One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16

Summary: Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-...

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Bibliographic Details
Main Authors: Sophia Magkouta, Dimitris Veroutis, Athanasios Pousias, Angelos Papaspyropoulos, Kety Giannetti, Natassa Pippa, Nikolaos Lougiakis, Konstantinos Kambas, Nefeli Lagopati, Aikaterini Polyzou, Maria Georgiou, Maria Chountoulesi, Stergios Pispas, Spyros Foutadakis, Efthymios Kyrodimos, Nicole Pouli, Panagiotis Marakos, Athanassios Kotsinas, Panayotis Verginis, Dimitrios Valakos, Giannis Vatsellas, Russell Petty, Dimitris Thanos, Marco Demaria, Konstantinos Evangelou, Raffaella Di Micco, Vassilis G. Gorgoulis
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Cell-based Assays
Flow Cytometry
Cancer
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000947
Description
Summary:Summary: Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present.For complete details on the use and execution of this protocol, please refer to Magkouta et al.1
ISSN:2666-1667

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