Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan
Probiotics are dietary supplements of live microorganisms which when consumed in adequate amounts, can have a beneficial effect on the host. Among all bacteria, lactic acid bacteria are the most common type that has been introduced as probiotics. These bacteria are present in dairy products and prod...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University, Tabriz Branch
2013-11-01
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| Series: | Bihdāsht-i Mavādd-i Ghaz̠āyī |
| Subjects: | |
| Online Access: | http://jfh.iaut.ac.ir/article_517670_b9b30014225efed20ab09f26876730ab.pdf |
| _version_ | 1819021584737763328 |
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| author | T Narimani A Tarinejad M.A Hejazi |
| author_facet | T Narimani A Tarinejad M.A Hejazi |
| author_sort | T Narimani |
| collection | DOAJ |
| description | Probiotics are dietary supplements of live microorganisms which when consumed in adequate amounts, can have a beneficial effect on the host. Among all bacteria, lactic acid bacteria are the most common type that has been introduced as probiotics. These bacteria are present in dairy products and produce lactic acid during the fermentation process. The aim of this study was to isolate and identify the probiotics from microbial flora of milk and traditional yogurt in Kaleibar, Heris and Varzaghan areas. In this study, lactic acid bacteria were isolated by culture and identified based on biochemical properties and resistant to stomach acid and bile salts were evaluated. Then, for more accurate identification of the isolates, the 16S rRNA genes of Lactobacilli were amplified with specific primers and the purified PCR product was sent for sequencing. According to our results, 17 strains of Lactobacilli and 6 strains of Enterococci were reported in Kaleibar, Heris and Varzaghan areas which could be a good candidate for further investigation as probiotic. |
| first_indexed | 2024-12-21T04:09:26Z |
| format | Article |
| id | doaj.art-ed7fc61481394d0fbfd1d4402416cf8f |
| institution | Directory Open Access Journal |
| issn | 2228-7647 2476-6968 |
| language | fas |
| last_indexed | 2024-12-21T04:09:26Z |
| publishDate | 2013-11-01 |
| publisher | Islamic Azad University, Tabriz Branch |
| record_format | Article |
| series | Bihdāsht-i Mavādd-i Ghaz̠āyī |
| spelling | doaj.art-ed7fc61481394d0fbfd1d4402416cf8f2022-12-21T19:16:30ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682013-11-0133 (11) پاییز2337517670Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and VarzaghanT Narimani0A Tarinejad1M.A Hejazi2MSc Student, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran- Associate Professor, Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.- Agricultural Biotechnology Research Institute of Iran, Branch of North-West and West region, Tabriz, Iran.Probiotics are dietary supplements of live microorganisms which when consumed in adequate amounts, can have a beneficial effect on the host. Among all bacteria, lactic acid bacteria are the most common type that has been introduced as probiotics. These bacteria are present in dairy products and produce lactic acid during the fermentation process. The aim of this study was to isolate and identify the probiotics from microbial flora of milk and traditional yogurt in Kaleibar, Heris and Varzaghan areas. In this study, lactic acid bacteria were isolated by culture and identified based on biochemical properties and resistant to stomach acid and bile salts were evaluated. Then, for more accurate identification of the isolates, the 16S rRNA genes of Lactobacilli were amplified with specific primers and the purified PCR product was sent for sequencing. According to our results, 17 strains of Lactobacilli and 6 strains of Enterococci were reported in Kaleibar, Heris and Varzaghan areas which could be a good candidate for further investigation as probiotic.http://jfh.iaut.ac.ir/article_517670_b9b30014225efed20ab09f26876730ab.pdfEnterococciProbiotic Bacteria16S rRNA genelactobacilli |
| spellingShingle | T Narimani A Tarinejad M.A Hejazi Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan Bihdāsht-i Mavādd-i Ghaz̠āyī Enterococci Probiotic Bacteria 16S rRNA gene lactobacilli |
| title | Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan |
| title_full | Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan |
| title_fullStr | Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan |
| title_full_unstemmed | Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan |
| title_short | Isolation and identification of lactic acid bacteria from traditional dairy products of Kleibar, Heris and Varzaghan |
| title_sort | isolation and identification of lactic acid bacteria from traditional dairy products of kleibar heris and varzaghan |
| topic | Enterococci Probiotic Bacteria 16S rRNA gene lactobacilli |
| url | http://jfh.iaut.ac.ir/article_517670_b9b30014225efed20ab09f26876730ab.pdf |
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