Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli
AIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We ha...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340917300872 |
| _version_ | 1818486695204487168 |
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| author | Roshan Jha Hye Young Cho Ameeq Ul Mushtaq Kiho Lee Dae Gyu Kim Sunghoon Kim Young Ho Jeon |
| author_facet | Roshan Jha Hye Young Cho Ameeq Ul Mushtaq Kiho Lee Dae Gyu Kim Sunghoon Kim Young Ho Jeon |
| author_sort | Roshan Jha |
| collection | DOAJ |
| description | AIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We have shown, here, the data for the expression of AIMP2-DX2 protein in Escherichia coli and optimization of the critical steps in purification of AIMP2-DX2. The data described here has been successfully used to get a maximum yield of highly pure AIMP2-DX2 for subsequent characterization of its biophysical property in: “Purification and biophysical characterization of the AIMP2-DX2 protein” (R. Jha, H.Y. Cho, A. Ul Mushtaq, K. Lee, D.G. Kim, S. Kim, et al., 2017) [3]. |
| first_indexed | 2024-12-10T16:26:32Z |
| format | Article |
| id | doaj.art-ed7d5102d0074826bd1dbd6b0852f647 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-10T16:26:32Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-ed7d5102d0074826bd1dbd6b0852f6472022-12-22T01:41:40ZengElsevierData in Brief2352-34092017-04-0111C53353610.1016/j.dib.2017.03.011Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coliRoshan Jha0Hye Young Cho1Ameeq Ul Mushtaq2Kiho Lee3Dae Gyu Kim4Sunghoon Kim5Young Ho Jeon6College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of KoreaCollege of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of KoreaCollege of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of KoreaCollege of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of KoreaMedicinal Bioconvergence Research Center, Seoul National University, Seoul 08826, Republic of KoreaMedicinal Bioconvergence Research Center, Seoul National University, Seoul 08826, Republic of KoreaCollege of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of KoreaAIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We have shown, here, the data for the expression of AIMP2-DX2 protein in Escherichia coli and optimization of the critical steps in purification of AIMP2-DX2. The data described here has been successfully used to get a maximum yield of highly pure AIMP2-DX2 for subsequent characterization of its biophysical property in: “Purification and biophysical characterization of the AIMP2-DX2 protein” (R. Jha, H.Y. Cho, A. Ul Mushtaq, K. Lee, D.G. Kim, S. Kim, et al., 2017) [3].http://www.sciencedirect.com/science/article/pii/S2352340917300872AIMP2AIMP2-DX2Expression and solubilityProtein stabilizersSUMO-tag |
| spellingShingle | Roshan Jha Hye Young Cho Ameeq Ul Mushtaq Kiho Lee Dae Gyu Kim Sunghoon Kim Young Ho Jeon Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli Data in Brief AIMP2 AIMP2-DX2 Expression and solubility Protein stabilizers SUMO-tag |
| title | Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli |
| title_full | Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli |
| title_fullStr | Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli |
| title_full_unstemmed | Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli |
| title_short | Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli |
| title_sort | data on optimization of expression and purification of aimp2 dx2 protein in escherichia coli |
| topic | AIMP2 AIMP2-DX2 Expression and solubility Protein stabilizers SUMO-tag |
| url | http://www.sciencedirect.com/science/article/pii/S2352340917300872 |
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