A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells

A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells

CRISPR/Cas9 nucleases hold great potential for gene therapy, but they frequently induce unwanted off-target cleavage. We previously developed a GFP activation assay for detection of DNA cleavage in cells. Here, we demonstrate two novel applications of this assay. First, we use this assay to confirm...

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Bibliographic Details
Main Authors: Ziying Hu, Chengdong Zhang, Daqi Wang, Siqi Gao, Sang-Ging Ong, Yongming Wang, Wei V. Zheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
CRISPR
genome editing
DNA cleavage
off-target
PAM
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2021.771248/full

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