Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids
Catharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbace...
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| Format: | Article |
| Language: | English |
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Iranian Medicinal Plants Society
2018-09-01
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| Series: | Journal of Medicinal Plants and By-products |
| Subjects: | |
| Online Access: | http://jmpb.areeo.ac.ir/article_116733_8c6d7df6a0066c453d46d2afdb22a4c8.pdf |
| _version_ | 1811218126313881600 |
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| author | Babak Delnavaz Hashemloian Azra Ataei Azimi |
| author_facet | Babak Delnavaz Hashemloian Azra Ataei Azimi |
| author_sort | Babak Delnavaz Hashemloian |
| collection | DOAJ |
| description | Catharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbacea, Catharanthus roseus andcolchicineon mitosis and microtubule arrangement of human blood cells. In this research, alkaloids were extracted from V. herbacea (herba)and C. roseus (rose). In vitro anti-mitotic and microtubule shortening effects of different concentration of these alkaloids and colchicine were studied on peripheral blood cells. Three alkaloids include herba alkaloid (V. herbacea alkaloid) and rose alkaloid (C. roseus alkaloid) and colchicine, especially herba alkaloid with increasing concentrations (0, 5, 10 and 20 µg.ml-1) that induced mitotic block at the metaphase to anaphase transition. Mitotically blocked cells were exhibited aberrant spindles by microtubule dynamics suppression. All three alkaloids strongly suppresses the rate and extent of microtubule shortening in vitro. Our results showed V. herbacea are a novel source of anti-mitotic and anticancer compounds, probably better of C. roseus. |
| first_indexed | 2024-04-12T07:04:18Z |
| format | Article |
| id | doaj.art-ed7ca264a7de4a58a8fdbb154475c46a |
| institution | Directory Open Access Journal |
| issn | 2322-1399 2588-3739 |
| language | English |
| last_indexed | 2024-04-12T07:04:18Z |
| publishDate | 2018-09-01 |
| publisher | Iranian Medicinal Plants Society |
| record_format | Article |
| series | Journal of Medicinal Plants and By-products |
| spelling | doaj.art-ed7ca264a7de4a58a8fdbb154475c46a2022-12-22T03:42:54ZengIranian Medicinal Plants SocietyJournal of Medicinal Plants and By-products2322-13992588-37392018-09-01718390116733Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine AlkaloidsBabak Delnavaz Hashemloian0Azra Ataei Azimi1Department of plant Cell Biology, Saveh branch, Islamic Azad University, Saveh, IranDepartment of plant Cell Biology, Saveh branch, Islamic Azad University, Saveh, IranCatharanthus roseus (L.) G.Don is a plant which produces anticancer and anti-mitotic indole alkaloids. Colchicine is an anti-mitotic drug. Anti-mitotic effects of Vinca herbacea Waldst. & Kit. indole alkaloids is unknown. The study were evaluated the antimitotic effect of alkaloids of V. herbacea, Catharanthus roseus andcolchicineon mitosis and microtubule arrangement of human blood cells. In this research, alkaloids were extracted from V. herbacea (herba)and C. roseus (rose). In vitro anti-mitotic and microtubule shortening effects of different concentration of these alkaloids and colchicine were studied on peripheral blood cells. Three alkaloids include herba alkaloid (V. herbacea alkaloid) and rose alkaloid (C. roseus alkaloid) and colchicine, especially herba alkaloid with increasing concentrations (0, 5, 10 and 20 µg.ml-1) that induced mitotic block at the metaphase to anaphase transition. Mitotically blocked cells were exhibited aberrant spindles by microtubule dynamics suppression. All three alkaloids strongly suppresses the rate and extent of microtubule shortening in vitro. Our results showed V. herbacea are a novel source of anti-mitotic and anticancer compounds, probably better of C. roseus.http://jmpb.areeo.ac.ir/article_116733_8c6d7df6a0066c453d46d2afdb22a4c8.pdfAntimititicSpidleMicrotubuleAnticancer |
| spellingShingle | Babak Delnavaz Hashemloian Azra Ataei Azimi Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids Journal of Medicinal Plants and By-products Antimititic Spidle Microtubule Anticancer |
| title | Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids |
| title_full | Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids |
| title_fullStr | Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids |
| title_full_unstemmed | Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids |
| title_short | Mitotic Block of Human Blood Cells by Vinca herbacea, Catharanthus roseus and Colchicine Alkaloids |
| title_sort | mitotic block of human blood cells by vinca herbacea catharanthus roseus and colchicine alkaloids |
| topic | Antimititic Spidle Microtubule Anticancer |
| url | http://jmpb.areeo.ac.ir/article_116733_8c6d7df6a0066c453d46d2afdb22a4c8.pdf |
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