Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos
Ultraviolet (UV) rays can be both harmful and beneficial to humans. This study aimed to investigate the toxicity and safety of ultraviolet C (UVC) exposure in living organisms and the corresponding biodefense molecular mechanisms. Zebrafish embryos, at an early developmental stage (5–6 h post-fertil...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322000513 |
| _version_ | 1798018360306302976 |
|---|---|
| author | Liqing Zang Yasuhito Shimada Hideto Miyake Norihiro Nishimura |
| author_facet | Liqing Zang Yasuhito Shimada Hideto Miyake Norihiro Nishimura |
| author_sort | Liqing Zang |
| collection | DOAJ |
| description | Ultraviolet (UV) rays can be both harmful and beneficial to humans. This study aimed to investigate the toxicity and safety of ultraviolet C (UVC) exposure in living organisms and the corresponding biodefense molecular mechanisms. Zebrafish embryos, at an early developmental stage (5–6 h post-fertilization), were irradiated with increasing UVC dosages using high-efficiency deep-ultraviolet light-emitting diodes (278 nm). Morphological phenotypes including survival rate, hatching rate, heart rate, and malformation rate were evaluated. Compared to un-irradiated controls, all zebrafish embryos exposed to 4.5 mJ/cm2 UVC survived and showed no significant difference in hatching and heart rate. However, 7.5 mJ/cm2 of UVC irradiation caused a significantly decreased survival rate (37.5%) and an increased malformation rate (81.8%). Therefore, 4.5 mJ/cm2 was chosen as the limit dosage that the internal biodefense system of zebrafish embryos can protect against UVC radiation. Transcriptome analysis (RNA sequencing) performed on 3 min and 3 days post-irradiation embryos (4.5 mJ/cm2) revealed the molecular mechanisms underlying the response of zebrafish embryos to irradiation. The embryos quickly responded to UVC-induced stress by activating the p53 signaling pathway. In addition, after 3 days of recuperation, the embryos showed activation of signal transducer and activator of transcription (STAT) signaling pathway. To our knowledge, this is the first study to evaluate the toxicological effects and the molecular mechanism of biodefense in zebrafish embryos upon 278 nm UVC irradiation. |
| first_indexed | 2024-04-11T16:22:44Z |
| format | Article |
| id | doaj.art-ed91d57ec70f4b8f978ed46dc785706b |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T16:22:44Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-ed91d57ec70f4b8f978ed46dc785706b2022-12-22T04:14:19ZengElsevierEcotoxicology and Environmental Safety0147-65132022-02-01231113211Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryosLiqing Zang0Yasuhito Shimada1Hideto Miyake2Norihiro Nishimura3Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan; Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan; Correspondence to: Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan.Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan; Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan; Department of Bioinformatics, Mie University Advanced Science Research Promotion Center, Tsu, Mie 514-8507, JapanGraduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan; Graduate School of Engineering, Mie University, Tsu, Mie 514-8507, JapanGraduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan; Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, JapanUltraviolet (UV) rays can be both harmful and beneficial to humans. This study aimed to investigate the toxicity and safety of ultraviolet C (UVC) exposure in living organisms and the corresponding biodefense molecular mechanisms. Zebrafish embryos, at an early developmental stage (5–6 h post-fertilization), were irradiated with increasing UVC dosages using high-efficiency deep-ultraviolet light-emitting diodes (278 nm). Morphological phenotypes including survival rate, hatching rate, heart rate, and malformation rate were evaluated. Compared to un-irradiated controls, all zebrafish embryos exposed to 4.5 mJ/cm2 UVC survived and showed no significant difference in hatching and heart rate. However, 7.5 mJ/cm2 of UVC irradiation caused a significantly decreased survival rate (37.5%) and an increased malformation rate (81.8%). Therefore, 4.5 mJ/cm2 was chosen as the limit dosage that the internal biodefense system of zebrafish embryos can protect against UVC radiation. Transcriptome analysis (RNA sequencing) performed on 3 min and 3 days post-irradiation embryos (4.5 mJ/cm2) revealed the molecular mechanisms underlying the response of zebrafish embryos to irradiation. The embryos quickly responded to UVC-induced stress by activating the p53 signaling pathway. In addition, after 3 days of recuperation, the embryos showed activation of signal transducer and activator of transcription (STAT) signaling pathway. To our knowledge, this is the first study to evaluate the toxicological effects and the molecular mechanism of biodefense in zebrafish embryos upon 278 nm UVC irradiation.http://www.sciencedirect.com/science/article/pii/S0147651322000513BiodefenseToxicologyRNA-seqUVC-LEDZebrafish embryos |
| spellingShingle | Liqing Zang Yasuhito Shimada Hideto Miyake Norihiro Nishimura Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos Ecotoxicology and Environmental Safety Biodefense Toxicology RNA-seq UVC-LED Zebrafish embryos |
| title | Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos |
| title_full | Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos |
| title_fullStr | Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos |
| title_full_unstemmed | Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos |
| title_short | Transcriptome analysis of molecular response to UVC irradiation in zebrafish embryos |
| title_sort | transcriptome analysis of molecular response to uvc irradiation in zebrafish embryos |
| topic | Biodefense Toxicology RNA-seq UVC-LED Zebrafish embryos |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322000513 |
| work_keys_str_mv | AT liqingzang transcriptomeanalysisofmolecularresponsetouvcirradiationinzebrafishembryos AT yasuhitoshimada transcriptomeanalysisofmolecularresponsetouvcirradiationinzebrafishembryos AT hidetomiyake transcriptomeanalysisofmolecularresponsetouvcirradiationinzebrafishembryos AT norihironishimura transcriptomeanalysisofmolecularresponsetouvcirradiationinzebrafishembryos |