Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon
Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any sp...
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MDPI AG
2019-08-01
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Series: | Biomolecules |
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Online Access: | https://www.mdpi.com/2218-273X/9/8/333 |
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author | Abdul Rasheed Baloch Michaela Fučíková Marek Rodina Brian Metscher Tomáš Tichopád Mujahid Ali Shah Roman Franěk Martin Pšenička |
author_facet | Abdul Rasheed Baloch Michaela Fučíková Marek Rodina Brian Metscher Tomáš Tichopád Mujahid Ali Shah Roman Franěk Martin Pšenička |
author_sort | Abdul Rasheed Baloch |
collection | DOAJ |
description | Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only. |
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language | English |
last_indexed | 2024-12-12T08:06:56Z |
publishDate | 2019-08-01 |
publisher | MDPI AG |
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spelling | doaj.art-ed933723685f4819aa27be33ae2a56092022-12-22T00:31:57ZengMDPI AGBiomolecules2218-273X2019-08-019833310.3390/biom9080333biom9080333Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in SturgeonAbdul Rasheed Baloch0Michaela Fučíková1Marek Rodina2Brian Metscher3Tomáš Tichopád4Mujahid Ali Shah5Roman Franěk6Martin Pšenička7University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of Vienna, Department of Theoretical Biology, Althanstraße 14, 1090 Vienna, AustriaUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicUniversity of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech RepublicNanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only.https://www.mdpi.com/2218-273X/9/8/333acipensercaviarhyperthermiairon oxide nanoparticlesmicro-CTsterilization |
spellingShingle | Abdul Rasheed Baloch Michaela Fučíková Marek Rodina Brian Metscher Tomáš Tichopád Mujahid Ali Shah Roman Franěk Martin Pšenička Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon Biomolecules acipenser caviar hyperthermia iron oxide nanoparticles micro-CT sterilization |
title | Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon |
title_full | Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon |
title_fullStr | Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon |
title_full_unstemmed | Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon |
title_short | Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon |
title_sort | delivery of iron oxide nanoparticles into primordial germ cells in sturgeon |
topic | acipenser caviar hyperthermia iron oxide nanoparticles micro-CT sterilization |
url | https://www.mdpi.com/2218-273X/9/8/333 |
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