Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure
<i>Panax ginseng</i> is a well-known medicinal plant that achieves strong resistance against plant pathogens while growing in the wild. Due to the high market demand for ginseng as a health food source, ginseng cultivation is prevalent in South Korea. However, continuous monocropping cre...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/11/2177 |
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| author | Jerald-Conrad Ibal Min-Kyu Park Gun-Seok Park Byung-Kwon Jung Tae-Hyung Park Min-Sueng Kim Gi-Ung Kang Yeong-Jun Park Jae-Ho Shin |
| author_facet | Jerald-Conrad Ibal Min-Kyu Park Gun-Seok Park Byung-Kwon Jung Tae-Hyung Park Min-Sueng Kim Gi-Ung Kang Yeong-Jun Park Jae-Ho Shin |
| author_sort | Jerald-Conrad Ibal |
| collection | DOAJ |
| description | <i>Panax ginseng</i> is a well-known medicinal plant that achieves strong resistance against plant pathogens while growing in the wild. Due to the high market demand for ginseng as a health food source, ginseng cultivation is prevalent in South Korea. However, continuous monocropping creates problems like irregular growth or vulnerability to crop diseases. Quorum sensing (QS) deals with the intracellular communication of bacteria and plays a role in dynamic changes in the soil microbiome. Here, we investigated how acyl-homoserine lactone (AHL) signaling molecules in QS (C8, C10, and C12) improve plant growth and induce shifts in the soil microbiome. To assess the effects, we recorded root and shoot growth of ginseng seedlings and checked the changes in the soil microbiome during different time points (0, 2, 4, and 8) after 8 weeks of growth. We observed that soils treated with <i>N</i>-decanoyl-<sub>L</sub>-homoserine lactone (C10) showed the most pronounced effects. Very striking was that C10 had the lowest alpha diversity. Using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2), we observed a high number of QS-related functional genes, with the highest count occurring in the untreated planted soil (W). Together with the known direct and beneficial effects of AHLs on plant development, AHLs treated mono-cropped soil showed trends in the microbiome community. |
| first_indexed | 2024-03-10T05:47:36Z |
| format | Article |
| id | doaj.art-ed80abbe8e0342ecbcae65582b4d1f6b |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:47:36Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-ed80abbe8e0342ecbcae65582b4d1f6b2023-11-22T22:01:41ZengMDPI AGAgronomy2073-43952021-10-011111217710.3390/agronomy11112177Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome StructureJerald-Conrad Ibal0Min-Kyu Park1Gun-Seok Park2Byung-Kwon Jung3Tae-Hyung Park4Min-Sueng Kim5Gi-Ung Kang6Yeong-Jun Park7Jae-Ho Shin8Department of Applied Biosciences, Kyungpook National University, Daehak-ro 80, Daegu 41566, KoreaDepartment of Applied Biosciences, Kyungpook National University, Daehak-ro 80, Daegu 41566, KoreaAtogen Co., Ltd., Yuseong-gu, Daejeon 34015, KoreaCJ Blossom Park, Yeongtong-gu, Suwon-si 16495, KoreaDepartment of Integrative Biotechnology, Kyungpook National University, Daehak-ro 80, Daegu 41566, KoreaDepartment of Integrative Biotechnology, Kyungpook National University, Daehak-ro 80, Daegu 41566, KoreaDeutsches Rheuma-Forschungszentrum (DRFZ), Institute of the Leibniz Association, 10117 Berlin, GermanyDepartment of Applied Biosciences, Kyungpook National University, Daehak-ro 80, Daegu 41566, KoreaDepartment of Applied Biosciences, Kyungpook National University, Daehak-ro 80, Daegu 41566, Korea<i>Panax ginseng</i> is a well-known medicinal plant that achieves strong resistance against plant pathogens while growing in the wild. Due to the high market demand for ginseng as a health food source, ginseng cultivation is prevalent in South Korea. However, continuous monocropping creates problems like irregular growth or vulnerability to crop diseases. Quorum sensing (QS) deals with the intracellular communication of bacteria and plays a role in dynamic changes in the soil microbiome. Here, we investigated how acyl-homoserine lactone (AHL) signaling molecules in QS (C8, C10, and C12) improve plant growth and induce shifts in the soil microbiome. To assess the effects, we recorded root and shoot growth of ginseng seedlings and checked the changes in the soil microbiome during different time points (0, 2, 4, and 8) after 8 weeks of growth. We observed that soils treated with <i>N</i>-decanoyl-<sub>L</sub>-homoserine lactone (C10) showed the most pronounced effects. Very striking was that C10 had the lowest alpha diversity. Using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2), we observed a high number of QS-related functional genes, with the highest count occurring in the untreated planted soil (W). Together with the known direct and beneficial effects of AHLs on plant development, AHLs treated mono-cropped soil showed trends in the microbiome community.https://www.mdpi.com/2073-4395/11/11/2177ginsengsoil microbiomequorum sensing |
| spellingShingle | Jerald-Conrad Ibal Min-Kyu Park Gun-Seok Park Byung-Kwon Jung Tae-Hyung Park Min-Sueng Kim Gi-Ung Kang Yeong-Jun Park Jae-Ho Shin Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure Agronomy ginseng soil microbiome quorum sensing |
| title | Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure |
| title_full | Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure |
| title_fullStr | Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure |
| title_full_unstemmed | Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure |
| title_short | Use of Acyl-Homoserine Lactones Leads to Improved Growth of Ginseng Seedlings and Shifts in Soil Microbiome Structure |
| title_sort | use of acyl homoserine lactones leads to improved growth of ginseng seedlings and shifts in soil microbiome structure |
| topic | ginseng soil microbiome quorum sensing |
| url | https://www.mdpi.com/2073-4395/11/11/2177 |
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