Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion
BackgroundOral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modificatio...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1013054/full |
| _version_ | 1811175371969658880 |
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| author | Zhiming Qin Zhiming Qin Jiaying Bai Jiaying Bai Huiying He Binbin Li Binbin Li |
| author_facet | Zhiming Qin Zhiming Qin Jiaying Bai Jiaying Bai Huiying He Binbin Li Binbin Li |
| author_sort | Zhiming Qin |
| collection | DOAJ |
| description | BackgroundOral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modification of both coding mRNA and non-coding ncRNA in eukaryotes, participates in the regulation of the occurrence and development of various malignant tumors in human. However, its role in oral epithelial dysplasia (OED) and OSCC remain unclear.Materials and methodsIn this study, multiple public databases were used for bioinformatics analysis of 23 common m6A methylation regulators in head and neck squamous cell carcinoma (HNSCC). Protein expressions of IGF2BP2 and IGF2BP3 were verified accordingly in clinical cohort samples of OED and OSCC.ResultsPatients with high expression of FTO、HNRNPC、HNRNPA2B1、LRPPRC、IGF2BP1、IGF2BP2、IGF2BP3 had a poor prognosis. IGF2BP2 had a relatively high mutation rate in HNSCC, and its expression was significantly positively correlated with tumor purity, and significantly negatively correlated with the infiltration level of B cells and CD8+T cells. The expression of IGF2BP3 was significantly positively correlated with tumor purity and CD4+T cells. Immunohistochemistrically, the expression of IGF2BP2 and IGF2BP3 in oral simple epithelial hyperplasia, OED and OSCC increased gradually. Both were strongly expressed in OSCC.ConclusionIGF2BP2 and IGF2BP3 were the potential biological prognostic indicators of OED and OSCC. |
| first_indexed | 2024-04-10T19:35:02Z |
| format | Article |
| id | doaj.art-ed84c8956724405f95cffef50c7c83ce |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-10T19:35:02Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-ed84c8956724405f95cffef50c7c83ce2023-01-30T08:13:23ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-01-011310.3389/fonc.2023.10130541013054Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesionZhiming Qin0Zhiming Qin1Jiaying Bai2Jiaying Bai3Huiying He4Binbin Li5Binbin Li6Department of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaResearch Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, ChinaDepartment of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaResearch Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, ChinaDepartment of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University Health Science Center, Beijing, ChinaDepartment of Oral Pathology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaResearch Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, ChinaBackgroundOral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC) are a series of related pathologic and molecular events involving simple epithelial hyperplasia, mild to severe dysplasia and canceration. N6-methyladenosine RNA methylation, as the most common modification of both coding mRNA and non-coding ncRNA in eukaryotes, participates in the regulation of the occurrence and development of various malignant tumors in human. However, its role in oral epithelial dysplasia (OED) and OSCC remain unclear.Materials and methodsIn this study, multiple public databases were used for bioinformatics analysis of 23 common m6A methylation regulators in head and neck squamous cell carcinoma (HNSCC). Protein expressions of IGF2BP2 and IGF2BP3 were verified accordingly in clinical cohort samples of OED and OSCC.ResultsPatients with high expression of FTO、HNRNPC、HNRNPA2B1、LRPPRC、IGF2BP1、IGF2BP2、IGF2BP3 had a poor prognosis. IGF2BP2 had a relatively high mutation rate in HNSCC, and its expression was significantly positively correlated with tumor purity, and significantly negatively correlated with the infiltration level of B cells and CD8+T cells. The expression of IGF2BP3 was significantly positively correlated with tumor purity and CD4+T cells. Immunohistochemistrically, the expression of IGF2BP2 and IGF2BP3 in oral simple epithelial hyperplasia, OED and OSCC increased gradually. Both were strongly expressed in OSCC.ConclusionIGF2BP2 and IGF2BP3 were the potential biological prognostic indicators of OED and OSCC.https://www.frontiersin.org/articles/10.3389/fonc.2023.1013054/fulloral squamous cell carcinomaoral epithelial dysplasiaN6-methyladenosineIGF2BP2IGF2BP3 |
| spellingShingle | Zhiming Qin Zhiming Qin Jiaying Bai Jiaying Bai Huiying He Binbin Li Binbin Li Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion Frontiers in Oncology oral squamous cell carcinoma oral epithelial dysplasia N6-methyladenosine IGF2BP2 IGF2BP3 |
| title | Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion |
| title_full | Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion |
| title_fullStr | Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion |
| title_full_unstemmed | Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion |
| title_short | Expression and significance of m6A-RNA-methylation in oral cancer and precancerous lesion |
| title_sort | expression and significance of m6a rna methylation in oral cancer and precancerous lesion |
| topic | oral squamous cell carcinoma oral epithelial dysplasia N6-methyladenosine IGF2BP2 IGF2BP3 |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1013054/full |
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