Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line
High blood cholesterol levels may increase the risk of developing atherosclerosis. Since intestinal cholesterol absorption plays a major role in maintaining total body cholesterol homeostasis, the aim of the present study was to compare the effect of ezetimibe and three hydroxycinnamic acid derivati...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Pharmacology and Drug Discovery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590257122000256 |
| _version_ | 1811178283695341568 |
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| author | Asma Ressaissi Maria Luísa M. Serralheiro |
| author_facet | Asma Ressaissi Maria Luísa M. Serralheiro |
| author_sort | Asma Ressaissi |
| collection | DOAJ |
| description | High blood cholesterol levels may increase the risk of developing atherosclerosis. Since intestinal cholesterol absorption plays a major role in maintaining total body cholesterol homeostasis, the aim of the present study was to compare the effect of ezetimibe and three hydroxycinnamic acid derivatives (rosmarinic acid, chlorogenic acid and m-coumaric acid) that are present in several medicinal plants on cholesterol absorption in the intestinal Caco-2 cells. In addition to the permeability assays, studies on alteration of the biochemical properties of Caco-2 cells under the effect of ezetimibe and hydroxycinnamic acid derivatives was evaluated using FTIR accompanied with multivariate analysis by PCA. The cholesterol permeability assays showed that these compounds could decrease cholesterol permeability with a percentage ranging from 76.98 to 96.6% with the highest inhibition for ezetimibe. whereas the FTIR studies didn't show similar changes between ezetimibe and the three hydroxycinnamic acid derivatives in protein and nucleic acids region, suggesting that these compounds have hypocholesterolemic effect. Nevertheless, each compound originated a different change on Caco-2 treated cells suggesting a different mode of action. |
| first_indexed | 2024-04-11T06:15:50Z |
| format | Article |
| id | doaj.art-ed8313fe074c43b58178b793376bbd2d |
| institution | Directory Open Access Journal |
| issn | 2590-2571 |
| language | English |
| last_indexed | 2024-04-11T06:15:50Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Pharmacology and Drug Discovery |
| spelling | doaj.art-ed8313fe074c43b58178b793376bbd2d2022-12-22T04:41:03ZengElsevierCurrent Research in Pharmacology and Drug Discovery2590-25712022-01-013100105Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell lineAsma Ressaissi0Maria Luísa M. Serralheiro1Universidade de Lisboa, Faculdade de Ciências, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, C8, 1749-016, Lisboa, Portugal; Corresponding author.Universidade de Lisboa, Faculdade de Ciências, BioISI - Biosystems & Integrative Sciences Institute, Campo Grande, C8, 1749-016, Lisboa, Portugal; Universidade de Lisboa, Faculdade de Ciências, Departamento de Química e Bioquímica, Campo Grande, 1749-016, Lisboa, PortugalHigh blood cholesterol levels may increase the risk of developing atherosclerosis. Since intestinal cholesterol absorption plays a major role in maintaining total body cholesterol homeostasis, the aim of the present study was to compare the effect of ezetimibe and three hydroxycinnamic acid derivatives (rosmarinic acid, chlorogenic acid and m-coumaric acid) that are present in several medicinal plants on cholesterol absorption in the intestinal Caco-2 cells. In addition to the permeability assays, studies on alteration of the biochemical properties of Caco-2 cells under the effect of ezetimibe and hydroxycinnamic acid derivatives was evaluated using FTIR accompanied with multivariate analysis by PCA. The cholesterol permeability assays showed that these compounds could decrease cholesterol permeability with a percentage ranging from 76.98 to 96.6% with the highest inhibition for ezetimibe. whereas the FTIR studies didn't show similar changes between ezetimibe and the three hydroxycinnamic acid derivatives in protein and nucleic acids region, suggesting that these compounds have hypocholesterolemic effect. Nevertheless, each compound originated a different change on Caco-2 treated cells suggesting a different mode of action.http://www.sciencedirect.com/science/article/pii/S2590257122000256CholesterolHydroxycinnamic acid derivativesEzetimibeFTIRPermeabilityPCA |
| spellingShingle | Asma Ressaissi Maria Luísa M. Serralheiro Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line Current Research in Pharmacology and Drug Discovery Cholesterol Hydroxycinnamic acid derivatives Ezetimibe FTIR Permeability PCA |
| title | Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line |
| title_full | Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line |
| title_fullStr | Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line |
| title_full_unstemmed | Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line |
| title_short | Hydroxycinnamic acid derivatives effect on hypercholesterolemia, comparison with ezetimibe: Permeability assays and FTIR spectroscopy on Caco-2 cell line |
| title_sort | hydroxycinnamic acid derivatives effect on hypercholesterolemia comparison with ezetimibe permeability assays and ftir spectroscopy on caco 2 cell line |
| topic | Cholesterol Hydroxycinnamic acid derivatives Ezetimibe FTIR Permeability PCA |
| url | http://www.sciencedirect.com/science/article/pii/S2590257122000256 |
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