Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity
Introduction: Obesity has emerged as one of the main public health problems. This condition triggers a series of hormonal and metabolic changes related to a low-grade chronic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule,...
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| Format: | Article |
| Language: | English |
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Karger Publishers
2021-07-01
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| Series: | Obesity Facts |
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| Online Access: | https://www.karger.com/Article/FullText/516548 |
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| author | Vanessa C.O. Lima Anna B.S. Luz Maria do Socorro M. Amarante Maíra C.J.S. Lima Fabiana M.C. Carvalho Julia B.S. Figueredo Pedro P.A. Santos Christina S. Camillo Fernando V.L. Ladd Bruna L.L. Maciel Adriana F. Uchôa Ana H.A. Morais |
| author_facet | Vanessa C.O. Lima Anna B.S. Luz Maria do Socorro M. Amarante Maíra C.J.S. Lima Fabiana M.C. Carvalho Julia B.S. Figueredo Pedro P.A. Santos Christina S. Camillo Fernando V.L. Ladd Bruna L.L. Maciel Adriana F. Uchôa Ana H.A. Morais |
| author_sort | Vanessa C.O. Lima |
| collection | DOAJ |
| description | Introduction: Obesity has emerged as one of the main public health problems. This condition triggers a series of hormonal and metabolic changes related to a low-grade chronic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs involved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. Methods: Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was administered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromatographic profile and the inhibition assay corroded the purification of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. Results: TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (p = 0.004). The analysis of perirenal adipose tissue showed that the average sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (p = 0.016). In addition, the group that received TTIp had no inflammatory infiltrates. Conclusion: Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflammatory in the evaluated obesity model and can be investigated as a possible adjuvant in obesity therapy. |
| first_indexed | 2024-12-19T16:50:17Z |
| format | Article |
| id | doaj.art-ed8b3863e7314b6fb269438f7b3fa3a1 |
| institution | Directory Open Access Journal |
| issn | 1662-4025 1662-4033 |
| language | English |
| last_indexed | 2024-12-19T16:50:17Z |
| publishDate | 2021-07-01 |
| publisher | Karger Publishers |
| record_format | Article |
| series | Obesity Facts |
| spelling | doaj.art-ed8b3863e7314b6fb269438f7b3fa3a12022-12-21T20:13:33ZengKarger PublishersObesity Facts1662-40251662-40332021-07-0114435736910.1159/000516548516548Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced ObesityVanessa C.O. Lima0Anna B.S. Luz1Maria do Socorro M. Amarante2Maíra C.J.S. Lima3Fabiana M.C. Carvalho4Julia B.S. Figueredo5Pedro P.A. Santos6Christina S. Camillo7Fernando V.L. Ladd8Bruna L.L. Maciel9Adriana F. Uchôa10Ana H.A. Morais11Biochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilBiochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilMorphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilVeterinary course, Potiguar University, Natal, BrazilBiochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilBiochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilMorphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilMorphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilMorphology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilNutrition Postgraduate Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, BrazilBiochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilBiochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, BrazilIntroduction: Obesity has emerged as one of the main public health problems. This condition triggers a series of hormonal and metabolic changes related to a low-grade chronic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs involved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. Methods: Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was administered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromatographic profile and the inhibition assay corroded the purification of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. Results: TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (p = 0.004). The analysis of perirenal adipose tissue showed that the average sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (p = 0.016). In addition, the group that received TTIp had no inflammatory infiltrates. Conclusion: Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflammatory in the evaluated obesity model and can be investigated as a possible adjuvant in obesity therapy.https://www.karger.com/Article/FullText/516548obesitytrypsin inhibitorintestinetoxicityvisceral fatimflammation |
| spellingShingle | Vanessa C.O. Lima Anna B.S. Luz Maria do Socorro M. Amarante Maíra C.J.S. Lima Fabiana M.C. Carvalho Julia B.S. Figueredo Pedro P.A. Santos Christina S. Camillo Fernando V.L. Ladd Bruna L.L. Maciel Adriana F. Uchôa Ana H.A. Morais Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity Obesity Facts obesity trypsin inhibitor intestine toxicity visceral fat imflammation |
| title | Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity |
| title_full | Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity |
| title_fullStr | Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity |
| title_full_unstemmed | Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity |
| title_short | Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity |
| title_sort | tamarind multifunctional protein safety and anti inflammatory potential in intestinal mucosa and adipose tissue in a preclinical model of diet induced obesity |
| topic | obesity trypsin inhibitor intestine toxicity visceral fat imflammation |
| url | https://www.karger.com/Article/FullText/516548 |
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