Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease
Abstract Background The sunflower plants are attacked by serious seed and soil-borne pathogens including charcoal rot disease that caused by Macrophomina phaseolina. This disease has serious damages to sunflower crop. This study aimed to assess the efficacy of Arbuscular mycorrhizal fungus against c...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-023-00747-3 |
| _version_ | 1827633231558606848 |
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| author | Waleed Khalid Ahmed Hutaf A. A. Alsalim Ashwaq Talip Mohammed Hiba Mohammed Youssef |
| author_facet | Waleed Khalid Ahmed Hutaf A. A. Alsalim Ashwaq Talip Mohammed Hiba Mohammed Youssef |
| author_sort | Waleed Khalid Ahmed |
| collection | DOAJ |
| description | Abstract Background The sunflower plants are attacked by serious seed and soil-borne pathogens including charcoal rot disease that caused by Macrophomina phaseolina. This disease has serious damages to sunflower crop. This study aimed to assess the efficacy of Arbuscular mycorrhizal fungus against charcoal rot disease as fungicide alternative. Results Morphological and molecular identification was done, using universal primers for molecular identification. Finally, a greenhouse experiment was conducted, and the length and weight of the plant shoot and root as well as disease incidence and severity percentages were estimated for the treatments infected with the M. phaseolina pathogen. The results showed that molecular identification indicates their subordination to Claroideoglomus etunicatum and Funneliformis mosseae. The greenhouse experiment showed that combined inoculation treatments (C. etunicatum + F. mosseae and C. etunicatum + F. mosseae + M.phaseolina) had the highest averages of length and weight of the plant shoot and root in the non-infected and pathogen-infected (M. phaseolina) treatments. F. mosseae and F. mosseae + M.phaseolina treatments had better effects on plant growth. M. phaseolina pathogen had suppression effects than the C. etunicatum inoculum (C. etunicatum and C. etunicatum + M. phaseolina treatments). The disease incidence and severity percentages decreased significantly in the inoculated plants than in non-inoculated plants. F. mosseae inoculum showed more efficiency in reducing DI and DS than C. etunicatum inoculum, while adding both, resulting in an extra significant reduction. The combined inoculation, which functions as a biofungicide and a biofertilizer, presented a viable biocontrol technique for crop protection. Conclusions Such the AM fungi treatments are considered good and biological safe. The symbiotic relationship of AM fungi with plants can also be utilized to gain successful management of agricultural against soil-borne diseases for getting rid of the negative effects of pesticides. |
| first_indexed | 2024-03-09T14:56:39Z |
| format | Article |
| id | doaj.art-ed8b40a3745a43b69bbc1f6d3b1563a8 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-03-09T14:56:39Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-ed8b40a3745a43b69bbc1f6d3b1563a82023-11-26T14:11:34ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-10-0133111110.1186/s41938-023-00747-3Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot diseaseWaleed Khalid Ahmed0Hutaf A. A. Alsalim1Ashwaq Talip Mohammed2Hiba Mohammed Youssef3Department of Plant Protection, College of Agriculture, Tikrit UniversityDepartment of Biotechnology, College of Science, University of BaghdadDepartment of Plant Protection, College of Agriculture, Tikrit UniversityDepartment of Soil, College of Agriculture, Tikrit UniversityAbstract Background The sunflower plants are attacked by serious seed and soil-borne pathogens including charcoal rot disease that caused by Macrophomina phaseolina. This disease has serious damages to sunflower crop. This study aimed to assess the efficacy of Arbuscular mycorrhizal fungus against charcoal rot disease as fungicide alternative. Results Morphological and molecular identification was done, using universal primers for molecular identification. Finally, a greenhouse experiment was conducted, and the length and weight of the plant shoot and root as well as disease incidence and severity percentages were estimated for the treatments infected with the M. phaseolina pathogen. The results showed that molecular identification indicates their subordination to Claroideoglomus etunicatum and Funneliformis mosseae. The greenhouse experiment showed that combined inoculation treatments (C. etunicatum + F. mosseae and C. etunicatum + F. mosseae + M.phaseolina) had the highest averages of length and weight of the plant shoot and root in the non-infected and pathogen-infected (M. phaseolina) treatments. F. mosseae and F. mosseae + M.phaseolina treatments had better effects on plant growth. M. phaseolina pathogen had suppression effects than the C. etunicatum inoculum (C. etunicatum and C. etunicatum + M. phaseolina treatments). The disease incidence and severity percentages decreased significantly in the inoculated plants than in non-inoculated plants. F. mosseae inoculum showed more efficiency in reducing DI and DS than C. etunicatum inoculum, while adding both, resulting in an extra significant reduction. The combined inoculation, which functions as a biofungicide and a biofertilizer, presented a viable biocontrol technique for crop protection. Conclusions Such the AM fungi treatments are considered good and biological safe. The symbiotic relationship of AM fungi with plants can also be utilized to gain successful management of agricultural against soil-borne diseases for getting rid of the negative effects of pesticides.https://doi.org/10.1186/s41938-023-00747-3Funneliformis mosseaeClaroideoglomus etunicatumMacrophomina phaseolinaArbuscular mycorrhizaBiological controlMolecular diagnosis |
| spellingShingle | Waleed Khalid Ahmed Hutaf A. A. Alsalim Ashwaq Talip Mohammed Hiba Mohammed Youssef Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease Egyptian Journal of Biological Pest Control Funneliformis mosseae Claroideoglomus etunicatum Macrophomina phaseolina Arbuscular mycorrhiza Biological control Molecular diagnosis |
| title | Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| title_full | Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| title_fullStr | Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| title_full_unstemmed | Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| title_short | Evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| title_sort | evaluation of the effectiveness of some mycorrhizal fungi isolates against charcoal rot disease |
| topic | Funneliformis mosseae Claroideoglomus etunicatum Macrophomina phaseolina Arbuscular mycorrhiza Biological control Molecular diagnosis |
| url | https://doi.org/10.1186/s41938-023-00747-3 |
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