Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus
Among the different types of microorganisms; the fungal biomass is considered as an effective mediator for bioremediation of heavy metals, due to its higher surface area and extensive hyphal density in the soil. About seven fungal spp. were isolated from the soil garden of the Middle Eastern Regio...
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The National Information and Documentation Centre (NIDOC) , Egypt
2020-10-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_118448_e527a168126e39b893c5f38b8f8d507c.pdf |
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| author | Hosam, El-Sayyad Khaled, F. Elyasergy Tahany, M. Abdel Rahaman Moustafa, A.S. Aly Menna Allah, Ashraf |
| author_facet | Hosam, El-Sayyad Khaled, F. Elyasergy Tahany, M. Abdel Rahaman Moustafa, A.S. Aly Menna Allah, Ashraf |
| author_sort | Hosam, El-Sayyad |
| collection | DOAJ |
| description | Among the different types of microorganisms; the fungal biomass is considered as an effective mediator for
bioremediation of heavy metals, due to its higher surface area and extensive hyphal density in the soil. About
seven fungal spp. were isolated from the soil garden of the Middle Eastern Regional Radioisotope Center for Arab
Countries (MERRCAC); however, a single isolate of Aspergillus japonicus with high potential of metals
biosorption was selected for further research during this work. Soil analysis revealed that lead concentration was
5.25 mg/ l. The aims of the present study were to gamma irradiate A. japonicus with low doses of 50 to 250Gy, in
order to enhance its heavy metal tolerance ability, and to increase its efficacy for removal of lead (Pb) from the
soil. Results indicated that combined treatments of A. japonicus with lead and gamma irradiation doses displayed
more enhancements of its biosorption capacity and gain in dry biomass than single treatment. Treatment with Pb
(1950 mg/ l) in combination with gamma irradiation (100 Gy) proved to be optimum for increasing the
biosorption capacity of this isolate. However, Pb at 650 mg/ l combined with gamma irradiation at 100 Gy was
the optimum for gain in dry biomass. Fourier Transform Infrared Spectroscopic (FTIR) analysis showed that the
fungal biomass includes in its surface hydroxyl, carboxyl and amine groups. On the other hand, Transmission
electron microscope (TEM) examination of the irradiated A. japonicus cells demonstrated accumulation of
electron dense Pb on the cell wall and within the fungal cells, this indicate that Pb2+ accumulation by fungi occurs
by two methods absorption inside the cell and adsorption on the cell wall. The current study expressed a potential
new method for enhancing microbial biosorption using low doses of gamma radiation. |
| first_indexed | 2024-04-24T08:20:56Z |
| format | Article |
| id | doaj.art-ed7bbdea564444b893b8f6194d372e9c |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:20:56Z |
| publishDate | 2020-10-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-ed7bbdea564444b893b8f6194d372e9c2024-04-17T01:38:54ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942020-10-014597999110.21608/nrmj.2020.118448Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicusHosam, El-Sayyad0Khaled, F. Elyasergy1Tahany, M. Abdel Rahaman2Moustafa, A.S. Aly3Menna Allah, Ashraf4Middle Eastern Regional Radioisotope Centre for the Arab Countries, Dokki12311, Giza, Egypt; Clinical Laboratory Sciences, Al-Ghad International College for Applied Medical Sciences Dammam, Kingdom of Saudi ArabiaMicrobiology Department, Faculty of Science, Cairo University, EgyptMicrobiology Department, Faculty of Science, Cairo University, EgyptRadioisotope Department, Nuclear Research Center, Egyptian, Atomic Energy Authority, EgyptMiddle Eastern Regional Radioisotope Centre for the Arab Countries, Dokki12311, Giza, EgyptAmong the different types of microorganisms; the fungal biomass is considered as an effective mediator for bioremediation of heavy metals, due to its higher surface area and extensive hyphal density in the soil. About seven fungal spp. were isolated from the soil garden of the Middle Eastern Regional Radioisotope Center for Arab Countries (MERRCAC); however, a single isolate of Aspergillus japonicus with high potential of metals biosorption was selected for further research during this work. Soil analysis revealed that lead concentration was 5.25 mg/ l. The aims of the present study were to gamma irradiate A. japonicus with low doses of 50 to 250Gy, in order to enhance its heavy metal tolerance ability, and to increase its efficacy for removal of lead (Pb) from the soil. Results indicated that combined treatments of A. japonicus with lead and gamma irradiation doses displayed more enhancements of its biosorption capacity and gain in dry biomass than single treatment. Treatment with Pb (1950 mg/ l) in combination with gamma irradiation (100 Gy) proved to be optimum for increasing the biosorption capacity of this isolate. However, Pb at 650 mg/ l combined with gamma irradiation at 100 Gy was the optimum for gain in dry biomass. Fourier Transform Infrared Spectroscopic (FTIR) analysis showed that the fungal biomass includes in its surface hydroxyl, carboxyl and amine groups. On the other hand, Transmission electron microscope (TEM) examination of the irradiated A. japonicus cells demonstrated accumulation of electron dense Pb on the cell wall and within the fungal cells, this indicate that Pb2+ accumulation by fungi occurs by two methods absorption inside the cell and adsorption on the cell wall. The current study expressed a potential new method for enhancing microbial biosorption using low doses of gamma radiation.https://nrmj.journals.ekb.eg/article_118448_e527a168126e39b893c5f38b8f8d507c.pdffungiaspergillus japonicusgamma radiationleadftirtem |
| spellingShingle | Hosam, El-Sayyad Khaled, F. Elyasergy Tahany, M. Abdel Rahaman Moustafa, A.S. Aly Menna Allah, Ashraf Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus Novel Research in Microbiology Journal fungi aspergillus japonicus gamma radiation lead ftir tem |
| title | Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus |
| title_full | Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus |
| title_fullStr | Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus |
| title_full_unstemmed | Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus |
| title_short | Enhancement of lead (Pb) biosorption by Gamma irradiated Aspergillus japonicus |
| title_sort | enhancement of lead pb biosorption by gamma irradiated aspergillus japonicus |
| topic | fungi aspergillus japonicus gamma radiation lead ftir tem |
| url | https://nrmj.journals.ekb.eg/article_118448_e527a168126e39b893c5f38b8f8d507c.pdf |
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