Sulpiride Serves, a Substrate for the Gut Microbiome
In the contemporary research world, the intestinal microbiome is now envisioned as a new body organ. Recently, the gut microbiome represents a new drug target in the gut, since various orthologues of intestinal drug transporters are also found present in the microbiome that lines the small intestine...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2021-02-01
|
| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/1559325820987943 |
| _version_ | 1818416632729436160 |
|---|---|
| author | Imran Mukhtar Haseeb Anwar Osman Asghar Mirza Qasim Ali Muhammad Umar Ijaz Michael Hume Bala Krishna Prabhala Arslan Iftikhar Ghulam Hussain Muhammad Umar Sohail Kashif ur Rehman Khan |
| author_facet | Imran Mukhtar Haseeb Anwar Osman Asghar Mirza Qasim Ali Muhammad Umar Ijaz Michael Hume Bala Krishna Prabhala Arslan Iftikhar Ghulam Hussain Muhammad Umar Sohail Kashif ur Rehman Khan |
| author_sort | Imran Mukhtar |
| collection | DOAJ |
| description | In the contemporary research world, the intestinal microbiome is now envisioned as a new body organ. Recently, the gut microbiome represents a new drug target in the gut, since various orthologues of intestinal drug transporters are also found present in the microbiome that lines the small intestine of the host. Owing to this, absorbance of sulpiride by the gut microbiome in an in vivo albino rats model was assessed after the oral administration with a single dose of 20mg/kg b.w. The rats were subsequently sacrificed at 2, 3, 4, 5 and 6 hours post oral administration to collect the gut microbial mass pellet. The drug absorbance by the gut microbiome was determined by pursuing the microbial lysate through RP-HPLC-UV. Total absorbance of sulpiride by the whole gut microbiome and drug absorbance per milligram of microbial pellet were found significantly higher at 4 hours post-administration as compared to all other groups. These results affirm the hypothesis that the structural homology between membrane transporters of the gut microbiome and intestinal epithelium of the host might play an important role in drug absorbance by gut microbes in an in vivo condition. |
| first_indexed | 2024-12-14T11:53:59Z |
| format | Article |
| id | doaj.art-ed949a21a85041c59b29f7679b5ff43c |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-12-14T11:53:59Z |
| publishDate | 2021-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-ed949a21a85041c59b29f7679b5ff43c2022-12-21T23:02:12ZengSAGE PublishingDose-Response1559-32582021-02-011910.1177/1559325820987943Sulpiride Serves, a Substrate for the Gut MicrobiomeImran Mukhtar0Haseeb Anwar1Osman Asghar Mirza2Qasim Ali3Muhammad Umar Ijaz4Michael Hume5Bala Krishna Prabhala6Arslan Iftikhar7Ghulam Hussain8Muhammad Umar Sohail9Kashif ur Rehman Khan10 Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan Department of Drug Design and Pharmacology, University of Copenhagen, Denmark Department of Botany, Government College University, Faisalabad, Pakistan Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan United States Department of Agriculture, College Station, TX, USA Institute of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan Biomedical Research Centre, University of Qatar, Doha, Qatar Department of Pharmaceutical Chemistry, Islamia University Bahawalpur, PakistanIn the contemporary research world, the intestinal microbiome is now envisioned as a new body organ. Recently, the gut microbiome represents a new drug target in the gut, since various orthologues of intestinal drug transporters are also found present in the microbiome that lines the small intestine of the host. Owing to this, absorbance of sulpiride by the gut microbiome in an in vivo albino rats model was assessed after the oral administration with a single dose of 20mg/kg b.w. The rats were subsequently sacrificed at 2, 3, 4, 5 and 6 hours post oral administration to collect the gut microbial mass pellet. The drug absorbance by the gut microbiome was determined by pursuing the microbial lysate through RP-HPLC-UV. Total absorbance of sulpiride by the whole gut microbiome and drug absorbance per milligram of microbial pellet were found significantly higher at 4 hours post-administration as compared to all other groups. These results affirm the hypothesis that the structural homology between membrane transporters of the gut microbiome and intestinal epithelium of the host might play an important role in drug absorbance by gut microbes in an in vivo condition.https://doi.org/10.1177/1559325820987943 |
| spellingShingle | Imran Mukhtar Haseeb Anwar Osman Asghar Mirza Qasim Ali Muhammad Umar Ijaz Michael Hume Bala Krishna Prabhala Arslan Iftikhar Ghulam Hussain Muhammad Umar Sohail Kashif ur Rehman Khan Sulpiride Serves, a Substrate for the Gut Microbiome Dose-Response |
| title | Sulpiride Serves, a Substrate for the Gut Microbiome |
| title_full | Sulpiride Serves, a Substrate for the Gut Microbiome |
| title_fullStr | Sulpiride Serves, a Substrate for the Gut Microbiome |
| title_full_unstemmed | Sulpiride Serves, a Substrate for the Gut Microbiome |
| title_short | Sulpiride Serves, a Substrate for the Gut Microbiome |
| title_sort | sulpiride serves a substrate for the gut microbiome |
| url | https://doi.org/10.1177/1559325820987943 |
| work_keys_str_mv | AT imranmukhtar sulpirideservesasubstrateforthegutmicrobiome AT haseebanwar sulpirideservesasubstrateforthegutmicrobiome AT osmanasgharmirza sulpirideservesasubstrateforthegutmicrobiome AT qasimali sulpirideservesasubstrateforthegutmicrobiome AT muhammadumarijaz sulpirideservesasubstrateforthegutmicrobiome AT michaelhume sulpirideservesasubstrateforthegutmicrobiome AT balakrishnaprabhala sulpirideservesasubstrateforthegutmicrobiome AT arslaniftikhar sulpirideservesasubstrateforthegutmicrobiome AT ghulamhussain sulpirideservesasubstrateforthegutmicrobiome AT muhammadumarsohail sulpirideservesasubstrateforthegutmicrobiome AT kashifurrehmankhan sulpirideservesasubstrateforthegutmicrobiome |