Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl.
Orchid multiplication is often an impediment to the commercial success of important species. In order to have a sowing technique to evaluate the initial growth of Epidendrum schomburgkii Lindl., seedlings from sexual seed 20 mm high were inoculated in vitro in two basal media: Murashige & Skoog...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Corporación Colombiana de Investigación Agropecuaria (Corpoica)
2022-12-01
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| Series: | Ciencia y Tecnología Agropecuaria |
| Online Access: | https://revistacta.agrosavia.co/index.php/revista/article/view/2526 |
| _version_ | 1827364495715991552 |
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| author | Mario Pinedo-Panduro Edvan Alves-Chagas Francisco Freitas Luz Nadia Masaya Panduro-Tenazoa Pollyana Cardoso Chagas Ricardo Bardales-Lozano Carlos Abanto-Rodríguez Elvis Paredes-Dávila Herman Collazos Saldaña |
| author_facet | Mario Pinedo-Panduro Edvan Alves-Chagas Francisco Freitas Luz Nadia Masaya Panduro-Tenazoa Pollyana Cardoso Chagas Ricardo Bardales-Lozano Carlos Abanto-Rodríguez Elvis Paredes-Dávila Herman Collazos Saldaña |
| author_sort | Mario Pinedo-Panduro |
| collection | DOAJ |
| description |
Orchid multiplication is often an impediment to the commercial success of important species. In order to have a sowing technique to evaluate the initial growth of Epidendrum schomburgkii Lindl., seedlings from sexual seed 20 mm high were inoculated in vitro in two basal media: Murashige & Skoog (MS) and Knudson (KN) at concentrations of 25 %, 50 %, 75 % and 100 %. After 118 days of cultivation, an average of 5.88 live shoots with an average height of 92.80 mm and an average root length of 80.74 mm were obtained. Significant differences were found for the number of live shoots between concentrations, greater stem height and greater root length, as well as in the mean x concentration interaction. A completely randomized statistical design was applied in a 2 x 3 factorial arrangement, and the SPSS, INFOSTAT and BIOSTAT tests were used for the analysis. In general, the values for MS medium were higher compared to KN medium. Regarding the length of the longest stem, we did not find a statistically significant difference between concentrations, but a clear difference in trends (negative for MS and positive for KN). The growth of roots and stems was visibly higher with the MS medium, with an optimal concentration of 25 %. Therefore, when evaluating the formulas and applied doses, it is concluded that the MS medium at a concentration of 25 % offers the best response in the development of propagules.
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| first_indexed | 2024-03-08T08:11:01Z |
| format | Article |
| id | doaj.art-ed8ff9914de44465875285dc98283842 |
| institution | Directory Open Access Journal |
| issn | 2500-5308 |
| language | English |
| last_indexed | 2024-03-08T08:11:01Z |
| publishDate | 2022-12-01 |
| publisher | Corporación Colombiana de Investigación Agropecuaria (Corpoica) |
| record_format | Article |
| series | Ciencia y Tecnología Agropecuaria |
| spelling | doaj.art-ed8ff9914de44465875285dc982838422024-02-02T09:03:11ZengCorporación Colombiana de Investigación Agropecuaria (Corpoica)Ciencia y Tecnología Agropecuaria2500-53082022-12-0123310.21930/rcta.vol23_num3_art:2526Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. Mario Pinedo-Panduro0Edvan Alves-Chagas1Francisco Freitas Luz2Nadia Masaya Panduro-Tenazoa3Pollyana Cardoso Chagas4Ricardo Bardales-Lozano5Carlos Abanto-Rodríguez6Elvis Paredes-Dávila7Herman Collazos Saldaña8Instituto de Investigaciones de la Amazonía Peruana (IIAP)Empresa Brasileira de Pesquisa Agropecuária – Roraima, EMBRAPAUniversidade Federal de Roraima (UFRR)Universidad Nacional Intercultural de la Amazonia (UNIA)Universidade Federal de Roraima (UFRR)Universidad Nacional de la Amazonia Peruana (UNAP)Instituto de Investigaciones de la Amazonía Peruana (IIAP)Instituto de Investigaciones de la Amazonía Peruana (IIAP)Universidad Nacional de Ucayali Orchid multiplication is often an impediment to the commercial success of important species. In order to have a sowing technique to evaluate the initial growth of Epidendrum schomburgkii Lindl., seedlings from sexual seed 20 mm high were inoculated in vitro in two basal media: Murashige & Skoog (MS) and Knudson (KN) at concentrations of 25 %, 50 %, 75 % and 100 %. After 118 days of cultivation, an average of 5.88 live shoots with an average height of 92.80 mm and an average root length of 80.74 mm were obtained. Significant differences were found for the number of live shoots between concentrations, greater stem height and greater root length, as well as in the mean x concentration interaction. A completely randomized statistical design was applied in a 2 x 3 factorial arrangement, and the SPSS, INFOSTAT and BIOSTAT tests were used for the analysis. In general, the values for MS medium were higher compared to KN medium. Regarding the length of the longest stem, we did not find a statistically significant difference between concentrations, but a clear difference in trends (negative for MS and positive for KN). The growth of roots and stems was visibly higher with the MS medium, with an optimal concentration of 25 %. Therefore, when evaluating the formulas and applied doses, it is concluded that the MS medium at a concentration of 25 % offers the best response in the development of propagules. https://revistacta.agrosavia.co/index.php/revista/article/view/2526 |
| spellingShingle | Mario Pinedo-Panduro Edvan Alves-Chagas Francisco Freitas Luz Nadia Masaya Panduro-Tenazoa Pollyana Cardoso Chagas Ricardo Bardales-Lozano Carlos Abanto-Rodríguez Elvis Paredes-Dávila Herman Collazos Saldaña Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. Ciencia y Tecnología Agropecuaria |
| title | Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. |
| title_full | Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. |
| title_fullStr | Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. |
| title_full_unstemmed | Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. |
| title_short | Efecto de las sales de los medios de cultivo Murashige & Skoog y Knudson sobre el establecimiento in vitro de Epidendrum schomburgkii Lindl. |
| title_sort | efecto de las sales de los medios de cultivo murashige skoog y knudson sobre el establecimiento in vitro de epidendrum schomburgkii lindl |
| url | https://revistacta.agrosavia.co/index.php/revista/article/view/2526 |
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