ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells
Abstract Colorectal cancer (CRC) is a worldwide health concern. Chronic inflammation is a risk factor for CRC, and interleukin-6 (IL-6) plays a pivotal role in this process. Arginine-specific mono-ADP-ribosyltransferase-1 (ART1) positively regulates inflammatory cytokines. ART1 knockdown reduces the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-024-12120-0 |
| _version_ | 1797247235433431040 |
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| author | Ting Lin Shuxian Zhang Yi Tang Ming Xiao Ming Li Hanjuan Gong Hailun Xie Yalan Wang |
| author_facet | Ting Lin Shuxian Zhang Yi Tang Ming Xiao Ming Li Hanjuan Gong Hailun Xie Yalan Wang |
| author_sort | Ting Lin |
| collection | DOAJ |
| description | Abstract Colorectal cancer (CRC) is a worldwide health concern. Chronic inflammation is a risk factor for CRC, and interleukin-6 (IL-6) plays a pivotal role in this process. Arginine-specific mono-ADP-ribosyltransferase-1 (ART1) positively regulates inflammatory cytokines. ART1 knockdown reduces the level of glycoprotein 130 (gp130), a key transducer in the IL-6 signalling pathway. However, the relationship between ART1 and IL-6 and the resulting effects on IL-6-induced proliferation in CRC cells remain unclear. The aims of this study were to investigate the effects of ART1 knockdown on IL-6-induced cell proliferation in vitro and use an in vivo murine model to observe the growth of transplanted tumours. The results showed that compared with the control, ART1-sh cancer cells induced by IL-6 exhibited reduced viability, a lower rate of colony formation, less DNA synthesis, decreased protein levels of gp130, c-Myc, cyclin D1, Bcl-xL, and a reduced p-STAT3/STAT3 ratio (P < 0.05). Moreover, mice transplanted with ART1-sh CT26 cells that had high levels of IL-6 displayed tumours with smaller volumes (P < 0.05). ART1 and gp130 were colocalized in CT26, LoVo and HCT116 cells, and their expression was positively correlated in human CRC tissues. Overall, ART1 may serve as a promising regulatory factor for IL-6 signalling and a potential therapeutic target for human CRC. |
| first_indexed | 2024-04-24T19:55:28Z |
| format | Article |
| id | doaj.art-ed6f28b475d54a0c983b517e9c18c50a |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-24T19:55:28Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-ed6f28b475d54a0c983b517e9c18c50a2024-03-24T12:23:31ZengBMCBMC Cancer1471-24072024-03-0124111310.1186/s12885-024-12120-0ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cellsTing Lin0Shuxian Zhang1Yi Tang2Ming Xiao3Ming Li4Hanjuan Gong5Hailun Xie6Yalan Wang7Department of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityDepartment of Pathology, Molecular Medicine and Cancer Research Center, Basic Medicine College, Chongqing Medical UniversityAbstract Colorectal cancer (CRC) is a worldwide health concern. Chronic inflammation is a risk factor for CRC, and interleukin-6 (IL-6) plays a pivotal role in this process. Arginine-specific mono-ADP-ribosyltransferase-1 (ART1) positively regulates inflammatory cytokines. ART1 knockdown reduces the level of glycoprotein 130 (gp130), a key transducer in the IL-6 signalling pathway. However, the relationship between ART1 and IL-6 and the resulting effects on IL-6-induced proliferation in CRC cells remain unclear. The aims of this study were to investigate the effects of ART1 knockdown on IL-6-induced cell proliferation in vitro and use an in vivo murine model to observe the growth of transplanted tumours. The results showed that compared with the control, ART1-sh cancer cells induced by IL-6 exhibited reduced viability, a lower rate of colony formation, less DNA synthesis, decreased protein levels of gp130, c-Myc, cyclin D1, Bcl-xL, and a reduced p-STAT3/STAT3 ratio (P < 0.05). Moreover, mice transplanted with ART1-sh CT26 cells that had high levels of IL-6 displayed tumours with smaller volumes (P < 0.05). ART1 and gp130 were colocalized in CT26, LoVo and HCT116 cells, and their expression was positively correlated in human CRC tissues. Overall, ART1 may serve as a promising regulatory factor for IL-6 signalling and a potential therapeutic target for human CRC.https://doi.org/10.1186/s12885-024-12120-0ART1Cell proliferationColorectal cancergp130IL-6 |
| spellingShingle | Ting Lin Shuxian Zhang Yi Tang Ming Xiao Ming Li Hanjuan Gong Hailun Xie Yalan Wang ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells BMC Cancer ART1 Cell proliferation Colorectal cancer gp130 IL-6 |
| title | ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells |
| title_full | ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells |
| title_fullStr | ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells |
| title_full_unstemmed | ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells |
| title_short | ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells |
| title_sort | art1 knockdown decreases the il 6 induced proliferation of colorectal cancer cells |
| topic | ART1 Cell proliferation Colorectal cancer gp130 IL-6 |
| url | https://doi.org/10.1186/s12885-024-12120-0 |
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