Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats
Background RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. Materials...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2015-10-01
|
| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | http://www.ijfs.ir/article_45327_ad7113c5b49ecf846ee3037ae50168db.pdf |
| _version_ | 1818555161365184512 |
|---|---|
| author | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Amin Tamadon Ali Moghadam Ali Niazi |
| author_facet | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Amin Tamadon Ali Moghadam Ali Niazi |
| author_sort | Atefeh Noroozi |
| collection | DOAJ |
| description | Background RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. Materials and Methods A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawley rats were used in this experimental study. Lactating rats were allotted to 8 equal groups. In 3 groups, the litter size was adjusted to 5, 10, or 15 pups upon parturition. Dams were allowed to suckle their pups continuously until 8 days postpartum. In the other 3 groups, the litter size was adjusted to 5 pups following birth. These pups were separated from the dams for 6 hours on day 8 postpartum, after which the pups were allowed to suckle for 2.5, 5, or 7.5 minutes prior to killing the dams. In 2 groups, lactating rats with 10 and 15 pups were separated from their pups for 6 hours on day 8 postpartum. In these groups, the pups were allowed to suckle their dams for 5 minutes before the dams were killed. All rats were killed on day 8 postpartum and the DMH was removed from each rat. We evaluated RFRP mRNA expression using realtime polymerase chain reaction (PCR). Results The expression of RFRP mRNA in the DMH increased with increased litter size and suckling intensity compared to the controls. The effect of suckling intensity on the expression of RFRP mRNA was more pronounced compared to the litter size. Conclusion Increased litter size and suckling intensity stimulated RFRP mRNA expression in the DMH which might contribute to lactation anestrus in rats. |
| first_indexed | 2024-12-12T09:49:35Z |
| format | Article |
| id | doaj.art-ed95fae4fff44f0091069f3198e6b113 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-12T09:49:35Z |
| publishDate | 2015-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-ed95fae4fff44f0091069f3198e6b1132022-12-22T00:28:18ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782015-10-019338038610.22074/ijfs.2015.455445327Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in RatsAtefeh Noroozi0Mohammad Reza Jafarzadeh Shirazi1Amin Tamadon2Ali Moghadam3Ali Niazi4Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranDepartment of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, IranTransgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBiotechnology Institute, College of Agriculture, Shiraz University, Shiraz, IranBiotechnology Institute, College of Agriculture, Shiraz University, Shiraz, IranBackground RFamide-related peptide-3 (RFRP-3) inhibits gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in rats. This study evaluates the effects of litter size and suckling intensity on RFRP mRNA expression in the dorsomedial hypothalamic nucleus (DMH) of rats. Materials and Methods A total of 32 pregnant and 4 non-lactating ovariectomized (control group) Sprague-Dawley rats were used in this experimental study. Lactating rats were allotted to 8 equal groups. In 3 groups, the litter size was adjusted to 5, 10, or 15 pups upon parturition. Dams were allowed to suckle their pups continuously until 8 days postpartum. In the other 3 groups, the litter size was adjusted to 5 pups following birth. These pups were separated from the dams for 6 hours on day 8 postpartum, after which the pups were allowed to suckle for 2.5, 5, or 7.5 minutes prior to killing the dams. In 2 groups, lactating rats with 10 and 15 pups were separated from their pups for 6 hours on day 8 postpartum. In these groups, the pups were allowed to suckle their dams for 5 minutes before the dams were killed. All rats were killed on day 8 postpartum and the DMH was removed from each rat. We evaluated RFRP mRNA expression using realtime polymerase chain reaction (PCR). Results The expression of RFRP mRNA in the DMH increased with increased litter size and suckling intensity compared to the controls. The effect of suckling intensity on the expression of RFRP mRNA was more pronounced compared to the litter size. Conclusion Increased litter size and suckling intensity stimulated RFRP mRNA expression in the DMH which might contribute to lactation anestrus in rats.http://www.ijfs.ir/article_45327_ad7113c5b49ecf846ee3037ae50168db.pdfmrnasuckling intensitydorsomedial hypothalamic nucleuslactationrat |
| spellingShingle | Atefeh Noroozi Mohammad Reza Jafarzadeh Shirazi Amin Tamadon Ali Moghadam Ali Niazi Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats International Journal of Fertility and Sterility mrna suckling intensity dorsomedial hypothalamic nucleus lac tation rat |
| title | Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats |
| title_full | Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats |
| title_fullStr | Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats |
| title_full_unstemmed | Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats |
| title_short | Increased Litter Size and Suckling Intensity Stimulate mRNA of RFamide-related Peptide in Rats |
| title_sort | increased litter size and suckling intensity stimulate mrna of rfamide related peptide in rats |
| topic | mrna suckling intensity dorsomedial hypothalamic nucleus lac tation rat |
| url | http://www.ijfs.ir/article_45327_ad7113c5b49ecf846ee3037ae50168db.pdf |
| work_keys_str_mv | AT atefehnoroozi increasedlittersizeandsucklingintensitystimulatemrnaofrfamiderelatedpeptideinrats AT mohammadrezajafarzadehshirazi increasedlittersizeandsucklingintensitystimulatemrnaofrfamiderelatedpeptideinrats AT amintamadon increasedlittersizeandsucklingintensitystimulatemrnaofrfamiderelatedpeptideinrats AT alimoghadam increasedlittersizeandsucklingintensitystimulatemrnaofrfamiderelatedpeptideinrats AT aliniazi increasedlittersizeandsucklingintensitystimulatemrnaofrfamiderelatedpeptideinrats |