The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics
Plastic pollution in marine ecosystems is one of the most important study topics in recent years. The toxicity, mobility and long-term persistence characteristics of plastics create risk in ecosystems, biota and human health. In this study, the levels of heterotrophic bacteria attached to the surfac...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2020-03-01
|
| Series: | Aquatic Sciences and Engineering |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ase/issue/52069/679538?publisher=istanbul |
| _version_ | 1797226389506621440 |
|---|---|
| author | Pelin Saliha ÇİFTÇİ TÜRETKEN Gülşen ALTUĞ Turgay ÖKSÜZOĞLU |
| author_facet | Pelin Saliha ÇİFTÇİ TÜRETKEN Gülşen ALTUĞ Turgay ÖKSÜZOĞLU |
| author_sort | Pelin Saliha ÇİFTÇİ TÜRETKEN |
| collection | DOAJ |
| description | Plastic pollution in marine ecosystems is one of the most important study topics in recent years. The toxicity, mobility and long-term persistence characteristics of plastics create risk in ecosystems, biota and human health. In this study, the levels of heterotrophic bacteria attached to the surfaces of commonly used plastic types; polyvinylchloride (PVC), polyethylene (PE), and polypropylene (PP) were tested in a mechanical experimental system prepared with seawater under controlled conditions in laboratory. The seawater, which was used in the experimental system, was taken under aseptic conditions from the Golden Horn Estuary, located in the Istanbul region of Turkey. Three different types of plastic (PVC, PE and PP), in two different (glass slide (76x26 cm) and virgin micro pellets (5mm diameter) size, were placed in the experiment setup filled with seawater and incubated for 28 days at ambient temperature. At the end of 28 days, the counts of heterotrophic bacteria were tested using the spread plate technique on Marine Agar (Difco), in both plastic surfaces and surrounding seawater. The levels of heterotrophic bacteria were recorded to be lower in the seawater surrounding the micropellets and lam-size plastic samples. The seawater sample bacterial levels were recorded as 12x109 CFU/ml, at the start of the experiment. At the end of the 28th days, it was recorded to be 83x109 CFU/ml. The highest levels of heterotrophic bacteria were recorded as 41x1010 CFU /cm-2 and 61x1010 CFU /cm-2 on the lam-size surfaces and the micropellet surface of the polypropylene samples, respectively. In the experiments, the PP plastic type has been recorded as a more preferred plastic derivative by heterotrophic bacteria according to the PVC and PE plastic types, but there has been no significant difference in the bacterial adhesion rates on the surfaces. The study contributed increasing knowledge on the bacterial approach to microplastics types. However, there is a need for long term studies related to the mechanism of bacteria attached to microplastics. |
| first_indexed | 2024-04-24T14:24:08Z |
| format | Article |
| id | doaj.art-ed6478ab73e34dcaa746c53f45f1212c |
| institution | Directory Open Access Journal |
| issn | 2602-473X |
| language | English |
| last_indexed | 2024-04-24T14:24:08Z |
| publishDate | 2020-03-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Aquatic Sciences and Engineering |
| spelling | doaj.art-ed6478ab73e34dcaa746c53f45f1212c2024-04-03T05:42:27ZengIstanbul University PressAquatic Sciences and Engineering2602-473X2020-03-01352313510.26650/ASE20206795384The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of PlasticsPelin Saliha ÇİFTÇİ TÜRETKENGülşen ALTUĞTurgay ÖKSÜZOĞLUPlastic pollution in marine ecosystems is one of the most important study topics in recent years. The toxicity, mobility and long-term persistence characteristics of plastics create risk in ecosystems, biota and human health. In this study, the levels of heterotrophic bacteria attached to the surfaces of commonly used plastic types; polyvinylchloride (PVC), polyethylene (PE), and polypropylene (PP) were tested in a mechanical experimental system prepared with seawater under controlled conditions in laboratory. The seawater, which was used in the experimental system, was taken under aseptic conditions from the Golden Horn Estuary, located in the Istanbul region of Turkey. Three different types of plastic (PVC, PE and PP), in two different (glass slide (76x26 cm) and virgin micro pellets (5mm diameter) size, were placed in the experiment setup filled with seawater and incubated for 28 days at ambient temperature. At the end of 28 days, the counts of heterotrophic bacteria were tested using the spread plate technique on Marine Agar (Difco), in both plastic surfaces and surrounding seawater. The levels of heterotrophic bacteria were recorded to be lower in the seawater surrounding the micropellets and lam-size plastic samples. The seawater sample bacterial levels were recorded as 12x109 CFU/ml, at the start of the experiment. At the end of the 28th days, it was recorded to be 83x109 CFU/ml. The highest levels of heterotrophic bacteria were recorded as 41x1010 CFU /cm-2 and 61x1010 CFU /cm-2 on the lam-size surfaces and the micropellet surface of the polypropylene samples, respectively. In the experiments, the PP plastic type has been recorded as a more preferred plastic derivative by heterotrophic bacteria according to the PVC and PE plastic types, but there has been no significant difference in the bacterial adhesion rates on the surfaces. The study contributed increasing knowledge on the bacterial approach to microplastics types. However, there is a need for long term studies related to the mechanism of bacteria attached to microplastics.https://dergipark.org.tr/tr/pub/ase/issue/52069/679538?publisher=istanbulmicroplasticheterotrophic bacteriapolyvinylchloridepolyethylenepolypropylene |
| spellingShingle | Pelin Saliha ÇİFTÇİ TÜRETKEN Gülşen ALTUĞ Turgay ÖKSÜZOĞLU The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics Aquatic Sciences and Engineering microplastic heterotrophic bacteria polyvinylchloride polyethylene polypropylene |
| title | The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics |
| title_full | The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics |
| title_fullStr | The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics |
| title_full_unstemmed | The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics |
| title_short | The Levels of Plastic-associated Heterotrophic Bacteria on Three Different Types of Plastics |
| title_sort | levels of plastic associated heterotrophic bacteria on three different types of plastics |
| topic | microplastic heterotrophic bacteria polyvinylchloride polyethylene polypropylene |
| url | https://dergipark.org.tr/tr/pub/ase/issue/52069/679538?publisher=istanbul |
| work_keys_str_mv | AT pelinsalihaciftcituretken thelevelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics AT gulsenaltug thelevelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics AT turgayoksuzoglu thelevelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics AT pelinsalihaciftcituretken levelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics AT gulsenaltug levelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics AT turgayoksuzoglu levelsofplasticassociatedheterotrophicbacteriaonthreedifferenttypesofplastics |