Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR
Classical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmers and pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state and to evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in det...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2018-05-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/79743 |
| _version_ | 1817967816327823360 |
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| author | P CHOORI S S PATIL D RATNAMMA APRAJAPATI S Y MUKARTAL G B MANJUNATHA REDDY K P SURESH D HEMADRI H RAHMAN |
| author_facet | P CHOORI S S PATIL D RATNAMMA APRAJAPATI S Y MUKARTAL G B MANJUNATHA REDDY K P SURESH D HEMADRI H RAHMAN |
| author_sort | P CHOORI |
| collection | DOAJ |
| description | Classical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmers
and pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state and
to evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in detecting the disease. Serum samples
were collected from 270 pigs from 13 districts of the state and were tested for the presence of CSF antibodies.
Whole blood samples (151) from 14 outbreaks of CSF were collected for the comparative diagnosis of the disease
using Antigen ELISA and reverse transcriptase (RT)-PCR. A seroprevalence of 28.5% (77/270) was found in the
serum samples collected form the whole Karnataka. The southern Karnataka had higher seroprevalence (47%) in
comparison to northern Karnataka region where seroprevalence was 17%. It confirms endemicity of the disease in
southern region. Of 151 blood samples collected for comparative diagnosis, 61 samples were positive for CSF by
RT-PCR and 39 by antigen ELISA, indicating the superiority of RT-PCR over antigen ELISA to detect CSFV
infection in earliest stages of infection. |
| first_indexed | 2024-04-10T16:39:30Z |
| format | Article |
| id | doaj.art-ed8a1083bb204da28402e23789b27053 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:39:30Z |
| publishDate | 2018-05-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-ed8a1083bb204da28402e23789b270532023-02-08T11:00:44ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272018-05-01871210.56093/ijans.v87i12.79743Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCRP CHOORI0S S PATIL1D RATNAMMA2APRAJAPATI3S Y MUKARTAL4G B MANJUNATHA REDDY5K P SURESH6D HEMADRI7H RAHMAN8ICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaICAR-NIVEDI, Yelahanka, Bengaluru, Karnataka 560 064 IndiaClassical swine fever is highly contagious viral disease of swine causing huge economic losses to pig farmers and pig industry. The study was conducted in Karnataka to understand the current scenario of CSF in the state and to evaluate efficiency of diagnostic tests (antigen ELISA and RT-PCR) in detecting the disease. Serum samples were collected from 270 pigs from 13 districts of the state and were tested for the presence of CSF antibodies. Whole blood samples (151) from 14 outbreaks of CSF were collected for the comparative diagnosis of the disease using Antigen ELISA and reverse transcriptase (RT)-PCR. A seroprevalence of 28.5% (77/270) was found in the serum samples collected form the whole Karnataka. The southern Karnataka had higher seroprevalence (47%) in comparison to northern Karnataka region where seroprevalence was 17%. It confirms endemicity of the disease in southern region. Of 151 blood samples collected for comparative diagnosis, 61 samples were positive for CSF by RT-PCR and 39 by antigen ELISA, indicating the superiority of RT-PCR over antigen ELISA to detect CSFV infection in earliest stages of infection.https://epubs.icar.org.in/index.php/IJAnS/article/view/79743AntigenAntibodyClassical swine feverELISAKarnatakaRT-PCR |
| spellingShingle | P CHOORI S S PATIL D RATNAMMA APRAJAPATI S Y MUKARTAL G B MANJUNATHA REDDY K P SURESH D HEMADRI H RAHMAN Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR Indian Journal of Animal Sciences Antigen Antibody Classical swine fever ELISA Karnataka RT-PCR |
| title | Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR |
| title_full | Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR |
| title_fullStr | Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR |
| title_full_unstemmed | Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR |
| title_short | Seroprevalence of Classical Swine Fever in pigs of Karnataka and comparative diagnostic evaluation of antigen ELISA and reverse transcriptase -PCR |
| title_sort | seroprevalence of classical swine fever in pigs of karnataka and comparative diagnostic evaluation of antigen elisa and reverse transcriptase pcr |
| topic | Antigen Antibody Classical swine fever ELISA Karnataka RT-PCR |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/79743 |
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