Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease
Kawasaki disease (KD) is a common systemic vasculitides in children younger than 5 years of age. Activated macrophages are key drivers of vascular inflammation in KD. The aim of this study was to examine differences in M1 and M2 macrophage marker expression in patients with KD. Blood samples were ob...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Pediatrics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fped.2020.00129/full |
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| author | Mindy Ming-Huey Guo Mindy Ming-Huey Guo Mindy Ming-Huey Guo Ling-Sai Chang Ling-Sai Chang Ying-Hsien Huang Ying-Hsien Huang Feng-Sheng Wang Feng-Sheng Wang Feng-Sheng Wang Ho-Chang Kuo Ho-Chang Kuo Ho-Chang Kuo |
| author_facet | Mindy Ming-Huey Guo Mindy Ming-Huey Guo Mindy Ming-Huey Guo Ling-Sai Chang Ling-Sai Chang Ying-Hsien Huang Ying-Hsien Huang Feng-Sheng Wang Feng-Sheng Wang Feng-Sheng Wang Ho-Chang Kuo Ho-Chang Kuo Ho-Chang Kuo |
| author_sort | Mindy Ming-Huey Guo |
| collection | DOAJ |
| description | Kawasaki disease (KD) is a common systemic vasculitides in children younger than 5 years of age. Activated macrophages are key drivers of vascular inflammation in KD. The aim of this study was to examine differences in M1 and M2 macrophage marker expression in patients with KD. Blood samples were obtained from 18 healthy controls and 18 patients with KD at 24 h prior and 21 days after to intravenous immunoglobulin therapy. GeneChip Human Transcriptome Array 2.0 and Illumina HumanMethylation450 BeadChip were used to examined the mRNA expression and corresponding CpG site methylation ratios of 10 M1 surface markers and 15 M2 surface markers. Of the markers examined 2 M1 markers (TLR2, IL2RA) and 8 M2 markers (ARG1, CCR2, TLR1, TLR8, TLR5, MS4A6A, CD36, and MS4A4A) showed increased mRNA expression in the acute phase of KD which decreased after IVIG therapy (P < 0.05). Corresponding CpG sites in the promoter regions these markers were hypomethylated in the acute phase of KD and significantly increased after IVIG therapy. In conclusion, both M1 and M2 markers showed increased mRNA expression in the acute phase of KD. CpG site methylation may be one of the mechanisms governing macrophage polarization in KD. |
| first_indexed | 2024-12-22T08:40:26Z |
| format | Article |
| id | doaj.art-ed831e49b5084aae90c27c6ccb09a5fc |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-12-22T08:40:26Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-ed831e49b5084aae90c27c6ccb09a5fc2022-12-21T18:32:15ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602020-04-01810.3389/fped.2020.00129499847Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki DiseaseMindy Ming-Huey Guo0Mindy Ming-Huey Guo1Mindy Ming-Huey Guo2Ling-Sai Chang3Ling-Sai Chang4Ying-Hsien Huang5Ying-Hsien Huang6Feng-Sheng Wang7Feng-Sheng Wang8Feng-Sheng Wang9Ho-Chang Kuo10Ho-Chang Kuo11Ho-Chang Kuo12Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanGraduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kaohsiung City, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanGraduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kaohsiung City, TaiwanDepartment of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanCore Laboratory for Phenomics & Diagnostics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University, College of Medicine, Kaohsiung, TaiwanGraduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Kaohsiung City, TaiwanKawasaki disease (KD) is a common systemic vasculitides in children younger than 5 years of age. Activated macrophages are key drivers of vascular inflammation in KD. The aim of this study was to examine differences in M1 and M2 macrophage marker expression in patients with KD. Blood samples were obtained from 18 healthy controls and 18 patients with KD at 24 h prior and 21 days after to intravenous immunoglobulin therapy. GeneChip Human Transcriptome Array 2.0 and Illumina HumanMethylation450 BeadChip were used to examined the mRNA expression and corresponding CpG site methylation ratios of 10 M1 surface markers and 15 M2 surface markers. Of the markers examined 2 M1 markers (TLR2, IL2RA) and 8 M2 markers (ARG1, CCR2, TLR1, TLR8, TLR5, MS4A6A, CD36, and MS4A4A) showed increased mRNA expression in the acute phase of KD which decreased after IVIG therapy (P < 0.05). Corresponding CpG sites in the promoter regions these markers were hypomethylated in the acute phase of KD and significantly increased after IVIG therapy. In conclusion, both M1 and M2 markers showed increased mRNA expression in the acute phase of KD. CpG site methylation may be one of the mechanisms governing macrophage polarization in KD.https://www.frontiersin.org/article/10.3389/fped.2020.00129/fullKawasaki diseasemacrophagemethylationtranscriptome arraymethylation array |
| spellingShingle | Mindy Ming-Huey Guo Mindy Ming-Huey Guo Mindy Ming-Huey Guo Ling-Sai Chang Ling-Sai Chang Ying-Hsien Huang Ying-Hsien Huang Feng-Sheng Wang Feng-Sheng Wang Feng-Sheng Wang Ho-Chang Kuo Ho-Chang Kuo Ho-Chang Kuo Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease Frontiers in Pediatrics Kawasaki disease macrophage methylation transcriptome array methylation array |
| title | Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease |
| title_full | Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease |
| title_fullStr | Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease |
| title_full_unstemmed | Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease |
| title_short | Epigenetic Regulation of Macrophage Marker Expression Profiles in Kawasaki Disease |
| title_sort | epigenetic regulation of macrophage marker expression profiles in kawasaki disease |
| topic | Kawasaki disease macrophage methylation transcriptome array methylation array |
| url | https://www.frontiersin.org/article/10.3389/fped.2020.00129/full |
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