Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection
Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/4/674 |
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| author | Fanny Leon Elena Pinchon Nevzat Temurok François Morvan Jean-Jacques Vasseur Martine Clot Vincent Foulongne Jean-François Cantaloube Philippe Vande Perre Jean-Pierre Molès Aurélien Daynès Chantal Fournier-Wirth |
| author_facet | Fanny Leon Elena Pinchon Nevzat Temurok François Morvan Jean-Jacques Vasseur Martine Clot Vincent Foulongne Jean-François Cantaloube Philippe Vande Perre Jean-Pierre Molès Aurélien Daynès Chantal Fournier-Wirth |
| author_sort | Fanny Leon |
| collection | DOAJ |
| description | Arbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections. |
| first_indexed | 2024-03-10T12:56:37Z |
| format | Article |
| id | doaj.art-ed74018ccc3d45e49927525f8e960438 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:56:37Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ed74018ccc3d45e49927525f8e9604382023-11-21T11:53:17ZengMDPI AGMicroorganisms2076-26072021-03-019467410.3390/microorganisms9040674Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus InfectionFanny Leon0Elena Pinchon1Nevzat Temurok2François Morvan3Jean-Jacques Vasseur4Martine Clot5Vincent Foulongne6Jean-François Cantaloube7Philippe Vande Perre8Jean-Pierre Molès9Aurélien Daynès10Chantal Fournier-Wirth11Pathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FranceHORIBA Medical, 34184 Montpellier, FranceInstitut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, FranceInstitut des Biomolecules Max Mousseron (IBMM), Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, FranceHORIBA Medical, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FranceHORIBA Medical, 34184 Montpellier, FrancePathogénèse et Contrôle des Infections Chroniques et Emergentes, Université de Montpellier, Etablissement Français du Sang, Inserm, Université des Antilles, 34184 Montpellier, FranceArbovirus diagnostics on blood from donors and travelers returning from endemic areas is increasingly important for better patient management and epidemiological surveillance. We developed a flexible approach based on a magnetic field-enhanced agglutination (MFEA) readout to detect either genomes or host-derived antibodies. Dengue viruses (DENVs) were selected as models. For genome detection, a pan-flavivirus amplification was performed before capture of biotinylated amplicons between magnetic nanoparticles (MNPs) grafted with DENV probes and anti-biotin antibodies. Magnetization cycles accelerated this chaining process to within 5 min while simple turbidimetry measured the signal. This molecular MFEA readout was evaluated on 43 DENV RNA(+) and 32 DENV RNA(−) samples previously screened by real-time RT-PCR. The sensitivity and the specificity were 88.37% (95% CI, 78.76%–97.95%) and 96.87% (95% CI, 90.84%–100%), respectively. For anti-DENV antibody detection, 103 plasma samples from donors were first screened using ELISA assays. An immunological MFEA readout was then performed by adding MNPs grafted with viral antigens to the samples. Anti-DENV antibodies were detected with a sensitivity and specificity of 90.62% (95% CI, 83.50%–97.76%) and 97.44% (95% CI, 92.48%–100%), respectively. This adaptable approach offers flexibility to platforms dedicated to the screening of emerging infections.https://www.mdpi.com/2076-2607/9/4/674arbovirusinnovative diagnosticmagnetic agglutinationnanoparticlesviral genomesantibodies |
| spellingShingle | Fanny Leon Elena Pinchon Nevzat Temurok François Morvan Jean-Jacques Vasseur Martine Clot Vincent Foulongne Jean-François Cantaloube Philippe Vande Perre Jean-Pierre Molès Aurélien Daynès Chantal Fournier-Wirth Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection Microorganisms arbovirus innovative diagnostic magnetic agglutination nanoparticles viral genomes antibodies |
| title | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
| title_full | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
| title_fullStr | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
| title_full_unstemmed | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
| title_short | Diagnostic Performance of a Magnetic Field-Enhanced Agglutination Readout in Detecting Either Viral Genomes or Host Antibodies in Arbovirus Infection |
| title_sort | diagnostic performance of a magnetic field enhanced agglutination readout in detecting either viral genomes or host antibodies in arbovirus infection |
| topic | arbovirus innovative diagnostic magnetic agglutination nanoparticles viral genomes antibodies |
| url | https://www.mdpi.com/2076-2607/9/4/674 |
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