Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures.
Surveillance of bloodstream infections (BSI) is a high priority within the hospital setting. Broth-based blood cultures are the current gold standard for detecting BSI, however they can require lengthy incubation periods prior to detection of positive samples. We set out to demonstrate the feasibili...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3796490?pdf=render |
| _version_ | 1811200958060822528 |
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| author | Daniel R Zweitzig Nichol M Riccardello John Morrison Jason Rubino Jennifer Axelband Rebecca Jeanmonod Bruce I Sodowich Mark J Kopnitsky S Mark O'Hara |
| author_facet | Daniel R Zweitzig Nichol M Riccardello John Morrison Jason Rubino Jennifer Axelband Rebecca Jeanmonod Bruce I Sodowich Mark J Kopnitsky S Mark O'Hara |
| author_sort | Daniel R Zweitzig |
| collection | DOAJ |
| description | Surveillance of bloodstream infections (BSI) is a high priority within the hospital setting. Broth-based blood cultures are the current gold standard for detecting BSI, however they can require lengthy incubation periods prior to detection of positive samples. We set out to demonstrate the feasibility of using enzymatic template generation and amplification (ETGA)-mediated measurement of DNA polymerase activity to detect microbes from clinical blood cultures. In addition to routine-collected hospital blood cultures, one parallel aerobic blood culture was collected and immediately refrigerated until being transported for ETGA analysis. After refrigeration holding and transport, parallel-collected cultures were placed into a BACTEC incubator and ETGA time-course analysis was performed. Of the 308 clinical blood cultures received, 22 were BACTEC positive, and thus were initially selected for ETGA time course analysis. The ETGA assay detected microbial growth in all 22 parallel-positive blood cultures in less time than a BACTEC incubator and also yielded genomic DNA for qPCR-based organism identification. In summary, feasibility of detecting microbes from clinical blood culture samples using the ETGA blood culture assay was demonstrated. Additional studies are being considered towards development of clinically beneficial versions of this methodology. |
| first_indexed | 2024-04-12T02:12:58Z |
| format | Article |
| id | doaj.art-ed609ebdea3c43c183f294e70f08c9b5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T02:12:58Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ed609ebdea3c43c183f294e70f08c9b52022-12-22T03:52:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7848810.1371/journal.pone.0078488Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures.Daniel R ZweitzigNichol M RiccardelloJohn MorrisonJason RubinoJennifer AxelbandRebecca JeanmonodBruce I SodowichMark J KopnitskyS Mark O'HaraSurveillance of bloodstream infections (BSI) is a high priority within the hospital setting. Broth-based blood cultures are the current gold standard for detecting BSI, however they can require lengthy incubation periods prior to detection of positive samples. We set out to demonstrate the feasibility of using enzymatic template generation and amplification (ETGA)-mediated measurement of DNA polymerase activity to detect microbes from clinical blood cultures. In addition to routine-collected hospital blood cultures, one parallel aerobic blood culture was collected and immediately refrigerated until being transported for ETGA analysis. After refrigeration holding and transport, parallel-collected cultures were placed into a BACTEC incubator and ETGA time-course analysis was performed. Of the 308 clinical blood cultures received, 22 were BACTEC positive, and thus were initially selected for ETGA time course analysis. The ETGA assay detected microbial growth in all 22 parallel-positive blood cultures in less time than a BACTEC incubator and also yielded genomic DNA for qPCR-based organism identification. In summary, feasibility of detecting microbes from clinical blood culture samples using the ETGA blood culture assay was demonstrated. Additional studies are being considered towards development of clinically beneficial versions of this methodology.http://europepmc.org/articles/PMC3796490?pdf=render |
| spellingShingle | Daniel R Zweitzig Nichol M Riccardello John Morrison Jason Rubino Jennifer Axelband Rebecca Jeanmonod Bruce I Sodowich Mark J Kopnitsky S Mark O'Hara Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. PLoS ONE |
| title | Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. |
| title_full | Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. |
| title_fullStr | Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. |
| title_full_unstemmed | Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. |
| title_short | Measurement of microbial DNA polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures. |
| title_sort | measurement of microbial dna polymerase activity enables detection and growth monitoring of microbes from clinical blood cultures |
| url | http://europepmc.org/articles/PMC3796490?pdf=render |
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