The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture
Yarrowia lipolytica, as a good cell factory to speed up the production of plant pharmaceutical components, has been considered to be one of the most important and attractive micro-organisms in recent years, due to its high secretion capacity, limited glycosylation, large range of genetic markers and...
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| Format: | Article |
| Language: | English |
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Kharazmi University
2020-07-01
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| Series: | یافتههای نوین در علوم زیستی |
| Subjects: | |
| Online Access: | http://nbr.khu.ac.ir/article-1-3298-en.html |
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| author | Monireh Marsafari Habibollah Samizadeh Lahiji Babak Rabiei Ali Ashraf Mehrabi Yongkun Lv Peng Xu |
| author_facet | Monireh Marsafari Habibollah Samizadeh Lahiji Babak Rabiei Ali Ashraf Mehrabi Yongkun Lv Peng Xu |
| author_sort | Monireh Marsafari |
| collection | DOAJ |
| description | Yarrowia lipolytica, as a good cell factory to speed up the production of plant pharmaceutical components, has been considered to be one of the most important and attractive micro-organisms in recent years, due to its high secretion capacity, limited glycosylation, large range of genetic markers and molecular tools. Naringenin, as a central core of flavonoids production, plays important roles both in plants and in the treatment of different types of human diseases. For this purpose, specific naringenin biosynthesis genes from different origins were selected and introduced after comparative expression profiling in Y. lipolytica. This research indicated that chs plays the main role in the production of naringenin, so the increase copy number of this gene in each construct was investigated. The HPLC results confirmed that the construct with 5 copy numbers of chs resulted in 7.14 fold increase of naringenin extracellular titer to 90.16 mg/L in shake flask cultures. The results reported in this study demonstrated that sufficient knowledge of genes involved in the specific biosynthesis pathway, synthetic gene pathway and using Y. lipolytica as a capable and cheap host could help bioengineers to produce significant amounts of pharmaceutical components. |
| first_indexed | 2024-12-12T11:02:21Z |
| format | Article |
| id | doaj.art-ed8a6e7649c94dd3903de5a1b1728ba2 |
| institution | Directory Open Access Journal |
| issn | 2423-6330 2476-7115 |
| language | English |
| last_indexed | 2024-12-12T11:02:21Z |
| publishDate | 2020-07-01 |
| publisher | Kharazmi University |
| record_format | Article |
| series | یافتههای نوین در علوم زیستی |
| spelling | doaj.art-ed8a6e7649c94dd3903de5a1b1728ba22022-12-22T00:26:30ZengKharazmi Universityیافتههای نوین در علوم زیستی2423-63302476-71152020-07-0172133144The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell cultureMonireh Marsafari0Habibollah Samizadeh Lahiji1Babak Rabiei2Ali Ashraf Mehrabi3Yongkun Lv4Peng Xu5 Faculty of Agricultural Science, University of Guilan, Rasht, Iran Faculty of Agricultural Science, University of Guilan, Rasht, Iran Faculty of Agricultural Science, University of Guilan, Rasht, Iran Faculty of Agriculture, University of Ilam, Ilam, Iran Faculty of Biotechnology, Jiangnan University, Jiangsu, China Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, USA Yarrowia lipolytica, as a good cell factory to speed up the production of plant pharmaceutical components, has been considered to be one of the most important and attractive micro-organisms in recent years, due to its high secretion capacity, limited glycosylation, large range of genetic markers and molecular tools. Naringenin, as a central core of flavonoids production, plays important roles both in plants and in the treatment of different types of human diseases. For this purpose, specific naringenin biosynthesis genes from different origins were selected and introduced after comparative expression profiling in Y. lipolytica. This research indicated that chs plays the main role in the production of naringenin, so the increase copy number of this gene in each construct was investigated. The HPLC results confirmed that the construct with 5 copy numbers of chs resulted in 7.14 fold increase of naringenin extracellular titer to 90.16 mg/L in shake flask cultures. The results reported in this study demonstrated that sufficient knowledge of genes involved in the specific biosynthesis pathway, synthetic gene pathway and using Y. lipolytica as a capable and cheap host could help bioengineers to produce significant amounts of pharmaceutical components.http://nbr.khu.ac.ir/article-1-3298-en.htmlgene copy numberhplcmetabolic engineeringmetabolic fluxyeast host |
| spellingShingle | Monireh Marsafari Habibollah Samizadeh Lahiji Babak Rabiei Ali Ashraf Mehrabi Yongkun Lv Peng Xu The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture یافتههای نوین در علوم زیستی gene copy number hplc metabolic engineering metabolic flux yeast host |
| title | The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture |
| title_full | The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture |
| title_fullStr | The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture |
| title_full_unstemmed | The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture |
| title_short | The optimization of Naringenin biosynthesis pathway using Yarrowia lipolitica cell culture |
| title_sort | optimization of naringenin biosynthesis pathway using yarrowia lipolitica cell culture |
| topic | gene copy number hplc metabolic engineering metabolic flux yeast host |
| url | http://nbr.khu.ac.ir/article-1-3298-en.html |
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