Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i>
TGF-β receptors play important roles in mediating TGF-β signals during gonadal development. To identify the functions of TGF-β receptors, including the type I receptor (activin receptor-like kinase 5, <i>ALK5</i>) and type II receptor (bone morphogenetic protein receptor 2, BMPR2), durin...
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MDPI AG
2021-05-01
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| author | Taiming Yan Songpei Zhang Yueping Cai Zhijun Ma Jiayang He Qian Zhang Faqiang Deng Lijuan Ye Hongjun Chen Liang He Jie Luo Deying Yang Zhi He |
| author_facet | Taiming Yan Songpei Zhang Yueping Cai Zhijun Ma Jiayang He Qian Zhang Faqiang Deng Lijuan Ye Hongjun Chen Liang He Jie Luo Deying Yang Zhi He |
| author_sort | Taiming Yan |
| collection | DOAJ |
| description | TGF-β receptors play important roles in mediating TGF-β signals during gonadal development. To identify the functions of TGF-β receptors, including the type I receptor (activin receptor-like kinase 5, <i>ALK5</i>) and type II receptor (bone morphogenetic protein receptor 2, BMPR2), during the gonadal development of <i>S. prenanti</i>, the full-length cDNA sequences of <i>ALK5</i> and <i>BMPR2</i> were isolated and characterized. Their expression patterns in developing gonads and in the gonads of exogenous estradiol (E<sub>2</sub>) -fed fish were analyzed. The cDNAs of <i>ALK5</i> and <i>BMPR2</i> were 1925 bp and 3704 bp in length and encoded 501 and 1070 amino acid residues, respectively. <i>ALK5</i> and <i>BMPR2</i> were mostly expressed in gonads, particularly in cortical alveoli stage ovaries and mid-spermatogenic stage testes; however, the overall level of <i>BMPR2</i> mRNA was higher than that of <i>ALK5</i> during gonadal development. Furthermore, immunohistochemical signals of ALK5 and BMPR2 were mostly detected at chromatin nucleolar oocytes and perinuclear oocytes in ovaries and at spermatocytes and spermatogonia in testes. Exogenous E<sub>2</sub> induces the gonadal expression of <i>ALK5</i> and <i>BMPR2</i>, and <i>BMPR2</i> is more responsive to E<sub>2</sub> than <i>ALK5</i>. These results suggest that ALK5 and BMPR2 might play a potentially vital role in both folliculogenesis and spermatogenesis in <i>S. prenanti</i>. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:32:14Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ed73692b3ff249228cf9d7dc4288251d2023-11-21T19:12:53ZengMDPI AGAnimals2076-26152021-05-01115136510.3390/ani11051365Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i>Taiming Yan0Songpei Zhang1Yueping Cai2Zhijun Ma3Jiayang He4Qian Zhang5Faqiang Deng6Lijuan Ye7Hongjun Chen8Liang He9Jie Luo10Deying Yang11Zhi He12College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaTGF-β receptors play important roles in mediating TGF-β signals during gonadal development. To identify the functions of TGF-β receptors, including the type I receptor (activin receptor-like kinase 5, <i>ALK5</i>) and type II receptor (bone morphogenetic protein receptor 2, BMPR2), during the gonadal development of <i>S. prenanti</i>, the full-length cDNA sequences of <i>ALK5</i> and <i>BMPR2</i> were isolated and characterized. Their expression patterns in developing gonads and in the gonads of exogenous estradiol (E<sub>2</sub>) -fed fish were analyzed. The cDNAs of <i>ALK5</i> and <i>BMPR2</i> were 1925 bp and 3704 bp in length and encoded 501 and 1070 amino acid residues, respectively. <i>ALK5</i> and <i>BMPR2</i> were mostly expressed in gonads, particularly in cortical alveoli stage ovaries and mid-spermatogenic stage testes; however, the overall level of <i>BMPR2</i> mRNA was higher than that of <i>ALK5</i> during gonadal development. Furthermore, immunohistochemical signals of ALK5 and BMPR2 were mostly detected at chromatin nucleolar oocytes and perinuclear oocytes in ovaries and at spermatocytes and spermatogonia in testes. Exogenous E<sub>2</sub> induces the gonadal expression of <i>ALK5</i> and <i>BMPR2</i>, and <i>BMPR2</i> is more responsive to E<sub>2</sub> than <i>ALK5</i>. These results suggest that ALK5 and BMPR2 might play a potentially vital role in both folliculogenesis and spermatogenesis in <i>S. prenanti</i>.https://www.mdpi.com/2076-2615/11/5/1365locationexpression patternimmunoreactivityE<sub>2</sub> |
| spellingShingle | Taiming Yan Songpei Zhang Yueping Cai Zhijun Ma Jiayang He Qian Zhang Faqiang Deng Lijuan Ye Hongjun Chen Liang He Jie Luo Deying Yang Zhi He Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> Animals location expression pattern immunoreactivity E<sub>2</sub> |
| title | Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> |
| title_full | Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> |
| title_fullStr | Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> |
| title_full_unstemmed | Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> |
| title_short | Estradiol Upregulates the Expression of the TGF-β Receptors <i>ALK5</i> and <i>BMPR2</i> during the Gonadal Development of <i>Schizothorax prenanti</i> |
| title_sort | estradiol upregulates the expression of the tgf β receptors i alk5 i and i bmpr2 i during the gonadal development of i schizothorax prenanti i |
| topic | location expression pattern immunoreactivity E<sub>2</sub> |
| url | https://www.mdpi.com/2076-2615/11/5/1365 |
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