Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences
<p>Abstract</p> <p>Background</p> <p>In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2002-10-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/2/24 |
| _version_ | 1811278332580331520 |
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| author | Kobseva Vera Rakhmanaliev Elian Moisjak Elena Vinokourova Svetlana Klimov Eugene Laimins Laimonis Kisseljov Fjodor Sulimova Galina |
| author_facet | Kobseva Vera Rakhmanaliev Elian Moisjak Elena Vinokourova Svetlana Klimov Eugene Laimins Laimonis Kisseljov Fjodor Sulimova Galina |
| author_sort | Kobseva Vera |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences.</p> <p>Methods</p> <p>Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLASTN program and public databases. To localise the INTs PCR-based screening of GeneBridge4-RH-panel was used.</p> <p>Results</p> <p>Twelve cellular sequences adjacent to integrated HPV16 (INT markers) expressed in squamous cell cervical carcinomas were isolated. For 11 INT markers homologous human genomic sequences were readily identified and 9 of these showed significant homologies to known genes/ESTs. Using the known locations of homologous cDNAs and the RH-mapping techniques, mapping studies showed that the INTs are distributed among different human chromosomes for each tumour sample and are located in regions with the high levels of expression.</p> <p>Conclusions</p> <p>Integration of HPV genomes occurs into the different human chromosomes but into regions that contain highly transcribed genes. One interpretation of these studies is that integration of HPV occurs into decondensed regions, which are more accessible for integration of foreign DNA.</p> |
| first_indexed | 2024-04-13T00:32:51Z |
| format | Article |
| id | doaj.art-ed94ded4750e48c3bf7859dbfa7e8f92 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T00:32:51Z |
| publishDate | 2002-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-ed94ded4750e48c3bf7859dbfa7e8f922022-12-22T03:10:25ZengBMCBMC Cancer1471-24072002-10-01212410.1186/1471-2407-2-24Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequencesKobseva VeraRakhmanaliev ElianMoisjak ElenaVinokourova SvetlanaKlimov EugeneLaimins LaimonisKisseljov FjodorSulimova Galina<p>Abstract</p> <p>Background</p> <p>In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences.</p> <p>Methods</p> <p>Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLASTN program and public databases. To localise the INTs PCR-based screening of GeneBridge4-RH-panel was used.</p> <p>Results</p> <p>Twelve cellular sequences adjacent to integrated HPV16 (INT markers) expressed in squamous cell cervical carcinomas were isolated. For 11 INT markers homologous human genomic sequences were readily identified and 9 of these showed significant homologies to known genes/ESTs. Using the known locations of homologous cDNAs and the RH-mapping techniques, mapping studies showed that the INTs are distributed among different human chromosomes for each tumour sample and are located in regions with the high levels of expression.</p> <p>Conclusions</p> <p>Integration of HPV genomes occurs into the different human chromosomes but into regions that contain highly transcribed genes. One interpretation of these studies is that integration of HPV occurs into decondensed regions, which are more accessible for integration of foreign DNA.</p>http://www.biomedcentral.com/1471-2407/2/24 |
| spellingShingle | Kobseva Vera Rakhmanaliev Elian Moisjak Elena Vinokourova Svetlana Klimov Eugene Laimins Laimonis Kisseljov Fjodor Sulimova Galina Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences BMC Cancer |
| title | Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences |
| title_full | Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences |
| title_fullStr | Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences |
| title_full_unstemmed | Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences |
| title_short | Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences |
| title_sort | human papilloma viruses and cervical tumours mapping of integration sites and analysis of adjacent cellular sequences |
| url | http://www.biomedcentral.com/1471-2407/2/24 |
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