated compounds was also determined by MTT assay. Molecular docking studies using targets involved in the inflammatory process were also used to further evaluate the compounds. Results: : Both aqueous and ethanol extracts demonstrated significant anti-inflammatory effect and markedly attenuated vascular permeability in mice induced by acetic acid in a dose-independent manner. The ethanol extract also significantly inhibited levels of IL-1β and TNF-α. Four (4) compounds exhibited significant inhibitory effects on NO release without cytotoxicity on RAW 264.7 macrophage. These compounds also showed good binding affinities for COX-2, PLA2, IRAK-4 and NIK. Conclusions: This study validates, provides scientific evidence and justification for the use of the aqueous decoctions of Pangdahai in pharyngitis traditionally. (+) – Pinoresinol, tiliroside, Z-caffeic acid, and 3,4-dihydroxybenzoic acid (protocatechuic acid) isolated from Pangdahai showed anti-inflammatory activities, which might be responsible for the actions of Pangdahai. Tiliroside showed high binding affinity comparable to the native ligands of inflammatory mediators. |
| first_indexed | 2024-03-08T00:23:24Z |
| format | Article |
| id | doaj.art-ed57238557634dca8154956950fcf4c8 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-03-08T00:23:24Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-ed57238557634dca8154956950fcf4c82024-02-16T04:30:32ZengElsevierPhytomedicine Plus2667-03132024-05-0142100533In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of PangdahaiMahmood B. Oppong0Shijie Cao1Shi-Ming Fang2Seth K. Amponsah3Paul O. Donkor4Michael Lartey5Lawrence A. Adutwum6Kwabena F.M. Opuni7Feng Zhao8Qiu Feng9Department of Pharmaceutical Chemistry, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, Ghana; Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin, 301617, China; Corresponding author at: Department of Pharmaceutical Chemistry, School of Pharmacy, College of Health Sciences, University of Ghana, P.O. Box LG 43, Legon, Ghana.Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin, 301617, ChinaTianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin, 301617, ChinaDepartment of Medical Pharmacology, University of Ghana Medical School, Accra, GhanaDepartment of Pharmacognosy and Herbal Medicine, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, GhanaDepartment of Pharmaceutical Chemistry, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, GhanaDepartment of Pharmaceutical Chemistry, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, GhanaDepartment of Pharmaceutical Chemistry, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, GhanaSchool of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai, 264005, People's Republic of ChinaTianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin, 301617, ChinaBackground: : Pangdahai (matured, ripened, and dried seeds of Scaphium affine (Mast.) Pierre) is widely used in managing several diseases in countries like China, Vietnam, Japan, and India. This study evaluated the anti-inflammatory effects of the crude extracts (ethanol and aqueous) and isolated compounds of Pangdahai. Methods: : Xylene-induced ear edema in mice, carrageenan-induced paw edema in rats, and nitric oxide (NO) assay were used to evaluate and screen the crude extracts and isolated compounds from the ethanolic extracts of Pangdahai. TNF-α and IL-1β levels in the tissues of rat foot and ear were determined by ELISA. The cytotoxicity of the isolated compounds was also determined by MTT assay. Molecular docking studies using targets involved in the inflammatory process were also used to further evaluate the compounds. Results: : Both aqueous and ethanol extracts demonstrated significant anti-inflammatory effect and markedly attenuated vascular permeability in mice induced by acetic acid in a dose-independent manner. The ethanol extract also significantly inhibited levels of IL-1β and TNF-α. Four (4) compounds exhibited significant inhibitory effects on NO release without cytotoxicity on RAW 264.7 macrophage. These compounds also showed good binding affinities for COX-2, PLA2, IRAK-4 and NIK. Conclusions: This study validates, provides scientific evidence and justification for the use of the aqueous decoctions of Pangdahai in pharyngitis traditionally. (+) – Pinoresinol, tiliroside, Z-caffeic acid, and 3,4-dihydroxybenzoic acid (protocatechuic acid) isolated from Pangdahai showed anti-inflammatory activities, which might be responsible for the actions of Pangdahai. Tiliroside showed high binding affinity comparable to the native ligands of inflammatory mediators.http://www.sciencedirect.com/science/article/pii/S2667031324000113PangdahaiAnti-inflammationCytotoxicitySecondary metabolites |
| spellingShingle | Mahmood B. Oppong Shijie Cao Shi-Ming Fang Seth K. Amponsah Paul O. Donkor Michael Lartey Lawrence A. Adutwum Kwabena F.M. Opuni Feng Zhao Qiu Feng In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai Phytomedicine Plus Pangdahai Anti-inflammation Cytotoxicity Secondary metabolites |
| title | In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai |
| title_full | In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai |
| title_fullStr | In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai |
| title_full_unstemmed | In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai |
| title_short | In-vitro and in-vivo anti-inflammatory properties of extracts and isolates of Pangdahai |
| title_sort | in vitro and in vivo anti inflammatory properties of extracts and isolates of pangdahai |
| topic | Pangdahai Anti-inflammation Cytotoxicity Secondary metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324000113 |
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