Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299
Non-small cell lung cancer (NSCLC) is an aggressive lung cancer accounting for approximately 85% of all lung cancer patients. For the patients with Stages IIIA, IIIB, and IIIC, the 5-year survival is low though with the combination with radiotherapy and chemotherapy. In addition, the occurrence of t...
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MDPI AG
2021-09-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/43/3/85 |
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| author | Kaori Tsutsumi Ayaka Chiba Yuta Tadaki Shima Minaki Takahito Ooshima Haruka Takahashi |
| author_facet | Kaori Tsutsumi Ayaka Chiba Yuta Tadaki Shima Minaki Takahito Ooshima Haruka Takahashi |
| author_sort | Kaori Tsutsumi |
| collection | DOAJ |
| description | Non-small cell lung cancer (NSCLC) is an aggressive lung cancer accounting for approximately 85% of all lung cancer patients. For the patients with Stages IIIA, IIIB, and IIIC, the 5-year survival is low though with the combination with radiotherapy and chemotherapy. In addition, the occurrence of tumor cells (repopulated tumors) that survive irradiation remains a challenge. In our previous report, we subcloned the radiation-surviving tumor cells (IR cells) using the human NSCLC cell line, H1299, and found that the expression of neuropilin-1 (<i>NRP</i>-1) was upregulated in IR cells by the microarray analysis. Here, we investigated the contribution of neuropilin-1 to changes in the characteristics of IR cells. Although there were no differences in angiogenic activity in the tube formation assay between parental and IR cells, the cell motility was increased in IR cells compared to parental cells in the cell migration assay. This enhanced cell motility was suppressed by pretreatment with anti-NRP-1 antibody. Although further studies are necessary to identify other molecules associated with NRP-1, the increase in cellular motility in IR cells might be due to the contribution of NRP-1. Inhibition of NRP-1 would help control tumor malignancy in radiation-surviving NSCLC. |
| first_indexed | 2024-03-10T04:24:21Z |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T04:24:21Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-ed9717b893e148fe8bedbaba9420f9a42023-11-23T07:43:25ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452021-09-014331203121110.3390/cimb43030085Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299Kaori Tsutsumi0Ayaka Chiba1Yuta Tadaki2Shima Minaki3Takahito Ooshima4Haruka Takahashi5Faculty of Health Sciences, Hokkaido University, Sapporo 060-0812, JapanDivision of Radiology and Nuclear Medicine, Sapporo Medical University Hospital, Sapporo 060-8556, JapanDepartment of Radiological Technology, Saiseikai Otaru Hospital, Otaru 047-0008, JapanDepartment of Radiological Technology, Sapporo Spine Clinic, Sapporo 060-0042, JapanDepartment of Radiological Technology, Tomakomai City Hospital, Tomakomai 053-8567, JapanDepartment of X-ray Technology, Sapporo City General Hospital, Sapporo 060-8604, JapanNon-small cell lung cancer (NSCLC) is an aggressive lung cancer accounting for approximately 85% of all lung cancer patients. For the patients with Stages IIIA, IIIB, and IIIC, the 5-year survival is low though with the combination with radiotherapy and chemotherapy. In addition, the occurrence of tumor cells (repopulated tumors) that survive irradiation remains a challenge. In our previous report, we subcloned the radiation-surviving tumor cells (IR cells) using the human NSCLC cell line, H1299, and found that the expression of neuropilin-1 (<i>NRP</i>-1) was upregulated in IR cells by the microarray analysis. Here, we investigated the contribution of neuropilin-1 to changes in the characteristics of IR cells. Although there were no differences in angiogenic activity in the tube formation assay between parental and IR cells, the cell motility was increased in IR cells compared to parental cells in the cell migration assay. This enhanced cell motility was suppressed by pretreatment with anti-NRP-1 antibody. Although further studies are necessary to identify other molecules associated with NRP-1, the increase in cellular motility in IR cells might be due to the contribution of NRP-1. Inhibition of NRP-1 would help control tumor malignancy in radiation-surviving NSCLC.https://www.mdpi.com/1467-3045/43/3/85non-small cell lung cancerradiotherapyrepopulated tumorneuropilinmotility |
| spellingShingle | Kaori Tsutsumi Ayaka Chiba Yuta Tadaki Shima Minaki Takahito Ooshima Haruka Takahashi Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 Current Issues in Molecular Biology non-small cell lung cancer radiotherapy repopulated tumor neuropilin motility |
| title | Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 |
| title_full | Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 |
| title_fullStr | Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 |
| title_full_unstemmed | Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 |
| title_short | Contribution of Neuropilin-1 in Radiation-Survived Subclones of NSCLC Cell Line H1299 |
| title_sort | contribution of neuropilin 1 in radiation survived subclones of nsclc cell line h1299 |
| topic | non-small cell lung cancer radiotherapy repopulated tumor neuropilin motility |
| url | https://www.mdpi.com/1467-3045/43/3/85 |
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