Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon

Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any sp...

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Main Authors: Abdul Rasheed Baloch, Michaela Fučíková, Marek Rodina, Brian Metscher, Tomáš Tichopád, Mujahid Ali Shah, Roman Franěk, Martin Pšenička
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/9/8/333
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author Abdul Rasheed Baloch
Michaela Fučíková
Marek Rodina
Brian Metscher
Tomáš Tichopád
Mujahid Ali Shah
Roman Franěk
Martin Pšenička
author_facet Abdul Rasheed Baloch
Michaela Fučíková
Marek Rodina
Brian Metscher
Tomáš Tichopád
Mujahid Ali Shah
Roman Franěk
Martin Pšenička
author_sort Abdul Rasheed Baloch
collection DOAJ
description Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only.
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spelling doaj.art-ed933723685f4819aa27be33ae2a56092022-12-22T00:31:57ZengMDPI AGBiomolecules2218-273X2019-08-019833310.3390/biom9080333biom9080333Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in SturgeonAbdul Rasheed Baloch0Michaela Fučíková1Marek Rodina2Brian Metscher3Tomáš Tichopád4Mujahid Ali Shah5Roman Franěk6Martin Pšenička7University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of Vienna, Department of Theoretical Biology, Althanstraße 14, 1090 Vienna, AustriaUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicNanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only.https://www.mdpi.com/2218-273X/9/8/333acipensercaviarhyperthermiairon oxide nanoparticlesmicro-CTsterilization
spellingShingle Abdul Rasheed Baloch
Michaela Fučíková
Marek Rodina
Brian Metscher
Tomáš Tichopád
Mujahid Ali Shah
Roman Franěk
Martin Pšenička
Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
Biomolecules
acipenser
caviar
hyperthermia
iron oxide nanoparticles
micro-CT
sterilization
title Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
title_full Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
title_fullStr Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
title_full_unstemmed Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
title_short Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
title_sort delivery of iron oxide nanoparticles into primordial germ cells in sturgeon
topic acipenser
caviar
hyperthermia
iron oxide nanoparticles
micro-CT
sterilization
url https://www.mdpi.com/2218-273X/9/8/333
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