MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3
Aims: MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide. Methods: qRT...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2016-08-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/447823 |
| _version_ | 1828801801916252160 |
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| author | Wei Wei Yang Yang Jian Cai Kai Cui Rong xian Li Huan Wang Xiujuan Shang Dong Wei |
| author_facet | Wei Wei Yang Yang Jian Cai Kai Cui Rong xian Li Huan Wang Xiujuan Shang Dong Wei |
| author_sort | Wei Wei |
| collection | DOAJ |
| description | Aims: MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide. Methods: qRT-PCR, Western blot, Transwell assay,luciferase reporter assay were performed in the present study. Results: In this study, miR-30a-5p was found to be significantly downregulated in human colorectal cancer tissue specimens and cell lines compared with non-cancerous tissues and cells. The overexpression of miR-30a-5p inhibited the migratory and invasive abilities of colorectal cancer cells and suppressed the epithelial-mesenchymal transition, a crucial process in metastasis. Bioinformatic algorithms and luciferase reporter assays revealed that integrin β3 (ITGB3) is a direct target of miR-30a-5p. Importantly, overexpression of ITGB3 in colorectal cancer cells rescued these cells from miR-30a-5p-mediated suppression of metastasis and restored the epithelial-mesenchymal transition. Conclusion: Taken together, our study provides the first evidence that miR-30a-5p suppresses colon cancer metastasis through the inhibition of ITGB3. Thus, targeting miR-30a-5p might serve as a promising therapeutic strategy for the treatment of colorectal cancer. |
| first_indexed | 2024-12-12T06:52:47Z |
| format | Article |
| id | doaj.art-ed8b98ae582b48ed8db139bd31e0b9e8 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-12T06:52:47Z |
| publishDate | 2016-08-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-ed8b98ae582b48ed8db139bd31e0b9e82022-12-22T00:34:02ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-08-013931165117610.1159/000447823447823MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3Wei WeiYang YangJian CaiKai CuiRong xian LiHuan WangXiujuan ShangDong WeiAims: MicroRNAs (miRNAs) are dysregulated in a wide range of malignant diseases, confirming their crucial role in tumor metastasis. MiRNA-30a-5p, a member of the miR-30 family, has been implicated in many types of cancers, including colorectal cancer, a leading cause of death worldwide. Methods: qRT-PCR, Western blot, Transwell assay,luciferase reporter assay were performed in the present study. Results: In this study, miR-30a-5p was found to be significantly downregulated in human colorectal cancer tissue specimens and cell lines compared with non-cancerous tissues and cells. The overexpression of miR-30a-5p inhibited the migratory and invasive abilities of colorectal cancer cells and suppressed the epithelial-mesenchymal transition, a crucial process in metastasis. Bioinformatic algorithms and luciferase reporter assays revealed that integrin β3 (ITGB3) is a direct target of miR-30a-5p. Importantly, overexpression of ITGB3 in colorectal cancer cells rescued these cells from miR-30a-5p-mediated suppression of metastasis and restored the epithelial-mesenchymal transition. Conclusion: Taken together, our study provides the first evidence that miR-30a-5p suppresses colon cancer metastasis through the inhibition of ITGB3. Thus, targeting miR-30a-5p might serve as a promising therapeutic strategy for the treatment of colorectal cancer.http://www.karger.com/Article/FullText/447823MiRNA-30a-5pColorectal cancerIntegrin β3Metastasis |
| spellingShingle | Wei Wei Yang Yang Jian Cai Kai Cui Rong xian Li Huan Wang Xiujuan Shang Dong Wei MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 Cellular Physiology and Biochemistry MiRNA-30a-5p Colorectal cancer Integrin β3 Metastasis |
| title | MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 |
| title_full | MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 |
| title_fullStr | MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 |
| title_full_unstemmed | MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 |
| title_short | MiR-30a-5p Suppresses Tumor Metastasis of Human Colorectal Cancer by Targeting ITGB3 |
| title_sort | mir 30a 5p suppresses tumor metastasis of human colorectal cancer by targeting itgb3 |
| topic | MiRNA-30a-5p Colorectal cancer Integrin β3 Metastasis |
| url | http://www.karger.com/Article/FullText/447823 |
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