Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma
Background: Increasing evidence has shown that tumor initiation and growth are nourished by a small subpopulation of cancer stem cells (CSCs) within the tumor mass. CSCs are posited to be responsible for tumor maintenance, growth, distant metastasis, and relapse after curative operation. We examined...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Pathologists & the Korean Society for Cytopathology
2016-01-01
|
| Series: | Journal of Pathology and Translational Medicine |
| Subjects: | |
| Online Access: | http://www.jpatholtm.org/upload/pdf/jptm-2015-10-09.pdf |
| _version_ | 1818965024827244544 |
|---|---|
| author | Jong Jin Sung Sang Jae Noh Jun Sang Bae Ho Sung Park Kyu Yun Jang Myoung Ja Chung Woo Sung Moon |
| author_facet | Jong Jin Sung Sang Jae Noh Jun Sang Bae Ho Sung Park Kyu Yun Jang Myoung Ja Chung Woo Sung Moon |
| author_sort | Jong Jin Sung |
| collection | DOAJ |
| description | Background: Increasing evidence has shown that tumor initiation and growth are nourished by a small subpopulation of cancer stem cells (CSCs) within the tumor mass. CSCs are posited to be responsible for tumor maintenance, growth, distant metastasis, and relapse after curative operation. We examined the expression of CSC markers in paraffin-embedded tissue sections of hepatocellular carcinoma (HCC) and correlated the results with clinicopathologic characteristics. Methods: Immunohistochemical staining for the markers believed to be expressed in the CSCs, including epithelial cell adhesion molecule (EpCAM), keratin 19 (K19), CD133, and CD56, was performed in 82 HCC specimens. Results: EpCAM expression was observed in 56% of the HCCs (46/82) and K19 in 6% (5/82). EpCAM expression in HCC significantly correlated with elevated α-fetoprotein level, microvessel invasion of tumor cells, and high histologic grade. In addition, EpCAM expression significantly correlated with K19 expression. The overall survival and relapsefree survival rates in patients with EpCAM-expressing HCC were relatively lower than those in patients with EpCAM-negative HCC. All but two of the 82 HCCs were negative for CD133 and CD56, respectively. Conclusions: Our results suggest that HCCs expressing EpCAM are associated with unfavorable prognostic factors and have a more aggressive clinical course than those not expressing EpCAM. Further, the expression of either CD133 or CD56 in paraffin-embedded HCC tissues appears to be rare. |
| first_indexed | 2024-12-20T13:10:26Z |
| format | Article |
| id | doaj.art-ed5efcaef2ab44c494dc09befe0ad1e9 |
| institution | Directory Open Access Journal |
| issn | 2383-7837 2383-7845 |
| language | English |
| last_indexed | 2024-12-20T13:10:26Z |
| publishDate | 2016-01-01 |
| publisher | Korean Society of Pathologists & the Korean Society for Cytopathology |
| record_format | Article |
| series | Journal of Pathology and Translational Medicine |
| spelling | doaj.art-ed5efcaef2ab44c494dc09befe0ad1e92022-12-21T19:39:41ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452016-01-01501525710.4132/jptm.2015.10.0916575Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular CarcinomaJong Jin SungSang Jae NohJun Sang BaeHo Sung ParkKyu Yun JangMyoung Ja ChungWoo Sung MoonBackground: Increasing evidence has shown that tumor initiation and growth are nourished by a small subpopulation of cancer stem cells (CSCs) within the tumor mass. CSCs are posited to be responsible for tumor maintenance, growth, distant metastasis, and relapse after curative operation. We examined the expression of CSC markers in paraffin-embedded tissue sections of hepatocellular carcinoma (HCC) and correlated the results with clinicopathologic characteristics. Methods: Immunohistochemical staining for the markers believed to be expressed in the CSCs, including epithelial cell adhesion molecule (EpCAM), keratin 19 (K19), CD133, and CD56, was performed in 82 HCC specimens. Results: EpCAM expression was observed in 56% of the HCCs (46/82) and K19 in 6% (5/82). EpCAM expression in HCC significantly correlated with elevated α-fetoprotein level, microvessel invasion of tumor cells, and high histologic grade. In addition, EpCAM expression significantly correlated with K19 expression. The overall survival and relapsefree survival rates in patients with EpCAM-expressing HCC were relatively lower than those in patients with EpCAM-negative HCC. All but two of the 82 HCCs were negative for CD133 and CD56, respectively. Conclusions: Our results suggest that HCCs expressing EpCAM are associated with unfavorable prognostic factors and have a more aggressive clinical course than those not expressing EpCAM. Further, the expression of either CD133 or CD56 in paraffin-embedded HCC tissues appears to be rare.http://www.jpatholtm.org/upload/pdf/jptm-2015-10-09.pdfCarcinoma, hepatocellularEpCAM proteinCancer stem cells |
| spellingShingle | Jong Jin Sung Sang Jae Noh Jun Sang Bae Ho Sung Park Kyu Yun Jang Myoung Ja Chung Woo Sung Moon Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma Journal of Pathology and Translational Medicine Carcinoma, hepatocellular EpCAM protein Cancer stem cells |
| title | Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma |
| title_full | Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma |
| title_fullStr | Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma |
| title_full_unstemmed | Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma |
| title_short | Immunohistochemical Expression and Clinical Significance of Suggested Stem Cell Markers in Hepatocellular Carcinoma |
| title_sort | immunohistochemical expression and clinical significance of suggested stem cell markers in hepatocellular carcinoma |
| topic | Carcinoma, hepatocellular EpCAM protein Cancer stem cells |
| url | http://www.jpatholtm.org/upload/pdf/jptm-2015-10-09.pdf |
| work_keys_str_mv | AT jongjinsung immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT sangjaenoh immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT junsangbae immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT hosungpark immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT kyuyunjang immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT myoungjachung immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma AT woosungmoon immunohistochemicalexpressionandclinicalsignificanceofsuggestedstemcellmarkersinhepatocellularcarcinoma |