Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays
Lyme borreliosis is a vector-borne disease in humans and animals caused by bacteria from the <i>Borrelia burgdorferi</i> sensu lato complex (<i>Bb</i>sl). The possible transmission of <i>Bb</i>sl from companion animals to humans via ticks makes this disease import...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/11/633 |
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| author | Sophie Charlotte Doff Jasmin Wenderlein Anna Wiesinger Stephanie Hiereth Sebastian Ulrich Reinhard K. Straubinger |
| author_facet | Sophie Charlotte Doff Jasmin Wenderlein Anna Wiesinger Stephanie Hiereth Sebastian Ulrich Reinhard K. Straubinger |
| author_sort | Sophie Charlotte Doff |
| collection | DOAJ |
| description | Lyme borreliosis is a vector-borne disease in humans and animals caused by bacteria from the <i>Borrelia burgdorferi</i> sensu lato complex (<i>Bb</i>sl). The possible transmission of <i>Bb</i>sl from companion animals to humans via ticks makes this disease important in terms of One Health approaches. Thus, early and accurate diagnosis and treatment are of utmost importance. Today’s standard for the detection of specific antibodies against <i>Bb</i>sl is a two-tiered test system based on an ELISA for screening combined with a line immunoassay (LIA) for confirmation. In this study, 200 canine and 200 equine serum samples with known antibody status were tested with two different LIAs (A and B). Results were compared regarding sensitivity, specificity, the diagnostic outcome for dogs and horses, as well as operability of the test. The results for canine serum samples corresponded to 94.0%, making both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the agreement of both tests was 65.5%, displaying the challenge equine samples still provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals on the LIA. The operability of both LIAs was equally user-friendly. Regarding the tests’ evaluation, the scanning process provided by LIA A was a major advantage considering the comparability of the tests. |
| first_indexed | 2024-03-09T17:56:43Z |
| format | Article |
| id | doaj.art-ed964b28df6a46c494d8184cec7620a7 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T17:56:43Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-ed964b28df6a46c494d8184cec7620a72023-11-24T10:17:44ZengMDPI AGVeterinary Sciences2306-73812022-11-0191163310.3390/vetsci9110633Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line ImmunoassaysSophie Charlotte Doff0Jasmin Wenderlein1Anna Wiesinger2Stephanie Hiereth3Sebastian Ulrich4Reinhard K. Straubinger5Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyChair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyChair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyChair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyChair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyChair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, GermanyLyme borreliosis is a vector-borne disease in humans and animals caused by bacteria from the <i>Borrelia burgdorferi</i> sensu lato complex (<i>Bb</i>sl). The possible transmission of <i>Bb</i>sl from companion animals to humans via ticks makes this disease important in terms of One Health approaches. Thus, early and accurate diagnosis and treatment are of utmost importance. Today’s standard for the detection of specific antibodies against <i>Bb</i>sl is a two-tiered test system based on an ELISA for screening combined with a line immunoassay (LIA) for confirmation. In this study, 200 canine and 200 equine serum samples with known antibody status were tested with two different LIAs (A and B). Results were compared regarding sensitivity, specificity, the diagnostic outcome for dogs and horses, as well as operability of the test. The results for canine serum samples corresponded to 94.0%, making both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the agreement of both tests was 65.5%, displaying the challenge equine samples still provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals on the LIA. The operability of both LIAs was equally user-friendly. Regarding the tests’ evaluation, the scanning process provided by LIA A was a major advantage considering the comparability of the tests.https://www.mdpi.com/2306-7381/9/11/633antibody<i>Borrelia burgdorferi</i> sensu latocanineequineserum diagnosisline immunoassay |
| spellingShingle | Sophie Charlotte Doff Jasmin Wenderlein Anna Wiesinger Stephanie Hiereth Sebastian Ulrich Reinhard K. Straubinger Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays Veterinary Sciences antibody <i>Borrelia burgdorferi</i> sensu lato canine equine serum diagnosis line immunoassay |
| title | Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays |
| title_full | Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays |
| title_fullStr | Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays |
| title_full_unstemmed | Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays |
| title_short | Detection of <em>Borrelia burgdorferi</em> sensu-lato-Specific Antibodies in Sera of Canine and Equine Origin—A Comparative Study with Two Line Immunoassays |
| title_sort | detection of em borrelia burgdorferi em sensu lato specific antibodies in sera of canine and equine origin a comparative study with two line immunoassays |
| topic | antibody <i>Borrelia burgdorferi</i> sensu lato canine equine serum diagnosis line immunoassay |
| url | https://www.mdpi.com/2306-7381/9/11/633 |
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