Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow
Pluripotent stem cells (PSCs) are considered as being an important cell source for regenerative medicine. The culture of PSCs usually requires a feeder cell layer or cell adhesive matrix coating such as Matrigel, laminin, and gelatin. Although a feeder-free culture using a matrix coating has been po...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/8/734 |
| _version_ | 1827712187227963392 |
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| author | Yuuwa Takahashi Shogo Miyata |
| author_facet | Yuuwa Takahashi Shogo Miyata |
| author_sort | Yuuwa Takahashi |
| collection | DOAJ |
| description | Pluripotent stem cells (PSCs) are considered as being an important cell source for regenerative medicine. The culture of PSCs usually requires a feeder cell layer or cell adhesive matrix coating such as Matrigel, laminin, and gelatin. Although a feeder-free culture using a matrix coating has been popular, the on-feeder culture is still an effective method for the fundamental study of regenerative medicine and stem cell biology. To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-effective cell sorting technology is required. There are several commercialized cell-sorting methods, such as FACS or MACS. However, these methods require cell labeling by fluorescent dye or magnetic antibodies with complicated processes. To resolve these problems, we focused on dielectrophoresis (DEP) phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies. DEP imposes an electric force on living cells under a non-uniform AC electric field. The direction and magnitude of the DEP force depend on the electric property and size of the cell. Therefore, DEP is considered as a promising approach for sorting PSCs from feeder cells. In this study, we developed a simple continuous cell-sorting device using the DEP force and fluid-induced shear force. As a result, mouse embryonic stem cells (mESCs) were purified from a mixed-cell suspension containing mESCs and mouse embryonic fibroblasts (MEFs) using our DEP cell-sorting device. |
| first_indexed | 2024-03-10T18:08:39Z |
| format | Article |
| id | doaj.art-ed62d46525fc403a89acc0028565c37e |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T18:08:39Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-ed62d46525fc403a89acc0028565c37e2023-11-20T08:19:58ZengMDPI AGMicromachines2072-666X2020-07-0111873410.3390/mi11080734Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid FlowYuuwa Takahashi0Shogo Miyata1Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, JapanDepartment of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, JapanPluripotent stem cells (PSCs) are considered as being an important cell source for regenerative medicine. The culture of PSCs usually requires a feeder cell layer or cell adhesive matrix coating such as Matrigel, laminin, and gelatin. Although a feeder-free culture using a matrix coating has been popular, the on-feeder culture is still an effective method for the fundamental study of regenerative medicine and stem cell biology. To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-effective cell sorting technology is required. There are several commercialized cell-sorting methods, such as FACS or MACS. However, these methods require cell labeling by fluorescent dye or magnetic antibodies with complicated processes. To resolve these problems, we focused on dielectrophoresis (DEP) phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies. DEP imposes an electric force on living cells under a non-uniform AC electric field. The direction and magnitude of the DEP force depend on the electric property and size of the cell. Therefore, DEP is considered as a promising approach for sorting PSCs from feeder cells. In this study, we developed a simple continuous cell-sorting device using the DEP force and fluid-induced shear force. As a result, mouse embryonic stem cells (mESCs) were purified from a mixed-cell suspension containing mESCs and mouse embryonic fibroblasts (MEFs) using our DEP cell-sorting device.https://www.mdpi.com/2072-666X/11/8/734cell sortingdielectrophoresisfluid-induced shear forcepluripotent stem cellfeeder cell |
| spellingShingle | Yuuwa Takahashi Shogo Miyata Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow Micromachines cell sorting dielectrophoresis fluid-induced shear force pluripotent stem cell feeder cell |
| title | Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow |
| title_full | Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow |
| title_fullStr | Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow |
| title_full_unstemmed | Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow |
| title_short | Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow |
| title_sort | continuous es feeder cell sorting device using dielectrophoresis and controlled fluid flow |
| topic | cell sorting dielectrophoresis fluid-induced shear force pluripotent stem cell feeder cell |
| url | https://www.mdpi.com/2072-666X/11/8/734 |
| work_keys_str_mv | AT yuuwatakahashi continuousesfeedercellsortingdeviceusingdielectrophoresisandcontrolledfluidflow AT shogomiyata continuousesfeedercellsortingdeviceusingdielectrophoresisandcontrolledfluidflow |