Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression
Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2008-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640803355D.pdf |
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| author | Dinić Svetlana Mihailović Mirjana Ivanović-Matić Svetlana Uskoković Aleksandra Grdović Nevena Vidaković Melita Poznanović G. |
| author_facet | Dinić Svetlana Mihailović Mirjana Ivanović-Matić Svetlana Uskoković Aleksandra Grdović Nevena Vidaković Melita Poznanović G. |
| author_sort | Dinić Svetlana |
| collection | DOAJ |
| description | Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences. |
| first_indexed | 2024-12-23T04:01:44Z |
| format | Article |
| id | doaj.art-ed854b80b08c4a25a86009657ca85ecd |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-12-23T04:01:44Z |
| publishDate | 2008-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-ed854b80b08c4a25a86009657ca85ecd2022-12-21T18:00:44ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642008-01-0160335536610.2298/ABS0803355DDynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expressionDinić SvetlanaMihailović MirjanaIvanović-Matić SvetlanaUskoković AleksandraGrdović NevenaVidaković MelitaPoznanović G.Participation of the nuclear matrix in regulation of alpha-2-macroglobulin (α2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the α2M gene promoter (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPα, and STAT5b were involved in basal and C/EBPβ, STAT1, and STAT3 in AP-stimulated α2M expression. These findings support the assumption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640803355D.pdfAlpha-2-macroglobulinacute phase responserat livernuclear matrixtranscription factors |
| spellingShingle | Dinić Svetlana Mihailović Mirjana Ivanović-Matić Svetlana Uskoković Aleksandra Grdović Nevena Vidaković Melita Poznanović G. Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression Archives of Biological Sciences Alpha-2-macroglobulin acute phase response rat liver nuclear matrix transcription factors |
| title | Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression |
| title_full | Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression |
| title_fullStr | Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression |
| title_full_unstemmed | Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression |
| title_short | Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha-2-macroglobulin gene expression |
| title_sort | dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alpha 2 macroglobulin gene expression |
| topic | Alpha-2-macroglobulin acute phase response rat liver nuclear matrix transcription factors |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2008/0354-46640803355D.pdf |
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