Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+

Gas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.

Bibliographic Details
Main Authors: V. Atanasov, V. Bardarov, B. Aleksiev, M. Mitewa
Format: Article
Language:English
Published: SciELO 2005-09-01
Series:Journal of Venomous Animals and Toxins including Tropical Diseases
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300011
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author V. Atanasov
V. Bardarov
B. Aleksiev
M. Mitewa
author_facet V. Atanasov
V. Bardarov
B. Aleksiev
M. Mitewa
author_sort V. Atanasov
collection DOAJ
description Gas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.
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spelling doaj.art-ed6c2732301c488dbe1f7530bd5f0be52022-12-21T18:56:54ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992005-09-0111335036010.1590/S1678-91992005000300011Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+V. AtanasovV. BardarovB. AleksievM. MitewaGas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300011vipoxinphospholipase A2, enzyme specificityCa2+Mg2+Sr2+ influence
spellingShingle V. Atanasov
V. Bardarov
B. Aleksiev
M. Mitewa
Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
Journal of Venomous Animals and Toxins including Tropical Diseases
vipoxin
phospholipase A2, enzyme specificity
Ca2+
Mg2+
Sr2+ influence
title Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
title_full Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
title_fullStr Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
title_full_unstemmed Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
title_short Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+
title_sort vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products influence of ca2 mg2 and sr2
topic vipoxin
phospholipase A2, enzyme specificity
Ca2+
Mg2+
Sr2+ influence
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300011
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AT vbardarov vipoxinspecificitystudiedbygaschromatographicdeterminationofenzymaticreactionproductsinfluenceofca2mg2andsr2
AT baleksiev vipoxinspecificitystudiedbygaschromatographicdeterminationofenzymaticreactionproductsinfluenceofca2mg2andsr2
AT mmitewa vipoxinspecificitystudiedbygaschromatographicdeterminationofenzymaticreactionproductsinfluenceofca2mg2andsr2