Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression
Glioma is a primary intracranial malignant tumor with poor prognosis, and its pathogenesis is unclear. This study discussed the impact of p53/lncRNA plasmacytoma variant translocation 1 (lncRNA PVT1)/transforming growth factor beta (TGF-β)/Smad axis on the biological characteristics of glioma. Gliom...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Cancer Biology & Therapy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15384047.2022.2042160 |
| _version_ | 1797404083309510656 |
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| author | Zhang Li Ming Li Pengcheng Xia Lili Wang Zhiming Lu |
| author_facet | Zhang Li Ming Li Pengcheng Xia Lili Wang Zhiming Lu |
| author_sort | Zhang Li |
| collection | DOAJ |
| description | Glioma is a primary intracranial malignant tumor with poor prognosis, and its pathogenesis is unclear. This study discussed the impact of p53/lncRNA plasmacytoma variant translocation 1 (lncRNA PVT1)/transforming growth factor beta (TGF-β)/Smad axis on the biological characteristics of glioma. Glioma and normal tissues were collected, in which relative lncRNA PVT1 and p53 expression was assessed. Pearson’s analysis was adopted for the correlation analysis between lncRNA PVT1 and p53. Short interfering RNA (siRNA) against lncRNA PVT1 (siRNA-PVT1), siRNA-p53 or both was transfected into the glioma cells to evaluate effects of lncRNA PVT1 and p53 on cell proliferation, migration, invasion, and apoptosis. Mouse xenograft model of glioma was established to verify function of lncRNA PVT1 and p53 in vivo. Relationship among p53, lncRNA PVT1 and TGF-β/Smad was predicted and confirmed. Glioma tissues and cells showed downregulated p53 expression and increased lncRNA PVT1 expression. An adverse relationship was noted between p53 expression and lncRNA PVT1 expression. p53 was shown to be enriched in the lncRNA PVT1 promoter region and resulted in its suppression. p53 inhibited glioma cell proliferation, migration, and invasion, and induced apoptosis as well as arrested tumor growth by downregulating lncRNA PVT1. LncRNA PVT1was found to bind to TGF-β and activate TGF-β/Smad pathway, promoting progression of glioma. Consequently, p53 exerts anti-oncogenic function on glioma development by suppressing lncRNA PVT1 and subsequently inactivating TGF-β/Smad pathway. |
| first_indexed | 2024-03-09T02:47:51Z |
| format | Article |
| id | doaj.art-ed86be1972054b8da26beaa8c9ad54af |
| institution | Directory Open Access Journal |
| issn | 1538-4047 1555-8576 |
| language | English |
| last_indexed | 2024-03-09T02:47:51Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj.art-ed86be1972054b8da26beaa8c9ad54af2023-12-05T15:58:13ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762022-12-0123122523310.1080/15384047.2022.20421602042160Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progressionZhang Li0Ming Li1Pengcheng Xia2Lili Wang3Zhiming Lu4Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityShandong Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityShandong Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityShandong Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityShandong Provincial Hospital, Cheeloo College of Medicine, Shandong UniversityGlioma is a primary intracranial malignant tumor with poor prognosis, and its pathogenesis is unclear. This study discussed the impact of p53/lncRNA plasmacytoma variant translocation 1 (lncRNA PVT1)/transforming growth factor beta (TGF-β)/Smad axis on the biological characteristics of glioma. Glioma and normal tissues were collected, in which relative lncRNA PVT1 and p53 expression was assessed. Pearson’s analysis was adopted for the correlation analysis between lncRNA PVT1 and p53. Short interfering RNA (siRNA) against lncRNA PVT1 (siRNA-PVT1), siRNA-p53 or both was transfected into the glioma cells to evaluate effects of lncRNA PVT1 and p53 on cell proliferation, migration, invasion, and apoptosis. Mouse xenograft model of glioma was established to verify function of lncRNA PVT1 and p53 in vivo. Relationship among p53, lncRNA PVT1 and TGF-β/Smad was predicted and confirmed. Glioma tissues and cells showed downregulated p53 expression and increased lncRNA PVT1 expression. An adverse relationship was noted between p53 expression and lncRNA PVT1 expression. p53 was shown to be enriched in the lncRNA PVT1 promoter region and resulted in its suppression. p53 inhibited glioma cell proliferation, migration, and invasion, and induced apoptosis as well as arrested tumor growth by downregulating lncRNA PVT1. LncRNA PVT1was found to bind to TGF-β and activate TGF-β/Smad pathway, promoting progression of glioma. Consequently, p53 exerts anti-oncogenic function on glioma development by suppressing lncRNA PVT1 and subsequently inactivating TGF-β/Smad pathway.http://dx.doi.org/10.1080/15384047.2022.2042160p53lncrna pvt1tgf-β/smad pathwaygliomaproliferationapoptosis |
| spellingShingle | Zhang Li Ming Li Pengcheng Xia Lili Wang Zhiming Lu Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression Cancer Biology & Therapy p53 lncrna pvt1 tgf-β/smad pathway glioma proliferation apoptosis |
| title | Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression |
| title_full | Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression |
| title_fullStr | Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression |
| title_full_unstemmed | Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression |
| title_short | Targeting long non-coding RNA PVT1/TGF-β/Smad by p53 prevents glioma progression |
| title_sort | targeting long non coding rna pvt1 tgf β smad by p53 prevents glioma progression |
| topic | p53 lncrna pvt1 tgf-β/smad pathway glioma proliferation apoptosis |
| url | http://dx.doi.org/10.1080/15384047.2022.2042160 |
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